Hepatocyte differentiation

Expression of hepatocyte markers following culture with HGF NK1

Complete animal-free hepatocyte differentiation process

The complete set of fully animal-free growth factors for the differentiation of human pluripotent cells to hepatocytes is available. To ensure reproducibility of your endoderm differentiation protocols every lot is thoroughly tested for biochemical purity and bioactivity. Lot-specific quality control data and animal origin free (AOF/ADCF) certificates are available to facilitate planning for transition to pre-clinical studies and establishing future GMP-compliant processes.

Hepatocyte differentiation from iPSC protocol

Products for hepatocyte differentiation

Expansion – start here

Activin A (Qk001)
TGF-B1 PLUS (Qk010)
FGF-2 / bFGF 145 aa (Qk025)

Expansion – media optimization

Activin A PLUS (Qk005)
Follistatin-resistant Activin A (Qk035)
Thermostable bFGF / FGF2-G3 145 aa (Qk052)
Thermostable bFGF / FGF2-G3 154 aa (Qk053)
FGF-2 /bFGF 154 aa (Qk027)
FGF-2 / bFGF zebrafish (Qk002)

Differentiation

BMP4 (Qk038)
FGF-10 (Qk003)
OSM (Qk049)
HGF NK1 (Qk013)

Product focus: enhanced hepatocyte differentiation using HGF natural isoform NK1

The NK1 isoform is the smallest naturally occurring splice variant of HGF, it comprises the HGF N-terminal and first kringle domain. This isoform promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml, resulting in highly homogeneous expression of the hepatic marker, HNF4α. Qkine HGF NK1 is also completely animal-free (AOF), making it suitable for chemically defined media and reproducible scale-up.

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