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recombinant human FGF-4 protein (Qk004)

Human FGF-4 protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation.  FGF-4 is an important component of cardiac, intestinal and other organoid culture media.

High purity and bioactivity 14 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 4, animal-derived component free (ACDF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

501005001000
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buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity human FGF4 protein (residues 79-206, Uniprot: P08620)

  • 14 kDa

  • expressed in E. coli

  • carrier protein-free

  • manufactured in our Cambridge, UK laboratories

  • frozen in PBS (1 mg/ml)

  • prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • hindgut organoids from iPSC-derived human posterior gut endoderm cells7

  • neural stem cell proliferation and neuronal differentiation

Fibroblast Growth Factor-4, Kaposi’s sarcoma-associated FGF, k-FGF, Heparin secretory-transforming protein 1 HST-1, Transforming protein KS3, Heparin-binding growth factor 4, HBGF-4
human

species similarity:
mouse – 91%
porcine – 91%
bovine – 90%
rat – 80%

bioactivity

Qk004 FGF4-lot-#010-proliferation of iPSC

FGF4 induces cell proliferation of iPSC cells (Stemnovate iPSC1 line).  Data from Qk004 lot #010

purity

human-FGF-4-Qk004-protein-purity-lot-010

Recombinant human FGF4 protein migrates as a single band at 14 kDa in non-reducing conditions (NR) and upon reduction (R). No contaminating protein bands are visible.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Data from Qk004 lot #010

further quality assays

  • mass spectrometry: single species with expected mass

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

organoid
iPSC/ESC maintenance
neural
hepatocyte
pancreatic
endodermal
mesodermal
epithelial
mesenchymal
hematopoietic

protein background

Fibroblast growth factor 4 (FGF4) is a member of the FGF superfamily with a physiological role in the regulation of proliferation and differentiation in embryonic stem cells and tissue stem cells1-3.

Recombinant human FGF4 protein promotes neural stem cell proliferation and neuronal differentiation in the postnatal brain4, increases the proliferation rate of human adult bone-marrow derived mesenchymal stem cells5 and supports the maintenance, proliferation and self-renewal properties of human embryonic stem cells6.

Synergism between FGF4 and WNT signalling acts to form hindgut organoids from iPSC-derived human posterior gut endoderm cells7 and recombinant human FGF4 protein is used to mimic embryonic intestinal development during directed differentiation in culture of pluripotent stem cells into intestinal organoids.

product documents

FGF-4 induces cell proliferation of iPSC cells (Stemnovate iPSC1 line)

FGF4 induces proliferation of iPSC
IPSC (Stemnovate iPSC1) were cultured in serum-free media containing 100ng/ml hFGF4. The number of viable metabolically active cells was detected by ATP measurement using a bioluminescent reaction (n=3; data show mean ± SEM). No media change was performed on day 2 leading to reduced viable cell numbers on day 3. Data provided by Stemnovate Ltd, Cambridge, UK.

FGF-4 protein induces proliferation of HEK293T cells.

Qk004 FGF4-lot-#010-proliferation of HEK293T

Human embryonic kidney cell line 293T (HEK293T) was cultured in serum-free media containing 100ng/ml hFGF2. The number of viable metabolically active cells was detected by ATP measurement using a bioluminescent reaction (n=3; data show mean ± SEM). Data provided by Stemnovate Ltd, Cambridge, UK.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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