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Human FGF4 protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation. FGF4 is an important component of cardiac, intestinal and other organoid culture media.
High purity and bioactivity 14 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 4, animal-derived component free (ACDF) and carrier-protein free (CF).
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1mg may be despatched as 2 x 500µg
high purity human FGF4 protein (residues 79-206, Uniprot: P08620)
expressed in E. coli
manufactured in our Cambridge, UK laboratories
frozen in PBS (1 mg/ml)
prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC
hindgut organoids from iPSC-derived human posterior gut endoderm cells7
neural stem cell proliferation and neuronal differentiation
FGF4 induces cell proliferation of iPSC cells (Stemnovate iPSC1 line). Data from Qk004 lot #010
Recombinant human FGF4 protein migrates as a single band at 14 kDa in non-reducing conditions (NR) and upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.
Data from Qk004 lot #010
further quality assays
mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Fibroblast growth factor 4 (FGF4) is a member of the FGF superfamily with a physiological role in the regulation of proliferation and differentiation in embryonic stem cells and tissue stem cells1-3.
Recombinant human FGF4 protein promotes neural stem cell proliferation and neuronal differentiation in the postnatal brain4, increases the proliferation rate of human adult bone-marrow derived mesenchymal stem cells5 and supports the maintenance, proliferation and self-renewal properties of human embryonic stem cells6.
Synergism between FGF4 and WNT signalling acts to form hindgut organoids from iPSC-derived human posterior gut endoderm cells7 and recombinant human FGF4 protein is used to mimic embryonic intestinal development during directed differentiation in culture of pluripotent stem cells into intestinal organoids.
FGF-4 induces cell proliferation of iPSC cells (Stemnovate iPSC1 line)
FGF-4 protein induces proliferation of HEK293T cells.
Human embryonic kidney cell line 293T (HEK293T) was cultured in serum-free media containing 100ng/ml hFGF2. The number of viable metabolically active cells was detected by ATP measurement using a bioluminescent reaction (n=3; data show mean ± SEM). Data provided by Stemnovate Ltd, Cambridge, UK.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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