Recombinant human TGF β3 protein (Qk054)
Human TGF β3 protein is a member of the Transforming Growth Factor family, a family involved in regulating cell survival, proliferation and differentiation.1 TGF β3 is used in human pluripotent stem cell maintenance medias, such as B8 media.2
High purity and bioactivity for reproducible results. Human TGF β3 protein dimer, animal-origin free (AOF) and carrier-protein free (CF).
TGF β3 activity is determined using a TGF β3 responsive firefly luciferase reporter in HEK293T cells. EC50 = 50 pg/ml.
Bioactivity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. Cells are treated (in triplicate) with a serial dilution of TGF-β3 for 6 hours. Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity. Data from Qk054 batch #104369.
TGF β3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk054 batch #104369.
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Comparison with other suppliers
Qkine TGF-β3 (Qk054) bioactivity was compared with an alternative supplier (Supplier 1) using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells (as above). Qkine TGF-β3 and Supplier 1 were found to have equivalent bioactivity, Qk054 EC50 = 50 pg/ml, Supplier 1 EC50 = 67 pg/ml (no significant difference).
Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors.3
The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation.1 TGF-β3 has isoform-specific roles in embryonic palate fusion4 and wound healing.5
In culture, TGF-β3 is often used in human pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University.2 In the development of B8 media, Kuo et al.2 proved that while TGF-β1 can maintain iPSCs at 2ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1ng/ml, making it a cost-effective alternative to TGF-β1.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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