• high purity growth factors for organoid culture
  • replacements for conditioned media
  • protein innovation for improved cultures
  • fully defined and animal-free

Organoids are 3D assemblies of cells, containing multiple cell types, arranged in a similar structure to the modelled tissue, but on a micro-scale. Organoids represent a more physiologically relevant model of tissues than 2D and mono-culture systems. As organoids recapitulate tissue-specific features, they are used in the study of tissue development and maintenance, stem cell characteristics, cellular interactions, precision medicine, disease modelling and drug discovery.

adult stem cell-derived organoids

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induced pluripotent stem cell-derived organoids

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problems with you organiod cultures?

Troubleshoot your organoid and stem cell cultures with Qkine’s Super Scientist! Are your growth factors the reason your experiments fail? Can you use engineered or niche-specific growth factors to improve your organoid culture? Super Scientist flies in with easy fixes and questions to ask yourself – do you need to ditch the conditioned media, play around with growth factor combinations, switch noggin and gremlin, or be bold and explore new engineered proteins.

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improve your organoids with innovative growth factor forms

high affinity LGR5 receptor-binding R-spondin 1 LR5

R-spondin 1 LR5 specifically activates Wnt signalling in lgr5+ stem cell population

Part of the Pioneering Protein range, R-spondin 1 LR5 protein (Qk031) is a specialized form of R-spondin 1, developed in the lab of Marc de la Roche (University of Cambridge), that specifically binds to the LGR5 receptor.

Wild-type R-spondin 1 (Qk006) is a ligand for the leucine-rich repeat-containing G-protein coupled receptors (LGR) 4-6. Binding of R-spondin 1 to its coreceptors, LGR4-6 and ZNRF3, releases the inhibition ZNRF3 imposes on Wnt signalling.

In epithelial tissues, LGR5 marks the stem cell population. This unique engineered form of R-spondin 1, R-spondin1 LR5, activates Wnt signalling only in the LGR5+ stem cell population.

R-spondin1 LR5 supports organoid survival and growth in intestinal organoid culture.   As LGR5 specifically marks stem cells and is not found on transit amplifying cells, lower crypt multiplicity is seen in organoid cultures with R-spondin1 LR5 (in comparison with wild-type R-spondin1).  This supports the hypothesis that this engineered form is acting specifically on stem cells.  Do you have an organoid system where targeting LGR5+ stem cells may enhance the characteristics of your culture?  Contact us if you would like to collaborate to explore these exciting advances.

All organoid data from the laboratory of Marc de la Roche and reproduced with permission (© Marc de la Roche, 2020)

learn more about R-spondin1 LR5

organoid survival is maintained

Organoid survival

crypt multiplicity is reduced

Crypt multiplicity
Mouse intestinal organoid morphology

big improvements from smallest HGF natural isoform NK1

The NK1 isoform is the smallest naturally occurring splice variant of HGF, it comprises the HGF N-terminal and first kringle domain. This isoform promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml, resulting in highly homogeneous expression of the hepatic marker, HNF4α. Qkine HGF NK1 is also completely animal-free (AOF), making it suitable for chemically defined media and reproducible scale-up.

find out more about HGF NK1

we’re here to help so please ask us any questions about growth factors for organoid culture, proteins in our R&D pipeline or indeed anything else …

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