FGF-2, also known as basic FGF or bFGF, is the essential component in all embryonic and induced pluripotent stem cell media for maintenance of pluripotency. However, FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation. This fundamental biochemical instability, and therefore low half-life in culture media (<10 h), is an important contribution to the need for frequent media changes and challenges in improving homogeneity during stem cell proliferation and subsequent differentiation.
We have licensed thermostable (heat stable) FGF-2 (bFGF) technology – FGF2-G3 – from Enantis. This innovation was combined with our manufacture expertise to generate an animal-free thermostable (heat stable) FGF2-G3 protein to provide compatibility with stem cell culture process development and scale-up. Additionally, we offer both FGF2-G3 145aa (Qk052) and the 154aa original form (Qk053) so you can compare directly with the wild-type FGF-2 that you currently use.
If you would like to evaluate FGF2-G3, please contact us.