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recombinant human FGF-2 (bFGF) 154 aa protein (Qk027)
Human FGF-2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein. FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension.
High purity and bioactivity 17 kDa FGF-2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com
1mg may be despatched as 2 x 500µg
summary
high purity human FGF-2 / bFGF protein comprising longer structured 154 aa region, residues 135-288 (Uniprot: P09038)
17 kDa
expressed in E. coli
animal-free (ADCF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
frozen in PBS
prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC
featured applications
iPSC / ESC expansion and differentiation
1. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006).
2. Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006).
3. Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012).
species similarity:
bovine – 99%
porcine – 99%
rat – 97%
mouse – 94%
frequently used together
bioactivity
FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 0.2 ng/ml (11.7 pM).
Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk027 lot #012
purity
FGF2 migrates as major band at 17 kDa in non-reducing conditions and upon reduction.
Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk027 lot #010
further quality assays
mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
- recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
protein background
Fibroblast growth factor 2 (FGF2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF2 is used to support the maintenance of human embryonic stem cells1-3 and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.
“We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for mouse Noggin, please contact us info@qkine.com”
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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