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Human TGF-β3 protein is a member of the Transforming Growth Factor family, a family involved in regulating cell survival, proliferation and differentiation.1 TGF-β3 is used in human pluripotent stem cell maintenance medias, such as B8 media.2
High purity and bioactivity for reproducible results. Human TGF-β3 protein dimer, animal-origin free (AOF) and carrier-protein free (CF).
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1mg may be despatched as 2 x 500µg
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High purity TGF-β3 (Uniprot: P10600)
>97%, by SDS-PAGE quantitative densitometry
12.7 kDa monomer, 25.4 kDa dimer
Expressed in E. coli
Animal-free (AOF) and carrier protein-free
Manufactured in Cambridge, UK
Lyophilized from acetonitrile, TFA
Resuspend in 10mM HCl (Reconstitution solution A) at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at – 20°C or – 80°C
Human pluripotent stem cell maintenance
Transforming growth factor beta-3, TGF-b3, TGFB3, prepro-transforming growth factor beta-3, arrhythmogenic right ventricular dysplasia 1 (ARVD, ARVD1)
Recombinant human FGF-2 protein
Recombinant human FGF2-G3 protein
Recombinant human NRG1 protein
Recombinant human IGF-1 LR3 protein
TGF-β3 activity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. EC50 = 50 pg/ml.
Bioactivity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. Cells are treated (in triplicate) with a serial dilution of TGF-β3 for 6 hours. Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity. Data from Qk054 batch #104369.
TGF-β3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk054 batch #104369.
Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine TGF-β3 (Qk054) bioactivity was compared with an alternative supplier (Supplier 1) using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells (as above). Qkine TGF-β3 and Supplier 1 were found to have equivalent bioactivity, Qk054 EC50 = 50 pg/ml, Supplier 1 EC50 = 67 pg/ml (no significant difference).
Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors.3
The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation.1 TGF-β3 has isoform-specific roles in embryonic palate fusion4 and wound healing.5
In culture, TGF-β3 is often used in human pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University.2 In the development of B8 media, Kuo et al.2 proved that while TGF-β1 can maintain iPSCs at 2ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1ng/ml, making it a cost-effective alternative to TGF-β1.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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