UK +44 (0)1223 491486 USA toll free 1-866 877 2185
Register here for our next webinar
Human BDNF (brain-derived neurotrophic factor) protein is an important regulator of neural development, maintenance, and function, and is used in the differentiation of human pluripotent stem cell-derived neural progenitors to neurons and cortical organoid protocols.
Qkine human BDNF protein is high purity, animal-free and carrier protein-free for reproducible results.
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1mg may be despatched as 2 x 500µg
Buy online with secure credit card or purchase order or email email@example.com
Bulk and stock reservation available
High purity human BDNF protein (Uniprot: P23560)
>97%, by SDS-PAGE quantitative densitometry
Expressed in E. coli.
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10mM HCl, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Differentiation of iPSC derived neural progenitors to neurons
BDNF, brain-derived neurotrophic factor, abrineurin
Human, mouse, rat, bovine, porcine, canine, equine
Recombinant human FGF-2 protein
Recombinant human EGF protein
Recombinant human Noggin protein
Recombinant human/mouse/bovine/porcine TGFB1 PLUS protein
BDNF activity is determined using a rat C6 glioma cell proliferation assay. Cells were incubated with different concentrations of BDNF for 7 days before assay measurement. Cell proliferation is measured and normalized.
EC50 = 169.4 nM (2372 ng/mL).
Data from Qk050 lot #104346.
BDNF migrates as a single band at 14 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk050 batch #104346.
Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial >95%
We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
BDNF is a member of neurotrophin protein family, a family essential for the development, maintenance, and function of vertebrate nervous systems (Bekinschtein and von Bohlen und Halbach, 2020). BDNF regulates many aspects of neuronal development, including neuronal differentiation, growth, synapse formation, maturation and refinement, (Park and Poo, 2012), as well as neural stem cell survival, expansion, and differentiation (Chen et al. 2013). BDNF acts through the receptor TrkB to activate MAPK, PI3K and PLC-γ signalling cascades (Foltran and Diaz, 2016). BDNF is used in differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Brafman, 2015) and cortical organoids (Jacob et al. 2020).
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
Enter a URL (optional)
Choose a file
You must be logged in to join the discussion.
Show everythingShow all reviewsShow all questionsShow all photosShow helpful positive reviewsShow helpful negative reviewsShow unanswered questions
There are no contributions yet.
Username or email address *
Lost your password?
Email address *
Email address *
Already have an account? Log In
Don't have an account? Sign Up
Continue as a Guest
we respect your time and privacy
© 2023 Qkine Ltd. Company number 10470662 registered in England and Wales. All rights reserved