Growth factors for defined organoid culture media

Organoids are 3D assemblies of cells, containing multiple cell types, arranged in a similar structure to the modelled tissue, but on a micro-scale. Organoids represent a more physiologically relevant model of tissues than 2D and mono-culture systems. As organoids recapitulate tissue-specific features, they are used in the study of tissue development and maintenance, stem cell characteristics, cellular interactions, precision medicine, disease modelling and drug discovery.  Due to long periods in culture, it is important that all growth factors for organoid culture give highly consistent biological activity.  Where possible, consider replacing conditioned media with recombinant proteins such as high purity R-spondin 1 and noggin to improve reproducibility and scale-up.

Products for organoid culture

Intestinal organoids

EGF (Qk011)
noggin (Qk034 or Qk033)
R-spondin 1 (Qk006)
FGF-2 / bFGF (Qk027)
IGF-1 (Qk047)

Cardiac organoids

TGF-B1 (Qk010)
FGF-2 145 aa (Qk025)
FGF-2 154 aa (Qk027)
activin A (Qk001)
BMP-4 (Qk038)

Lung organoids

activin A (Qk001)
FGF-4 (Qk004)
FGF-10 (Qk003)
noggin (Qk033 or Qk034)

Breast organoids

R-spondin 3 (Qk032)
NRG-1 (Qk045)
FGF-7 / KGF (Qk046)
FGF-10 (Qk003)
EGF (Qk011)
noggin (Qk034)
HGF NK1 (Qk013)

Liver organoids

activin A (Qk001)
OSM (Qk049)
HGF NK1 (Qk013)
BMP4  (Qk038)
EGF (Qk011)
FGF-2 145 aa (Qk025)
FGF-2 154 aa (Qk027)
FGF-7 / KGF (Qk046)

Brain organoids

BDNF (Qk050)
GDNF (Qk051)
TGF-B1 PLUS (Qk010)
IGF-1 (Qk047)

Trouble-shooting organoid culture, some inspiration!

Can you use engineered or niche-specific growth factors to improve your organoid culture? Super Scientist flies in with easy fixes and questions to ask yourself – do you need to replace the conditioned media, explore new growth factor combinations, switch noggin and gremlin, or be bold and explore new engineered proteins.

Our scientists are here to help.

Please contact us with questions, product suggestions and ideas.
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