Thermostable FGF2-G3
for weekend-free stem cell culture
Thermostable FGF2-G3 is a hyperstable form of FGF-2 (bFGF), the essential component for the maintenance of pluripotency in stem cell media.
FGF-2, also known as basic FGF or bFGF, is the essential component in all embryonic and induced pluripotent stem cell media for the maintenance of pluripotency. However, FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation. This fundamental biochemical instability, and therefore low half-life in culture media (<10 h), is an important contribution to the need for frequent media changes and challenges in improving homogeneity during stem cell proliferation and subsequent differentiation.
We have licensed thermostable (heat stable) FGF-2 (bFGF) technology – FGF2-G3 – from Enantis. This innovation was combined with our manufacture expertise to generate an animal-free thermostable (heat stable) FGF2-G3 protein to provide compatibility with stem cell culture process development and scale-up. Additionally, we offer both FGF2-G3 145aa (Qk052) and the 154aa original form (Qk053) so you can compare directly with the wild-type FGF-2 that you currently use.
If you would like to evaluate FGF2-G3, please contact us.
FGF-2 (bFGF) proteins for media optimization
FGF-2 is an essential growth factor protein for many applications of stem cell culture. To allow you to fully optimize your protocol, we offer our FGF-2 in species-specific forms and in two lengths, 145 aa and 154 aa.
The 9 aa pro-segment of FGF-2 present in the 154 aa form is not required for biological activity and is thought to have roles in the localization of FGF-2 in vivo. We want to allow scientists to compare directly with their existing FGF-2, so we manufacture both forms in our wildtype FGF-2, and in our thermostable version, FGF2-G3.
Thermostable FGF2-G3
FGF2-G3 145 aa & 154 aa
FGF2-G3 (FGF2-STAB®) is a bioactive and high-quality thermostable (heat-stable) engineered form of FGF2 (bFGF).
FGF2-G3 supports high-quality, cost-effective cell culture, preventing the need for frequent media changes (stable for > 7 days in culture).
The patented FGF2-G3 technology combines protein manufacturing expertise from Qkine for weekend-free cell culture.
Wild-type FGF-2
FGF-2 145 aa & 154 aa
Human FGF2 /bFGF protein is highly bioactive and animal-derived component-free. FGF2 (bFGF) has been used for the culture, maintenance and differentiation of induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs).
FGF-2 is available in two forms, 145 aa and 154 aa, to allow you to fully optimize your media.
A brief history of FGF-2 in stem cell culture
Featured resources
Key papers
Thermostable FGF2-G3 for weekend-free stem cell culture
We are confident that the quality and bioactivity of our recombinant proteins will ensure that you achieve your research goals. Our dedicated team of stem cell specialists is available to answer any queries you may have and to give expert support when required.