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Home/recombinant growth factor & cytokine, human, neural/recombinant human PDGF-BB protein (Qk044)

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recombinant human PDGF-BB protein (Qk044)

human PDGF-BB (platelet derived growth factor BB) protein is a homodimer of PDGF-B peptide chains. PDGF is a potent mitogen involved in embryogenesis.1

PDGF-BB is used in neuronal differentiation2 and treatment of periodontal and orthopedic bone defects and dermal wound healing.3

High purity 25 kDa disulfide–linked bioactive dimeric human PDGF-B protein. Animal-free (AF) and carrier protein-free.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

25501005001000
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buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity human PDGF-BB protein (Uniprot:P01127)

  • 25 kDa

  • expressed in E. coli. 

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • neuronal differentiation

GDGF, Glioma-derived growth factor, ODGF, Osteosarcoma-derived growth factor
  1. Andrae, Johanna, et al. ‘Role of Platelet-Derived Growth Factors in Physiology and Medicine’. Genes & Development, vol. 22, no. 10, May 2008, pp. 1276–312. doi:10.1101/gad.1653708.
  2. Kim, Jung Yeon, et al. ‘Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells’. Tissue Engineering and Regenerative Medicine, vol. 15, no. 2, Dec. 2017, pp. 195–209. doi:10.1007/s13770-017-0102-x.
  3. Tavelli, L., et al. ‘Recombinant Human Platelet–Derived Growth Factor: A Systematic Review of Clinical Findings in Oral Regenerative Procedures’. JDR Clinical & Translational Research, vol. 6, no. 2, Apr. 2021, pp. 161–73. doi:10.1177/2380084420921353.
  4. Demoulin, Jean-Baptiste, and Carmen P. Montano-Almendras. ‘Platelet-Derived Growth Factors and Their Receptors in Normal and Malignant Hematopoiesis’. American Journal of Blood Research, vol. 2, no. 1, Jan. 2012, pp. 44–56.
  5. Kardas, Grzegorz, et al. ‘Role of Platelet-Derived Growth Factor (PDGF) in Asthma as an Immunoregulatory Factor Mediating Airway Remodeling and Possible Pharmacological Target’. Frontiers in Pharmacology, vol. 0, 2020. doi:10.3389/fphar.2020.00047.
  6. Demoulin, Jean-Baptiste, and Ahmed Essaghir. ‘PDGF Receptor Signaling Networks in Normal and Cancer Cells’. Cytokine & Growth Factor Reviews, vol. 25, no. 3, June 2014, pp. 273–83. doi:10.1016/j.cytogfr.2014.03.003.

bioactivity

Human LIF Qk036 protein bioactivity lot #14293

PDGF-BB activity is determined by the proliferation of Balb/c 3T3 cells. Cells are treated in triplicate with a serial dilution of PDGF-BB for 3 days. Cell viability is measured using the CellTiter-Glo (Promega) luminescence assay. Data from Qk044 lot #104306. EC50 = 0.42 ng/ml (0.017 pM).

purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

PDGF-BB migrates as a single band at ~30 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R).  No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk044 batch #104306.

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak 

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial >95%

request CoA/animal-free certificate
download MSDS

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

Platelet derived growth factor (PDGF) is part of the PDGF/VEGR family. As the name suggests it is produced by platelets, and by several other cell types including endothelial cells, fibroblasts, vascular smooth muscle cells, osteoblasts, glia and neurons.4 In platelets, PDGF is stored in α-granules, and released in response to platelet activation, leading to stimulation of epithelial cells.

PDGFs are dimers of PDGF-A, -B, -C or -D polypeptide chains, arranged in homodimers (eg PDGF-AA and PDGF-BB) or heterodimers (eg PDGF-AB). There are two receptors for PDGFs, PDGFR-α and PDGFR-β. PDGFR-α is a receptor for all PDGF dimers apart from PDGF-DD, while PDGFR-β is a receptor for PDGF-BB and PDGF-DD.5 Activation of these receptors by PDGF leads to activation of downstream signalling pathways including PI3K, MAPK and STAT3 pathways.6 PDGF plays an important role in embryonic development, cell proliferation, cell migration and angiogenesis. PDGF is a potent mitogen for cells of mesenchymal origin such as fibroblasts, glial cells and vascular smooth muscle cells.

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback, please submit a review or contact our team support@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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