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Human TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. TGFβ1 is used extensively in E8-style induced pluripotent (iPSC) and embryonic (ESC) stem cell media.
High purity 24 kDa dimer comprising optimised mature domain of TGFβ1 protein, animal-free (AF) and carrier-protein free (CF). Our TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.
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1mg may be despatched as 2 x 500µg
high purity optimised human TGF-β1 protein (Uniprot: P01137)
24 kDa (dimer)
expressed in E. coli
animal-free (AOF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
lyophilized from acetonitrile, TFA
resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC
iPSC and ESC maintenance and expansion
chemically defined media
Recombinant human transforming growth factor-beta 1 (TGF-β1) protein is an essential growth factor in many iPSC and ESC maintenance media, including the commonly used chemically-defined E8-based stem cell culture medias. To date, recombinant human TGF-β1 has only been produced from mammalian cell protein expression systems (HEK or CHO), where endogenous protein contaminants, cost and animal-free status is a challenge. Our protein experts have optimized TGF-β1 allowing us to produce the first highly bioactive protein in an E. coli fully animal-free expression system.
As part of our ongoing mission to redefine industry standards for growth factor and cytokine biochemical quality, we’re delighted to introduce the first optimized, truly animal-free recombinant human TGF-β1 for our stem cell maintenance range.
TGF-β1 PLUS (Qk010) is highly bioactive compared to mammalian-expressed TGF-β1 preparations from other suppliers.
Comparative activity determined using a quantitative luciferase reporter assay in transiently transfected HEK293T cells. Cells were treated (in triplicate) with a serial dilution of TGF-β1. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. TGF-β1 PLUS EC50 1.4 pM. Suppliers 1 and 2 have EC50 ~3.5 pM and 38 pM respectively. Data from Qk010 lot #012 and #10273.
TGF-β1 PLUS (Qk010) dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form).
Purified recombinant protein (7 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk010 lot #012
further quality assays
mass spectrometry: single species with expected mass
analytical reversed-phase: single sharp peak
endotoxin: <0.005 EU/μg protein (below level of detection)
recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Immuno-staining for pluripotency markers Tra 1-60 and Nanog show high levels of expression in iPSC lines maintained in TGF-β1 PLUS (Qk010)-containing defined media. In this study, Qkine TGF-β1 PLUS performed better than the TGF-β1 used routinely by Stemnovate.
TGF-β1 PLUS (Qk010) maintains pluripotency and good colony morphology at 1 ng/ml in a chemically-defined serum and feeder-free iPSC culture. TGF-β1 PLUS used at 1 ng/ml; Qk025 FGF2 used at 100 ng/ml.
Stemnovate IPS media (chemically-defined, serum and feeder-free culture). Higher Nanog pluripotency marker immuno-staining in human iPSC cultured in Qkine FGF2 (Qk025) and TGF-β1 PLUS(Qk010).TGF-β1 PLUS used at 1 ng/ml.
All experiments have been conducted by the specialist stem cell biotechnology company, Stemnovate Limited, in Cambridge, UK.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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