Human FGF-4 protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation. FGF-4 is an important component of cardiac, intestinal and other organoid culture media.
High purity and bioactivity 14 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 4, animal-derived component free (ADCF) and carrier-protein free.
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1mg will be despatched as 2 x 500µg
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High purity human FGF4 protein (residues 79-206, Uniprot: P08620)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal-derived component free (ADCF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from PBS
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Hindgut organoids from iPSC-derived human posterior gut endoderm cells7
Neural stem cell proliferation and neuronal differentiation
mouse – 91%
porcine – 91%
bovine – 90%
rat – 80%
Recombinant human noggin protein
Recombinant human BMP4 protein
Recombinant human FGF10 protein
FGF-4 activity is determined using the firefly luciferase reporter assay in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-4. Firefly luciferase activity is measured and normalized.
EC50 = 306.5 pM (4.3 ng/mL).
Data from Qk004 lot #104330.
Recombinant human FGF4 protein migrates as a single band at 14 kDa in non-reducing conditions (NR) and upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.
Data from Qk004 lot #010
Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Fibroblast growth factor 4 (FGF4) is a member of the FGF superfamily with a physiological role in the regulation of proliferation and differentiation in embryonic stem cells and tissue stem cells1-3.
Recombinant human FGF4 protein promotes neural stem cell proliferation and neuronal differentiation in the postnatal brain4, increases the proliferation rate of human adult bone-marrow derived mesenchymal stem cells5 and supports the maintenance, proliferation and self-renewal properties of human embryonic stem cells6.
Synergism between FGF4 and WNT signalling acts to form hindgut organoids from iPSC-derived human posterior gut endoderm cells7 and recombinant human FGF4 protein is used to mimic embryonic intestinal development during directed differentiation in culture of pluripotent stem cells into intestinal organoids.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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