UK +44 (0)1223 491486      USA toll free 1-866 877 2185

Search
Generic filters
Home/recombinant growth factor & cytokine, human, neural, neurotrophin, organoid/Recombinant human GDNF protein (Qk051)

Currency

GBP: £

Recombinant human GDNF protein (Qk051)

Human Glial cell line-derived neurotrophic factor (GDNF) protein supports the maintenance and differentiation of numerous types of neurons including dopaminergic neurons. GDNF is used to differentiate human pluripotent stem cell into neurons1 and in pluripotent stem cell derived brain organoids.2

A bioactive 30 kDa homodimer, our recombinant GDNF protein is highly pure and animal-free (AOF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

25501005001000
Clear

buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

Summary

  • High purity GDNF (Uniprot: P39905)

  • 15.1 kDa monomer, 30.1 kDa dimer

  • Expressed in E. coli

  • Animal-free (AOF) and carrier protein-free

  • Manufactured in Cambridge, UK

  • Lyophilized from acetonitrile, TFA

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

Handling and storage FAQ

Featured applications

  • Differentiation of midbrain dopaminergic neurons

Astrocyte-derived trophic factor, ATF, ATF1, ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1-GDNF; HSCR3

Bioactivity

Zebrafish FGF2 / bFGF Qk002 protein bioactivity lot #011

GDNF activity is determined using a SH-SY5Y cell proliferation assay. Cells were incubated with different concentrations of GDNF in the presence of retinoic acid and recombinant GFR α1 for 3 days before viable cell measurement using an MTS assay. Data are n=2. Assay performed by SBH Sciences.

EC50 = 18 ng/ml

Data from Qk051 batch #104372.

Purity

Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

GDNF migrates as a single band at 30 kDa in non-reducing (NR) conditions and 15 kDa upon reduction (R).  No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk051 batch #104372.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial:  >95%

Request CoA/animal-free certificate

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Comparison with other suppliers

Qkine GDNF (Qk051) bioactivity was compared with an alternative supplier (Supplier 1) using a SH-SY5Y cell proliferation assay (as above). Qkine GDNF was found to have higher bioactivity than Supplier 1 GDNF. Qk051 EC50 = 18 ng/ml, Supplier 1 EC50 = 26 ng/ml, P = 0.018

Protein background

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the GDNF family of ligands (GFL). The GFLs act as biologically active homodimers that signal through the transmembrane receptor tyrosine kinase REarranged during Transfection (RET). GFL activation of RET is dependent upon co-receptors, namely the 4 GDNF Family Receptor α (GFRα1-4). GFL signalling specificity arises from the preferentially binding of each ligand to one of the 4 GFRα receptors; GDNF preferentially binds GFRα1.3

GFLs play roles in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration.4 GDNF is best known for its role in promoting survival and differentiation of midbrain dopaminergic neurons both in vivo and in vitro,5 and has been proposed as a treatment for numerous neurological conditions including Parkinsons diseases, motor neuron disease, drug addiction, and alcoholism.4

GDNF, together with Human Recombinant BDNF (Qk050), can be used to differentiate human pluripotent stem cell into neurons1 and in pluripotent stem cell derived cortical organoids.2

  1. McComish, Sarah F., and Maeve A. Caldwell. ‘Generation of Defined Neural Populations from Pluripotent Stem Cells’. Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 373, no. 1750, July 2018, p. 20170214. PubMed Central, https://doi.org/10.1098/rstb.2017.0214.
  2. Jacob, Fadi, et al. ‘Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium’. Cell Stem Cell, vol. 27, no. 6, Dec. 2020, pp. 937-950.e9. cell.com, https://doi.org/10.1016/j.stem.2020.09.016.
  3. ‘Signalling and Receptors of GDNF’. University of Helsinki, 3 Nov. 2017, https://www2.helsinki.fi/en/researchgroups/neurotrophic-factors-and-regeneration/research/signalling-and-receptors-of-gdnf.
  4. Hsieh, Joseph C., and Richard D. Penn. ‘Chapter 44 – Infusion Therapy for Movement Disorders’. Neuromodulation, edited by Elliot S. Krames et al., Academic Press, 2009, pp. 561–70. ScienceDirect, https://doi.org/10.1016/B978-0-12-374248-3.00045-8.
  5. Granholm, Ann-Charlotte, et al. ‘Glial Cell Line-Derived Neurotrophic Factor Is Essential for Postnatal Survival of Midbrain Dopamine Neurons’. Journal of Neuroscience, vol. 20, no. 9, May 2000, pp. 3182–90. jneurosci.org, https://doi.org/10.1523/JNEUROSCI.20-09-03182.2000.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

Share your thoughts!

Let us know what you think...

What others are saying

There are no contributions yet.

×

Login

Register

Your personal data will be used to support your experience throughout this website, to manage access to your account, and for other purposes described in our privacy policy.

Continue as a Guest

Don't have an account? Sign Up

Title

Go to Top