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Recombinant human GDNF protein (Qk051)
Human Glial cell line-derived neurotrophic factor (GDNF) protein supports the maintenance and differentiation of numerous types of neurons including dopaminergic neurons. GDNF is used to differentiate human pluripotent stem cell into neurons1 and in pluripotent stem cell derived brain organoids.2
A bioactive 30 kDa homodimer, our recombinant GDNF protein is highly pure and animal-free (AOF).
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1mg may be despatched as 2 x 500µg
Buy online with secure credit card or purchase order or email orders@qkine.com
Bulk and stock reservation available
Summary
High purity GDNF (Uniprot: P39905)
15.1 kDa monomer, 30.1 kDa dimer
Expressed in E. coli
Animal-free (AOF) and carrier protein-free
Manufactured in Cambridge, UK
Lyophilized from acetonitrile, TFA
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC
Featured applications
Differentiation of midbrain dopaminergic neurons
Astrocyte-derived trophic factor, ATF, ATF1, ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1-GDNF; HSCR3
Human
Bioactivity
GDNF activity is determined using a SH-SY5Y cell proliferation assay. Cells were incubated with different concentrations of GDNF in the presence of retinoic acid and recombinant GFR α1 for 3 days before viable cell measurement using an MTS assay. Data are n=2.
EC50 = 18 ng/ml
Data from Qk051 batch #104372.
Purity
GDNF migrates as a single band at 30 kDa in non-reducing (NR) conditions and 15 kDa upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk051 batch #104372.
Further quality assays
Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Comparison with other suppliers
Qkine GDNF (Qk051) bioactivity was compared with an alternative supplier (Supplier 1) using a SH-SY5Y cell proliferation assay (as above). Qkine GDNF was found to have higher bioactivity than Supplier 1 GDNF. Qk051 EC50 = 18 ng/ml, Supplier 1 EC50 = 26 ng/ml, P = 0.018
Protein background
Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the GDNF family of ligands (GFL). The GFLs act as biologically active homodimers that signal through the transmembrane receptor tyrosine kinase REarranged during Transfection (RET). GFL activation of RET is dependent upon co-receptors, namely the 4 GDNF Family Receptor α (GFRα1-4). GFL signalling specificity arises from the preferentially binding of each ligand to one of the 4 GFRα receptors; GDNF preferentially binds GFRα1.3
GFLs play roles in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration.4 GDNF is best known for its role in promoting survival and differentiation of midbrain dopaminergic neurons both in vivo and in vitro,5 and has been proposed as a treatment for numerous neurological conditions including Parkinsons diseases, motor neuron disease, drug addiction, and alcoholism.4
GDNF, together with Human Recombinant BDNF (Qk050), can be used to differentiate human pluripotent stem cell into neurons1 and in pluripotent stem cell derived cortical organoids.2
- McComish, Sarah F., and Maeve A. Caldwell. ‘Generation of Defined Neural Populations from Pluripotent Stem Cells’. Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 373, no. 1750, July 2018, p. 20170214. PubMed Central, https://doi.org/10.1098/rstb.2017.0214.
- Jacob, Fadi, et al. ‘Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium’. Cell Stem Cell, vol. 27, no. 6, Dec. 2020, pp. 937-950.e9. cell.com, https://doi.org/10.1016/j.stem.2020.09.016.
- ‘Signalling and Receptors of GDNF’. University of Helsinki, 3 Nov. 2017, https://www2.helsinki.fi/en/researchgroups/neurotrophic-factors-and-regeneration/research/signalling-and-receptors-of-gdnf.
- Hsieh, Joseph C., and Richard D. Penn. ‘Chapter 44 – Infusion Therapy for Movement Disorders’. Neuromodulation, edited by Elliot S. Krames et al., Academic Press, 2009, pp. 561–70. ScienceDirect, https://doi.org/10.1016/B978-0-12-374248-3.00045-8.
- Granholm, Ann-Charlotte, et al. ‘Glial Cell Line-Derived Neurotrophic Factor Is Essential for Postnatal Survival of Midbrain Dopamine Neurons’. Journal of Neuroscience, vol. 20, no. 9, May 2000, pp. 3182–90. jneurosci.org, https://doi.org/10.1523/JNEUROSCI.20-09-03182.2000.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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What others are saying
Great product
hh495 –
Very happy with this product. My neuronal cultures look very healthy and there has been no difference in morphology or mechanism of the neurons since switching from a more expensive competitive brand. Our lab will be continuing to purchase.
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