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Recombinant human KGF (FGF-7) protein (Qk046)

Human KGF protein (FGF-7)  is a member of the FGF family secreted by mesenchymal cells and has a paracrine effect on neighbouring epithelial cells.1 KGF protein is used in the culture of lung, breast, liver, and bladder organoids.2 In vivo, KGF is involved in wound healing and development of kidney, thymus, and hippocampus.1

High purity and bioactivity 19 kDa human FGF-7 / KGF protein, animal-origin free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1000µg will be despatched as 2 x 500µg

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  • High purity FGF-7 / KGF protein (Uniprot: P21781)

  • >98%, by SDS-PAGE quantitative densitometry

  • 19 kDa

  • Expressed in E. coli

  • Animal-free (AOF) and carrier protein-free

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from PBS

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

Featured applications

  • Airway, mammary, liver hepatocyte, and urothelium organoids

Fibroblast growth factor 7, Keratinocyte growth factor, KGF, Keratinocyte growth factor 1, KGF1 HBGF-7, Heparin-binding growth factor 7

  1. Zinkle, Allen, and Mohammadi, Moosa. ‘Structural Biology of the FGF7 Subfamily’. Frontiers in Genetics, vol. 10, 2019. Frontiers, doi:10.3389/fgene.2019.00102.
  2. Fujii, Masayuki, and Sato, Toshiro. ‘Somatic Cell-Derived Organoids as Prototypes of Human Epithelial Tissues and Diseases’. Nature Materials, vol. 20, no. 2, Feb. 2021, pp. 156–69. doi:10.1038/s41563-020-0754-0.
  3. Werner, Sabine, and Grose, Richard. ‘Regulation of Wound Healing by Growth Factors and Cytokines’. Physiological Reviews, vol. 83, no. 3, July 2003, pp. 835–70. doi:10.1152/physrev.2003.83.3.835
  4. Erdag, Gulsun, et al. ‘FGF-7 Expression Enhances the Performance of Bioengineered Skin’. Molecular Therapy, vol. 10, no. 1, July 2004, pp. 76–85. doi:10.1016/j.ymthe.2004.04.013.

Mature human FGF7, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF


Zebrafish FGF2 / bFGF Qk002 protein bioactivity lot #011

FGF-7 (KGF) activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. Cells are treated in triplicate with a serial dilution of KGF for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.

EC50 = 92 pM (1.75 ng/mL).

Data from Qk046 lot #104314.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340


Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

KGF protein migrates as a monomer at 19 kDa and a dimer at 38 kDa  in non-reducing (NR) conditions and 19 kDa upon reduction (R).  No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk046 lot #104314

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

FGF-7 KGF Supplier comparison

Comparison with other suppliers

KGF (FGF-7) from Qkine (Qk046) and a major supplier have equivalent bioactivity. Qk046 KGF (FGF-7) lot #104425 and KGF from another supplier were compared in the same assay plate with EC50s of 2.0 ng/ml and 3.7 ng/ml respectively. We were really pleased with how happy the cells were with our KGF and look forward to hearing feedback from customers!

Kerry, Qkine senior scientist

Protein background

Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is part of the large FGF family, and, alongside FGF-3, FGF-10, and FGF-22, is a member of the FGF-7 subfamily. This subfamily is secreted exclusively by the mesenchyme, and specifically activates the “b” isoforms of FGFR1 (FGFR1b) and FGFR2 (FGFR2b) present in the overlying epithelium1, of these receptors KGF acts through the IIIb splice form of FGFR2. KGF is required for the development of the kidney, thymus, and hippocampus.1 KGF also plays an import role in wound healing,3 a role exemplified in studies on bioengineered skin.KGF protein has been used in generation of many organoids including lung, breast, liver and bladder 2

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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  1. One person found this helpful
    Dylan Liabeuf

    liver organoids

    Dylan Liabeuf

    Thanks to the high purity and quality of Qkine’ proteins (hu-FGF7), we were able to maintain selected liver cells derived from adult patient specimens as organoids over time and passage them.

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  2. Question


    Where are references 1, 2, 3 and 4 referred to in the protein background above?

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    • Mairi Shepherd

      Mairi Shepherd

      The references can be found near the top of the page, in the ‘background references’ toggle, next to the summary table

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