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recombinant human EGF protein (Qk011)

Human EGF protein is a potent EGF-family growth factor used in many human and mouse organoid and stem cell culture systems including intestinal and tumor (cancer) organoid culture.

A pure 6.3 kDa biologically active mature human epidermal growth factor. Our recombinant EGF protein is manufactured to the highest purity in E. coli and extensively tested, making it ideal for use in chemically defined iPSC, ESC and organoid culture media.

Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.

1005001000
Clear

buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity human EGF protein (residues 971-1023, Uniprot: P01133)

  • 6.3 kDa

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • tumor organoid culture

bioactivity

Human EGF Qk011 protein bioactivity lot #011

EGF activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells.

Cells are treated (n=2) with a serial dilution of EGF for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.  EC50 = 29 pg/ml (4.6pM). Data from Qk011 batch #011.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

Human EGF Qk011 protein purity SDS-PAGE lot #011

EGF protein migrates as a single band at ~7 kDa in non-reducing (NR) and 19 kDa in reducing (R) conditions.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.  Data from Qk011 lot #011.

further quality assays

  • mass spectrometry: single species with expected mass

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

organoid
iPSC/ESC maintenance
neural
hepatocyte
pancreatic
endodermal
mesodermal
epithelial
mesenchymal
hematopoietic

protein background

Human epidermal growth factor (EGF) stimulates cell proliferation and differentiation. It is used extensively in induced-pluripotent stem cell (iPSC) and embryonic stem cell (ESC) culture systems for the successful expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages.

Recombinant EGF protein is a key component of many organoid media, along with other stem cell niche factors, R-spondin 1, Noggin or Gremlin, Wnt3a and FGF10. In addition, recombinant EGF is used for establishing and maintaining intestine, stomach, liver, pancreas, brain and cancer organoids.

Members of the human epidermal growth factor family are synthesized as type I transmembrane precursor proteins, often containing several EGF domains in the extracellular region. Proteolysis yields mature proteins that are released from the cell surface1. The mature form of human EGF protein comprises 53 amino acids2. Epidermal growth factor binds to high-affinity EGF receptors (EGFRs) and promotes receptor dimerization and clustering leading to activation of downstream signalling pathways, including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signalling3.

product documents

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for human EGF, please contact us info@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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