Growth Factors

made for stem cells

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Animal-free

We control every step of protein manufacture ensuring all our products are completely animal-free

Total transparency

Stringent purity and bioactivity testing data for all proteins – know what you are giving your cells

Protein innovation

Our R&D team are dedicated to solving stem cell culture challenges with optimised forms and animal-free firsts

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Bioactivity graph showing the EC50 of 0.55 ng/ml (14.4 pM) for Qkine recombinant human VEGF 165 is higher than an alternative supplier sample tested in parallel

New

Recombinant human VEGF 165

Human vascular endothelial growth factor 165 (VEGF 165) is used in culturing primary vascular endothelial cells (like HUVEC and HMVEC) and promoting the proliferation and differentiation of iPSC-derived endothelial cells. Vasculature in various types of organoid models relies on the efficiency of recombinant human VEGF-165 protein. It regulates mesenchymal stem cell differentiation and helps in maintaining hematopoietic stem cells and tumor cells in culture.
Key features of Qkine VEGF 165:
• Higher bioactivity than an alternative supplier
• High purity 38 kDa homodimer
• Suitable for chemically-defined media
• Carrier-protein free
• Animal-free (ADCF)
• Cost-effective

Shh protein (Qk055)

New

Recombinant human Shh

Human Sonic hedgehog (Shh), with its diverse functions, is used to enhance the specification of hPSCs towards a dopaminergic fate and has a significant role in generating hPSC-derived GABAergic medium spiny neurons, motor neurons, serotonergic neurons, midbrain organoids, and ventral forebrain organoids.
Key features of Qkine Shh:
• Highly pure and bioactive 19.8 kDa N-terminal fragment (C24II)
• Suitable for chemically-defined media
• Carrier-protein free
• Animal-free
• Cost-effective

#FridayRead

In this week’s #FridayRead, Mohamed et al. present the method to generate larger numbers of human Midbrain Organoids (hMOs), in a manner that decreases variability whilst maintaining the viability of these hMOs over time – addressing the reproducibility challenge of the stem cell field.

Mohamed et al., Early Drug Discovery Unit (EDDU), Montreal Neurological Institute-Hospital

#TechTip

Always reconstitute TGF beta family proteins using a low pH (We use 10mM HCl) – these proteins will precipitate in a physiological buffer. The low pH will also aid the maintenance of the correct disulfide structure by minimizing disulfide bond exchange reactions. Learn more about handling and storing proteins with Qkine.

Qkine Tech Support Team

Trusted by experts

“Our lab has been using FGF2 and activin A from Qkine in stem cell cultures for many months now. Our epiblast stem cells and trophoblast stem cells never looked better. Qkine has provided us with exceptional customer service and most importantly top-quality, affordable growth factors. We will be definitely using their products in the future.”
Prof Jan Zylicz, Novo Nordisk Foundation, University of Copenhagen

Recombinant human activin A PLUS, Qk005, and recombinant zebrafish Fgf2, Qk002,  feature in the recent publication:
Kinoshita, M. et al. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency. Cell Stem Cell (2020) doi:10.1016/j.stem.2020.11.005.

Austin Smith Lab, Cambridge Stem Cell Institute / University of Exeter
“We have used GDF15 from Qkine in cellular experiments and in vivo and it performs very well. Qkine staff are communicative and efficient and delivery times are excellent.”
Prof Stephen O’Rahilly, University of Cambridge, UK

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