Recombinant human/bovine/porcine NRG-1 protein (Qk045)

Name: Recombinant human/bovine/porcine NRG-1 protein (Qk045)
SKU: Qk045
Availability: InStock

Human NRG-1 protein (Neuregulin 1) is frequently used in the maintenance of human pluripotent stem cells. In addition to its widespread use in stem cell culture media, NRG-1 (also known as Heregulin-β1 , HRG-1) has essential roles in vivo including in nervous system, cardiac, and mammary gland development; cancer biology and neurological disorders.

7.5 kDa highly pure, bioactive domain of human NRG-1, comprised of the β isoform of the EGF-like domain of NRG-1 (HRG1-B1). This NRG-1 protein monomer is animal-free (AF) and carrier-protein free (CF).

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1mg will be despatched as 2 x 500µg

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Description

Summary

High purity human NRG-1 protein (Uniprot:Q02297)
>98%, by SDS-PAGE quantitative densitometry
7.5 kDa
Expressed in E. coli.
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Handling and storage FAQ

Featured applications

Maintenance of pluripotency in human iPSC

Alternative protein names

Neuregulin1 (NRG1-β1), Neu differentiation factor (rat), Heregulin, HRG, HRG1-β1, Breast cancer cell differentiation factor p45, ARIA (Acetylcholine, Receptor Inducing Activity), glial growth factor

Background references

Mei, Lin, and Wen-Cheng Xiong. ‘Neuregulin 1 in Neural Development, Synaptic Plasticity and Schizophrenia’. Nature Reviews Neuroscience, vol. 9, no. 6, June 2008, pp. 437–52. www.nature.com, https://doi.org/10.1038/nrn2392.
Breuleux, M. ‘Role of Heregulin in Human Cancer’. Cellular and Molecular Life Sciences, vol. 64, no. 18, Sept. 2007, pp. 2358–77. Springer Link, https://doi.org/10.1007/s00018-007-7120-0.
Hobbs, Stuart S., et al. ‘Neuregulin Isoforms Exhibit Distinct Patterns of ErbB Family Receptor Activation’. Oncogene, vol. 21, no. 55, Dec. 2002, pp. 8442–52. www.nature.com, https://doi.org/10.1038/sj.onc.1205960.
Shi, Liang, and Clare M. Bergson. ‘Neuregulin 1: An Intriguing Therapeutic Target for Neurodevelopmental Disorders’. Translational Psychiatry, vol. 10, no. 1, June 2020, pp. 1–11. www.nature.com, https://doi.org/10.1038/s41398-020-00868-5.
Berdiel-Acer, Mireia, et al. ‘Stromal NRG1 in Luminal Breast Cancer Defines Pro-Fibrotic and Migratory Cancer-Associated Fibroblasts’. Oncogene, vol. 40, no. 15, Apr. 2021, pp. 2651–66. www.nature.com, https://doi.org/10.1038/s41388-021-01719-3.

Species reactivity

Human
Porcine (pig)
Bovine (cow)
Mouse (expected, 98.5% identity)

Frequently used together

Recombinant human activin A protein
Recombinant zebrafish FGF2 protein
Recombinant human FGF2 145aa protein
Recombinant human IGF-1 LR3 protein
Recombinant human TGF-β1 PLUS protein
Recombinant human NRG1 protein

Bioactivity

NRG-1 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. Cells are treated in triplicate with a serial dilution of NRG-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.

EC50 = 37.9 pM (0.28 ng/mL).

Data from Qk045 lot #104317.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

NRG-1 migrates as a single band at 7.5 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk045 batch #104317.

Further quality assays

Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial >95%

Request CoA/animal-free certificate

Download MSDS

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Neuregulin 1 (NRG-1) is a member of the neuregulin family (along with NRG 2-4). Each member of this family is encoded by an individual gene. Alternative splicing and different promoter utilisation results in a large number of isoforms for each member of the protein family – for example, NRG-1, the best studied member, generates six types of protein (I–VI) and at least 31 isoforms.¹ These isoforms have different tissue distribution, potency, receptor specificity and biological functions.²

All NRG-1 isoforms contain the bioactive EGF-like domain, this EGF-like domain has two isoforms: alpha and beta. These differ in their receptor affinity; both the alpha and beta isoforms are ligands for the receptors ErbB3 (HER3) and ErbB4 (HER4), however the NRG-1 beta isoform has a higher affinity for the ErbB4 receptor than the alpha isoform.³ This protein (Qk045) consists of the β isoform of the EGF domain of NRG-1 protein.

NRG-1 has an essential role in cardiac and neuronal development, and is implicated in disorders such as schizophrenia,⁴ and numerous cancers, for example breast cancer where NRG-1 promotes the proliferation and migration of breast cancer cells.⁵ In cell culture, NRG-1 is frequently used in maintenance media for human pluripotent stem cells and other cells.

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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