Recombinant human/bovine/porcine TGF-β1 PLUS protein (Qk010)
Human/bovine/porcine TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. TGF-β1 is used extensively in E8-style induced pluripotent (iPSC) and embryonic (ESC) stem cell media.
High purity 24 kDa dimer comprising optimised mature domain of TGFβ1 protein, animal-free (AF) and carrier-protein free (CF). Our TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.
*Benchmark our TGF-β1 PLUS against your existing supplier*
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

Question
Anonymous –
I have read your FAQ on protein handling. Still, resuspending proteins in low pH 10mM HCl is unusual for most proteins but apparently normal for TGF-Beta. Why is this the case for TGF-beta and would resuspending in pure water or PBS be acceptable for short-term storage? Thanks.
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Mairi Shepherd –
TGF-beta family proteins are very hydrophobic and consequently are poorly soluble and will adhere to plastic, we highly recommend following the handling guidance for both long- and short-term storage. We do not recommend resuspending in water or PBS as TGF-beta is insoluble in physiological buffer and the protein will precipitate. We advise storing the protein at a low pH to ensure solubility before dilution to cell culture media. A low pH will also aid the maintenance of the correct disulphide structure of the protein by minimising disulphide bond exchange reactions.
To save you the trouble of making a 10mM HCl solution, we ship vials of reconstitution solution A (10mM HCl) with proteins for which this is our recommended reconstitution buffer. Alternatively, you can make your own 1:1000 dilution of concentrated HCl. If you have any questions or would like to arrange a call with our protein team to discuss your concerns, please email support@qkine.com.
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