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Human PDGF-BB (platelet derived growth factor BB) protein is a homodimer of PDGF-B peptide chains. PDGF is a potent mitogen involved in embryogenesis.1
PDGF-BB is used in neuronal differentiation2 and treatment of periodontal and orthopedic bone defects and dermal wound healing.3
High purity 25 kDa disulfide–linked bioactive dimeric human PDGF-B protein. Animal-free (AF) and carrier protein-free.
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1mg may be despatched as 2 x 500µg
Buy online with secure credit card or purchase order or email email@example.com
Bulk and stock reservation available
High purity human PDGF-BB protein (Uniprot:P01127)
>97%, by SDS-PAGE quantitative densitometry
Expressed in E. coli.
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Recombinant human PDGF-AA protein
PDGF-BB activity is determined by the proliferation of Balb/c 3T3 cells. Cells are treated in triplicate with a serial dilution of PDGF-BB for 3 days. Cell viability is
PDGF-BB activity is determined using the CellTiter-Glo (Promega) luminescence assay in Balb/c 3T3 cells. Cells are treated in triplicate with a serial dilution of PDGF-BB for 3 days. Cell viability is measured and normalized.
EC50 = 14.9 pM (0.37 ng/mL).
Data from Qk044 lot #104306.
PDGF-BB migrates as a single band at ~30 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk044 batch #104306.
Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial >95%
We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Platelet derived growth factor (PDGF) is part of the PDGF/VEGR family. As the name suggests it is produced by platelets, and by several other cell types including endothelial cells, fibroblasts, vascular smooth muscle cells, osteoblasts, glia and neurons.4 In platelets, PDGF is stored in α-granules, and released in response to platelet activation, leading to stimulation of epithelial cells.
PDGFs are dimers of PDGF-A, -B, -C or -D polypeptide chains, arranged in homodimers (eg PDGF-AA and PDGF-BB) or heterodimers (eg PDGF-AB). There are two receptors for PDGFs, PDGFR-α and PDGFR-β. PDGFR-α is a receptor for all PDGF dimers apart from PDGF-DD, while PDGFR-β is a receptor for PDGF-BB and PDGF-DD.5 Activation of these receptors by PDGF leads to activation of downstream signalling pathways including PI3K, MAPK and STAT3 pathways.6 PDGF plays an important role in embryonic development, cell proliferation, cell migration and angiogenesis. PDGF is a potent mitogen for cells of mesenchymal origin such as fibroblasts, glial cells and vascular smooth muscle cells.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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