Human EGF Qk011 protein purity SDS-PAGE lot #011

Recombinant human EGF protein (Qk011)

Name: Recombinant human EGF protein (Qk011)
SKU: Qk011
Availability: InStock

Human EGF protein is a potent EGF-family growth factor used in many human and mouse organoid and stem cell culture systems including intestinal and tumor (cancer) organoid culture.

A pure 6.3 kDa biologically active mature human epidermal growth factor. Our recombinant EGF protein is manufactured to the highest purity in E. coli and extensively tested, making it ideal for use in chemically defined iPSC, ESC and organoid culture media.

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1mg may be despatched as 2 x 500µg

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Description

Summary

High purity human EGF protein (residues 971-1023, Uniprot: P01133)
>97%, by SDS-PAGE quantitative densitometry
6.3 kDa
Expressed in E. coli
Animal-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Handling and storage FAQ

Featured applications

Tumor organoid culture

Alternative protein names

Epidermal growth factor, Pro-epidermal growth factor, HOMG4, Beta-urogastrone, Urogastrone (URG)

Background references

1. Harris, R. C., Chung, E. & Coffey, R. J. EGF receptor ligands. Experimental cell research 284, 2–13 (2003).
2. Bell, G. I. et al. Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization. Nucleic acids research 14, 8427–8446 (1986).
3. Carpenter, G. & Cohen, S. Epidermal growth factor. Journal of biological chemistry 265, 7709–7712 (1990).

Species reactivity

Human

Species similarity:
Porcine – 85%
Mouse – 74%
Rat – 70%

Frequently used together

Recombinant human Noggin protein
Recombinant human R-spondin 1 protein
Recombinant human FGF10 protein

Bioactivity

EGF activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of EGF for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.

EC50 = 11.6 pM (72.8 pg/mL).

Data from Qk011 lot #10.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

EGF protein migrates as a single band at ~12 kDa in non-reducing (NR) and 9 kDa in reducing (R) conditions.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk011 lot #011.

Further quality assays

Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%

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Download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Human epidermal growth factor (EGF) stimulates cell proliferation and differentiation. It is used extensively in induced-pluripotent stem cell (iPSC) and embryonic stem cell (ESC) culture systems for the successful expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages.

Recombinant EGF protein is a key component of many organoid media, along with other stem cell niche factors, R-spondin 1, Noggin or Gremlin, Wnt3a and FGF10. In addition, recombinant EGF is used for establishing and maintaining intestine, stomach, liver, pancreas, brain and cancer organoids.

Members of the human epidermal growth factor family are synthesized as type I transmembrane precursor proteins, often containing several EGF domains in the extracellular region. Proteolysis yields mature proteins that are released from the cell surface¹. The mature form of human EGF protein comprises 53 amino acids². Epidermal growth factor binds to high-affinity EGF receptors (EGFRs) and promotes receptor dimerization and clustering leading to activation of downstream signalling pathways, including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signalling³.

Additional resources

Technote: EGF

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Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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