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Home/recombinant growth factor & cytokine, FGF family, human, iPSC / ESC maintenance, organoid/recombinant human FGF-2 (bFGF) 154 aa protein (Qk027)

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recombinant human FGF-2 (bFGF) 154 aa protein (Qk027)

Human FGF-2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein.   FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension.

High purity and bioactivity 17 kDa FGF-2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

501005001000
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buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity human FGF-2 / bFGF protein comprising longer structured 154 aa region, residues 135-288 (Uniprot: P09038)

  • 17 kDa

  • expressed in E. coli

  • animal-free (ADCF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • frozen in PBS

  • prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • iPSC / ESC expansion and differentiation

bioactivity

Human FGF2/bFGF Qk027 protein bioactivity lot #012

FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 0.2 ng/ml (11.7 pM).

Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk027 lot #012

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

Human FGF2/bFGF Qk027 protein purity SDS-PAGE lot #010

FGF2 migrates as major band at 17 kDa in non-reducing conditions and upon reduction.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.  Data from Qk027 lot #010

further quality assays

  • mass spectrometry: single species with expected mass

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request CoA/animal-free certificate
download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

Fibroblast growth factor 2 (FGF2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF2 is used to support the maintenance of human embryonic stem cells1-3 and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

“We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for mouse Noggin, please contact us info@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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