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recombinant human TGF-β1 PLUS protein (Qk010)

Human TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. TGFβ1 is used extensively in E8-style induced pluripotent (iPSC) and embryonic (ESC) stem cell media.

High purity 24 kDa dimer comprising optimised mature domain of TGFβ1 protein, animal-free (AF) and carrier-protein free (CF).  Our  TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.

*Benchmark our TGF-β1 PLUS against your existing supplier*

Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.

25501005001000
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buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity optimised human TGF-β1 protein (Uniprot: P01137)

  • 24 kDa (dimer)

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • iPSC and ESC maintenance and expansion

  • chemically defined media

Transforming growth factor-beta 1, TGF-beta 1, TGFβ1, TGFB1, TGF B1

Recombinant human transforming growth factor-beta 1 (TGF-β1) protein is an essential growth factor in many iPSC and ESC maintenance media, including the commonly used chemically-defined E8-based stem cell culture medias. To date, recombinant human TGF-β1 has only been produced from mammalian cell protein expression systems (HEK or CHO), where endogenous protein contaminants, cost and animal-free status is a challenge.  Our protein experts have optimized TGF-β1 allowing us to produce the first highly bioactive protein in an E. coli fully animal-free expression system. 

As part of our ongoing mission to redefine industry standards for growth factor and cytokine biochemical quality, we’re delighted to introduce the first optimized, truly animal-free recombinant human TGF-β1 for our stem cell maintenance range.

Benefits:

  • Truly animal-free alternative for chemically-defined stem cell culture media
  • High potency in iPSC culture with high pluripotency (Nanog) marker expression
  • High purity, extensive biochemical data and exceptional batch-to-batch consistency

bioactivity

human-TGFb1-PLUS- Qk010 protein bioactivity lot #104273

TGF-β1 PLUS (Qk010) is highly bioactive compared to mammalian-expressed TGF-β1 preparations from other suppliers.

Comparative activity determined using a quantitative luciferase reporter assay in transiently transfected HEK293T cells. Cells were treated (in triplicate) with a serial dilution of TGF-β1. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. TGF-β1 PLUS EC50 1.4 pM. Suppliers 1 and 2 have EC50 ~3.5 pM and 38 pM respectively.  Data from Qk010 lot #012 and #10273.

purity

Human TGF beta 1 PLUS Qk010 protein purity SDS-PAGE lot #012

TGF-β1 PLUS (Qk010) dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form).

Purified recombinant protein (7 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk010 lot #012

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

organoid
iPSC/ESC maintenance
neural
hepatocyte
pancreatic
endodermal
mesodermal
epithelial
mesenchymal
hematopoietic

TGF-β1 PLUS maintains iPSC pluripotency with high Nanog expression

TGF-β1 PLUS is highly effective at maintaining iPSC pluripotency in chemically-defined, serum and feeder-free culture

Nanog Tra1-60 expression in iPSC maintained in TGFB1 PLUS

Immuno-staining for pluripotency markers Tra 1-60 and Nanog show high levels of expression in iPSC lines maintained in TGF-β1 PLUS (Qk010)-containing defined media. In this study, Qkine TGF-β1 PLUS performed better than the TGF-β1 used routinely by Stemnovate.

Phase contrast iPSC maintained in Qk010 TGF beta 1 and Qk025 FGF2 day 3

TGF-β1 PLUS (Qk010) maintains pluripotency and good colony morphology at 1 ng/ml in a chemically-defined serum and feeder-free iPSC culture. TGF-β1 PLUS used at 1 ng/ml; Qk025 FGF2 used at 100 ng/ml.

TGF-β1 PLUS in combination with Qkine FGF2 shows enhanced bioactivity when benchmarked against other suppliers

Comparison of Nanog immunofluorescence in cells maintained in TGF beta 1 from Qkine and supplier 1

Stemnovate IPS media (chemically-defined, serum and feeder-free culture). Higher Nanog pluripotency marker immuno-staining in human iPSC cultured in Qkine FGF2 (Qk025) and TGF-β1 PLUS(Qk010).TGF-β1 PLUS used at 1 ng/ml.

All experiments have been conducted by the specialist stem cell biotechnology company, Stemnovate Limited, in Cambridge, UK.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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