Protein family

Qkine is committed to manufacturing enhanced-quality bioactive proteins to improve scientific outcomes and reproducibility. We provide a complete portfolio of animal origin-free and carrier protein-free growth factors and cytokines for stem cell and organoid culture. Our products support applications including iPSC and ESC maintenance and differentiation, 3D cell culture, organoids, neural stem cell culture and emerging sectors such as organ-on-chip and cultivated meat, fish and dairy.

Growth factor and cytokine for stem cell and organoid culture

Colony-stimulating factor

CSF family

Colony-stimulating factors family, also known as hematopoietic growth factors, include granulocyte-macrophage-CSF (GM-CSF), macrophage colony formation CSF (M-CSF), granulocyte-CSF (G-CSF), and multi-CSF, which is also referred to as interleukin-3 (IL-3). These CSFs serve various functions such as promoting cell proliferation, facilitating cell maturation, and encouraging cell differentiation.

Dickkopf protein

DKK family

The Dickkopf (DKK) family comprises four primary secreted proteins: DKK-1, DKK-2, DKK-3, and DKK-4, along with soggy (Sgy-1 or DKKL1), a protein related to DKK-3. These glycoproteins play an important role in numerous biological processes, especially in the regulation of the Wnt signaling pathway.

Epidermal growth
factor

EGF family

The Epidermal Growth Factor (EGF) family includes EGF and Neuregulins. EGF plays crucial roles in the regulation of cell proliferation, survival, differentiation, and migration. Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR.

Fibroblast growth
factor

FGF superfamily

Fibroblast growth factors (FGFs) are a family of 22 proteins (18 secreted proteins and 4 intracellular FGFs) involved in many developmental processes. Fibroblast Growth Factors stimulate growth or differentiation of cells of mesodermal or neuroectodermal origin. FGF is also used widely in organoid culture. Qkine portfolio includes many members: FGF-2, FGF-4, FGF-7 (KGF), FGF-8b and FGF-10.

Hedgehog
protein

Hedgehog family

The Hedgehog protein family includes 3 key members: sonic hedgehog (Shh), Indian hedgehog (Ihh), and desert hedgehog (Dhh). These proteins play diverse roles as signaling agents, participating in various biological processes such as cell differentiation, proliferation, and survival. Shh protein has many applications in the neural stem cell field, where it plays a significant role in iPSC towards motor neurons and interneurons.

Hepatocyte growth
factor

HGF family

Hepatocyte growth factor (HGF), also known as Scatter factor, acts as a multifunctional cytokine on cells including endothelial and epithelial cells, melanocytes, and keratinocytes. HGF is now recognized as a key player in mediating protection against various inflammatory and autoimmune diseases.

All our recombinant proteins are animal origin-free
and come with Bioactivity. Guaranteed.

Insulin-like growth
factor

IGF family

The insulin-like growth factors (IGFs) are proteins with a similar structure to insulin. This family includes ligands and insulin, receptors and insulin receptor, and IGF-binding proteins. The insulin-like growth factor family plays important roles in differentiation, cellular metabolism, proliferation, apoptosis and transformation.

Interleukin
protein

IL superfamily

IL superfamily is a large group of cytokines, including proteins known as interleukins and interleukin receptors. Members of this superfamily play pivotal roles in diverse biological processes such as immunity, inflammation, and hematopoiesis. Subgroups within the interleukin superfamily include the IL-1, IL-6, IL-10, IL-12, and IL-17 subfamilies, along with additional interleukins and receptors.

Neurotrophin
protein

Neurotrophin family

The Neurotrophin family includes Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin-3, and Neurotrophin-4. Functioning as growth factors, Neurotrophins play crucial roles in regulating the development, survival, and maintenance of neurons within both the central and peripheral nervous systems, thereby contributing significantly to neural plasticity and overall nervous system function.

Platelet-derived growth factor

PDGF family

The PDGF family is composed of PDGF-A, PDGF-B, PDGF-C, and PDGF-D. These four PDGFs remain inactive in their monomeric forms. Upon activation, PDGFs interact with protein tyrosine kinase receptors known as PDGF receptor-α and -β.
PDGFs play essential roles in controlling cell survival, proliferation, and migration both during development and the process of wound healing.

R-spondin
protein

R-spondin family

R-spondin family (RSPO) includes 4 secreted glycoproteins (R-spondin 1, R-spondin 2, R-spondin 3, R-spondin 4). Implicated in the activation and regulation of the Wnt signaling pathway, RSPO proteins contribute significantly to various biological processes throughout embryonic development, adult tissue maintenance, and the onset of disease. This family of secreted proteins is also used widely in organoid culture.

Transforming growth factor beta

TGF beta superfamily

Members of the TGF-beta superfamily, such as Activins, TGF-beta 1, TGF-beta 2, TGF-beta 3, GDF, GDNF, and BMP, play crucial roles in governing various developmental and physiological processes. Transforming growth factor beta superfamily proteins contribute to the regulation of fundamental cellular properties and vital cellular processes, such as cell growth, differentiation, proliferation, survival, apoptosis, morphogenesis, and tissue remodeling.

Purchase in bulk and save

If you need large quantities, contact us for a bulk quote. We can scale up the production of any protein to meet your needs and offer additional discounts on bulk orders.

All our proteins are equally or more bioactive than those from other dominant suppliers

Bioactivity of recombinant BMP-4 protein was determined by a luciferase assay.
Qkine E. coli expressed BMP-4 (Qk038, green) has a higher bioactivity (EC50=19.4 pM) than alternative mammalian expressed (Supplier A, blue, EC50=133.5 pM) and E. coli expressed (Supplier B, black, EC50=43.9 pM) BMP-4.

At Qkine, we test and validate the biological activity of all our growth factors against other suppliers’ proteins to ensure that we provide you with the highest level of biological activity.

Bioactivity comparison between leading suppliers shows Qk038 animal-free BMP-4 (expressed in E. coli) has a higher bioactivity than microbial and mammalian expressed BMP-4 from alternative suppliers.

All our proteins adhere to the Nine-point Qkine Quality Commitment

1. Certified animal-free with full traceability

2. Manufactured within our ISO 9001:2015-certified facility with stringent in-process control measures

3. Rigorously tested for accurate protein recovery from vials

4. Subject to quantitative bioactivity analysis with integrated lot-to-lot reproducibility criteria

5. Proven to have industry-leading protein purity levels

6. Subject to comprehensive identity analysis for structurally complex bioactive proteins

7. Assayed to ensure industry-leading low endotoxin levels

8. Rigorously tested for sterility and mycoplasma contamination for confident use in cell-culture applications

9. From concept to manufacture, our proteins are designed to be the best

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Quality control

Animal origin-free proteins quality control

To ensure that your results are reliable and reproducible every time, we have stringent quality control procedures in place.

Quality policy

Qkine ISO 9001: 2015 certification

All proteins are manufactured in compliance with an enhanced ISO 9001:2015 quality management system

Animal-free

Qkine manufactures recombinant proteins in a dedicated animal-free laboratory.

Contact our expert team

Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.

Raising the standard in bioactive protein manufacturing and innovation

Our science team is here to help, please contact us if you have any questions.