Recombinant human / mouse FGF-8a protein (Qk059)
Fibroblast growth factor 8a (FGF-8a) is a member of the FGF family and plays a key role in the regulation of embryogenesis, cellular proliferation, differentiation, and migration. FGF8a is often used for the differentiation of induced pluripotent stem cells, embryonic stem cells, and neural stem cells.
FGF8a is a spliced form of FGF8, a heparin-binding protein that targets mammary and non-mammary cells expressing the FGF receptors. A 21.3kDa highly pure, bioactive recombinant protein produced in an animal-free expression system. This protein is carrier-free, tag-free, and non-glycosylated to ensure a pure homogenous protein with exceptional lot-to-lot consistency. Qk059 is suitable for enhanced reproducibility and physiologically relevant cultures.
FGF-8a activity is determined using the Promega serum response element luciferase reporter assay (*) in HEK293T cells. EC50 = 110 ng/ml (5.2 nM).
Cells are treated in triplicate with a serial dilution of FGF-8a in the presence of 10 µg/ml heparin for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk059 lot #104406.
FGF-8a (Qk059) migrates as a single band at 21.3 kDa in non-reducing (NR) conditions and upon reduction (R).
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk059 batch #104412.
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
FGF-8a is a heparin-binding protein and an isoform of FGF-8 belonging to a family of fibroblast growth factors (FGF) . It was originally discovered as an essential growth factor for the androgen-dependent growth of mouse mammary carcinoma cells . In mouse, there are eight spliced protein isoforms of FGF8 (a-h) whereas, in humans, there are four alternate spliced protein isoforms namely FGF-8a, FGF-8b, FGF-8e, and FGF-8f. These four FGF8 isoforms (a, b, e and f) are highly conserved between humans and mice. Human and murine FGF-8a and FGF-8b show 100% homology  whereas there is a 98% identity with human and murine FGF8e and FGF8f . Human FGF-8a, a monomeric protein has a molecular weight of 21.3kDa with 182 amino acid (aa) residues covering the signal sequence domain, N-terminal domain, FGF domain and proline-rich C terminal sequence . Its three-dimensional structure is composed of two antiparallel beta-sheet and several alpha-helices. The protein contains a conserved heparin-binding domain that is essential for its biological activity. This domain binds to heparin sulfate proteoglycans on the cell surface and helps to localize FGF8a to its target cells.
FGF-8, including the spliced forms, functions by binding the FGF receptors (FGFR) to activate the Ras/MAPK signaling pathway, a key pathway that contributes to several cellular processes. In general, the FGF family is involved in broad cellular and biological processes including cell proliferation, differentiation, survival, and apoptosis [6-8].
Functionally, FGF-8a has been shown to play a major role during prenatal development. It is widely expressed during embryogenesis and is a key player in epithelial-mesenchymal transitions . During gastrulation, it contributes to the organization and induction role and regulates the patterning of organs in the embryos. These organs include the brain, eye, ear, limb, and heart [10-12].
Although, FGF-8 isoforms work in a coordinated and concerted manner, findings have suggested that they also have distinct key roles. FGF-8a is required for morphogenesis and neurogenesis . A study using transgenic mice showed FGF-8a expands the midbrain while FGF-8b showed a transformational activity by transforming the midbrain into the cerebellum .
Furthermore, FGF-8a has been used to generate embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)-derived ventral midbrain dopaminergic (DA) neurons . FGF-8a has also been shown to play a role in neuronal induction during neural development and neurite outgrowth from spiral ganglion neurons in vitro [15-16].
Whilst there is limited expression of FGF-8 and its isoforms in the normal adult, increasing studies have shown the presence of FGF-8 in adult tissues and cells including the reproductive tract, peripheral leukocytes, and hematopoietic cells [17-19]. Further functional studies are required to fully delineate the role of FGF-8 and its isoforms in the normal adult.
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