Qk027 FGF2 / bFGF (human) Qkine protein vial

Human FGF2/bFGF Qk027 protein bioactivity lot #012

recombinant human FGF-2 protein (154 aa) (Qk027)

Recombinant human FGF2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein. Human bFGF / FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154aa form of FGF2 comprises the core structured region and N-terminal extension.

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enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

description
customer and collaborator data

summary

high purity human FGF-2 / bFGF protein comprising longer structured 154 aa region, residues 135-288 (Uniprot: P09038)
17 kDa
expressed in E. coli
animal-free (ADCF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
frozen in PBS
prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

iPSC / ESC expansion and differentiation

alternative protein names

Basic fibroblast growth factor, bFGF, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2

background references

1. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006).
2. Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006).
3. Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012).

bioactivity

FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 0.2 ng/ml (11.7 pM).

Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk027 lot #012

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

FGF2 migrates as major band at 17 kDa in non-reducing conditions and upon reduction.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk027 lot #010

further quality assays

mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
recovery from stock vial: >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

“We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for mouse Noggin, please contact us info@qkine.com”

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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