Recombinant human NRG-1 protein (Qk045) Price range: £175.00 through £880.00View product
Recombinant human NRG-1 protein (Qk045)
Price range: £175.00 through £880.00
Human NRG-1 protein (neuregulin 1) is frequently used in the maintenance of human pluripotent stem cells.  In addition to its widespread use in stem cell culture media, NRG-1 (also known as Heregulin-β1 , HRG-1) has essential roles in vivo including in nervous system, cardiac, and mammary gland development; cancer biology and neurological disorders.
Qkine NRG-1 is the 7.5 kDa highly pure, bioactive domain of human NRG-1, comprised of the β isoform of the EGF-like domain of NRG-1 (HRG1-B1). This NRG-1 protein monomer is animal origin-free (AOF) and carrier-protein free (CF).
This protein is also available as GMP compliant Cell Therapy Grade, to enquire email support@qkine.com.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £175.00 through £880.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human NRG-1 protein (Uniprot: Q02297)
- 7.5 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Maintenance of pluripotency in iPSC
Frequently used together
- Recombinant human TGF-β1 PLUS™ protein (Qk010)
- Recombinant human IGF-1 protein (Qk047)
- Recombinant human IGF-1 LR3 protein (Qk041)
- Recombinant human activin A protein (Qk001)
- Recombinant porcine EGF protein (Qk064)
- Recombinant bovine PDGF-BB protein (Qk079)
- Recombinant human activin A PLUSâ„¢ protein (Qk005)
Related discovery kits
Alternative proteins
NRG-1 activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of NRG-1 for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 37.9 pM (0.28 ng/ml). Data from Qk045 lot #104317.
NRG-1 migrates as a single band at 7.5 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk045 batch #104317.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine NRG-1 was as bioactive as NRG-1 from an alternative supplier. Recombinant NRG-1 activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of Qkine NRG-1 (Qk045, green) or alternative supplier NRG-1 (Supplier B, black) for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity.
Protein background
Neuregulin 1 (NRG-1) is a member of the neuregulin family (along with NRG 2-4). Each member of this family is encoded by an individual gene. Alternative splicing and different promoter utilisation results in a large number of isoforms for each member of the protein family – for example, NRG-1, the best studied member, generates six types of protein (I–VI) and at least 31 isoforms [1]. These isoforms have different tissue distribution, potency, receptor specificity and biological functions [2].
All NRG-1 isoforms contain the bioactive EGF-like domain, this EGF-like domain has two isoforms: alpha and beta. These differ in their receptor affinity; both the alpha and beta isoforms are ligands for the receptors ErbB3 (HER3) and ErbB4 (HER4), however the NRG-1 beta isoform has a higher affinity for the ErbB4 receptor than the alpha isoform [3]. This protein (Qk045) consists of the β isoform of the EGF domain of NRG-1 protein.
NRG-1 has an essential role in cardiac and neuronal development, and is implicated in disorders such as schizophrenia [4], and numerous cancers, for example breast cancer where NRG-1 promotes the proliferation and migration of breast cancer cells [5]. In cell culture, NRG-1 is frequently used in maintenance media for human pluripotent stem cells and other cells.
Publications using Recombinant human NRG-1 protein (Qk045)
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Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture
Truszkowski L, Bottini S, Bianchi SÂ et al.Â
DOI: doi.org/10.12688/openreseurope.18245.2 -
Animal proteins from stem cells as an alternative to reduce the ecological and climate impact of animal farming
Bottini S (Thesis)
DOI: Thesis
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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