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Home/human, iPSC / ESC maintenance, TGF beta family/Recombinant human TGF-β3 protein (Qk054)

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Recombinant human TGF-β3 protein (Qk054)

Human TGF-β3 protein is a member of the Transforming Growth Factor family, a family involved in regulating cell survival, proliferation and differentiation.1 TGF-β3 is used in human pluripotent stem cell maintenance medias, such as B8 media.2
High purity and bioactivity for reproducible results. Human TGF-β3 protein dimer, animal-origin free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

25501005001000
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buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

Summary

  • High purity TGF-β3 (Uniprot: P10600)

  • 12.7 kDa monomer, 25.4 kDa dimer

  • Expressed in E. coli

  • Animal-free (AOF) and carrier protein-free

  • Manufactured in Cambridge, UK

  • Lyophilized from acetonitrile, TFA

  • Resuspend in 10mM HCl (Reconstitution solution A) at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

Handling and storage FAQ

Featured applications

  • Human pluripotent stem cell maintenance

  • Mesoderm differentiation

Transforming growth factor beta-3, TGF-b3, TGFB3, prepro-transforming growth factor beta-3, arrhythmogenic right ventricular dysplasia 1 (ARVD, ARVD1)

Bioactivity

Zebrafish FGF2 / bFGF Qk002 protein bioactivity lot #011

TGF-β3 activity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. EC50 = 50 pg/ml.

Bioactivity is determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. Cells are treated (in triplicate) with a serial dilution of TGF-β3 for 6 hours. Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity. Data from Qk054 batch #104369.

Purity

Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

TGF-β3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R).  No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk054 batch #104369.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial:  >95%

Request CoA/animal-free certificate

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Comparison with other suppliers

Qkine TGF-β3 (Qk054) bioactivity was compared with an alternative supplier (Supplier 1) using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells (as above). Qkine TGF-β3 and Supplier 1 were found to have equivalent bioactivity, Qk054 EC50 = 50 pg/ml, Supplier 1 EC50 = 67 pg/ml (no significant difference).

Protein background

Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors.3

The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation.1 TGF-β3 has isoform-specific roles in embryonic palate fusion4 and wound healing.5

In culture, TGF-β3 is often used in human pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University.2 In the development of B8 media, Kuo et al.2 proved that while TGF-β1 can maintain iPSCs at 2ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1ng/ml, making it a cost-effective alternative to TGF-β1.

1. Morikawa, M., Derynck, R. & Miyazono, K. TGF-β and the TGF-β Family: Context-Dependent Roles in Cell and Tissue Physiology. Cold Spring Harb Perspect Biol 8, a021873 (2016).
2. Kuo, H.-H. et al. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports14, 256–270 (2020).
3. Huang, T., Schor, S. L. & Hinck, A. P. Biological Activity Differences between TGF-β1 and TGF-β3 Correlate with Differences in the Rigidity and Arrangement of Their Component Monomers. Biochemistry 53, 5737–5749 (2014).
4. Proetzel, G. et al. Transforming growth factor-β3 is required for secondary palate fusion. Nat Genet 11, 10.1038/ng1295-409 (1995).
5. Bandyopadhyay, B. et al. A “traffic control” role for TGFβ3: orchestrating dermal and epidermal cell motility during wound healing. J Cell Biol 172, 1093–1105 (2006).

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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