Recombinant human GDNF protein (Qk051)
Human Glial cell line-derived neurotrophic factor (GDNF) protein supports the maintenance and differentiation of numerous types of neurons including dopaminergic neurons. GDNF is used to differentiate human pluripotent stem cell into neurons1 and in pluripotent stem cell derived brain organoids.2
A bioactive 30 kDa homodimer, our recombinant GDNF protein is highly pure and animal-free (AOF).
GDNF activity is determined using a SH-SY5Y cell proliferation assay. Cells were incubated with different concentrations of GDNF in the presence of retinoic acid and recombinant GFR α1 for 3 days before viable cell measurement using an MTS assay. Data are n=2.
EC50 = 18 ng/ml
Data from Qk051 batch #104372.
GDNF migrates as a single band at 30 kDa in non-reducing (NR) conditions and 15 kDa upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk051 batch #104372.
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Comparison with other suppliers
Qkine GDNF (Qk051) bioactivity was compared with an alternative supplier (Supplier 1) using a SH-SY5Y cell proliferation assay (as above). Qkine GDNF was found to have higher bioactivity than Supplier 1 GDNF. Qk051 EC50 = 18 ng/ml, Supplier 1 EC50 = 26 ng/ml, P = 0.018
Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the GDNF family of ligands (GFL). The GFLs act as biologically active homodimers that signal through the transmembrane receptor tyrosine kinase REarranged during Transfection (RET). GFL activation of RET is dependent upon co-receptors, namely the 4 GDNF Family Receptor α (GFRα1-4). GFL signalling specificity arises from the preferentially binding of each ligand to one of the 4 GFRα receptors; GDNF preferentially binds GFRα1.3
GFLs play roles in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration.4 GDNF is best known for its role in promoting survival and differentiation of midbrain dopaminergic neurons both in vivo and in vitro,5 and has been proposed as a treatment for numerous neurological conditions including Parkinsons diseases, motor neuron disease, drug addiction, and alcoholism.4
GDNF, together with Human Recombinant BDNF (Qk050), can be used to differentiate human pluripotent stem cell into neurons1 and in pluripotent stem cell derived cortical organoids.2
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
Very happy with this product. My neuronal cultures look very healthy and there has been no difference in morphology or mechanism of the neurons since switching from a more expensive competitive brand. Our lab will be continuing to purchase.