Recombinant human BMP-4 protein (Qk038)

Human BMP4 protein (bone morphogenetic protein 4) is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP4 protein plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.

Highly pure 24 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP4 protein (animal-free and carrier protein-free).

Our robust animal origin-free manufacturing platform, along with rigorous quality control procedures, ensures exceptional bioactivity and consistent performance from lot to lot, guaranteeing outstanding performance in your applications.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

500µg will be despatched as 5 x 100µg
1000µg will be despatched as 10x 100µg

Fast and free shipping.
Buy online with secure credit card or purchase order.

Summary

  • High purity optimised human BMP-4 protein (Uniprot: P12644)

  • >98%, by SDS-PAGE quantitative densitometry

  • 24 kDa (dimer)

  • Expressed in E. coli

  • Animal-free (AOF) and carrier protein-free.

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from acetonitrile, TFA

  • Resuspend in 10mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Featured applications

  • iPSC and ESC maintenance and expansion

  • Chemically defined media

Bone morphogenetic protein 4, Bone morphogenetic protein 2B, BMP-2B, BMP2B, DVR4

Human

Species similarity:
Bovine – 99%
Porcine – 99%
Mouse – 98%
Rat – 98%

Bioactivity

Human BMP4 Qk038 protein bioactivity lot #104294

BMP-4 activity is determined using the BMP4-responsive firefly luciferase reporter assay in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of BMP4 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.

EC50 = 30.2 pM (0.72 ng/mL).

Data from Qk038 lot #104294.

Purity

Human BMP4 Qk038 protein purity lot #104294

BMP-4  protein (Qk038) dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form).

Purified recombinant protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk038 lot #104294.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Analytical reversed-phase: single sharp peak

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Bone morphogenetic protein 4 (BMP4 protein) is part of the TGFβ superfamily of growth factors. BMP4 is a key regulator of embryogenesis with roles in gastrulation, establishment of a dorsal-ventral axis, and regulation of limb development (1).  As their name suggest the BMP family is involved in the development and maintenance of bone and cartilage; in adults BMP4 has roles bone and cartilage metabolism, as well as neurogenesis (2) and hematopoietic stem cell homing (3). BMP4 protein acts through two serine-threonine kinase receptors; Bone morphogenetic protein receptor (BMPR) 1 and BMPR2. Via these receptors BMP4 invokes Smad and map kinase pathways and consequently alters target gene transcription (4).

1. Bone Morphogenetic Protein (BMP) signaling in development and human diseases. Genes & Diseases 1, 87–105 (2014).
2. Xu, H. et al. The function of BMP4 during neurogenesis in the adult hippocampus in Alzheimer’s disease. Ageing Research Reviews 12, 157–164 (2013).
3. Khurana, S. et al. A novel role of BMP4 in adult hematopoietic stem and progenitor cell homing via Smad independent regulation of integrin-α4 expression. Blood 121, 781–790 (2013).
4. Bone Morphogenetic Protein (BMP) signaling in development and human diseases. Genes & Diseases 1, 87–105 (2014).

Additional resources

Qkine BMP4 technote

Technote: BMP-4 lot-to-lot consistency

Qkine BMP-4 bioactivity comparison technote

Technote: BMP-4 bioactivity

Key papers

Limited oxygen availability in standard cell culture alters metabolism and function in terminally-differentiated cells.
In The Embo Journal on 5 April 2024 by Tan, J., Virtue, S., et al.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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What others are saying

  1. Anonymous

    BMP4

    Anonymous

    A BMP-4 that is less than half the price of our previous supplier and has at least equal activity. All good as far as we are concerned.

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