Qk027 FGF2 / bFGF (human) Qkine protein vial

Human FGF2/bFGF Qk027 protein purity SDS-PAGE lot #010

Recombinant human FGF-2 (bFGF) 154 aa protein (Qk027)

Name: Recombinant human FGF-2 (bFGF) 154 aa protein (Qk027)
SKU: Qk027
Availability: InStock

Recombinant human FGF2 protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein. FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension.

High purity and bioactivity 17 kDa FGF-2 / bFGF protein, animal-free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
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Easy world-wide ordering, direct or through our distributors.

1mg will be despatched as 2 x 500µg

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Description

Summary

High purity human FGF-2 / bFGF protein comprising longer structured 154 aa region, residues 135-288 (Uniprot: P09038)
>98%, by SDS-PAGE quantitative densitometry
17 kDa
Expressed in E. coli
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from Tris/NaCl/CyS/mannitol
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired, store frozen at -20°C (short-term) or -80°C (long-term)

Handling and storage FAQ

Featured Applications

iPSC / ESC expansion and differentiation

Alternative protein names

Basic fibroblast growth factor, bFGF, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2

Background references

1. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006).
2. Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006).
3. Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012).

Species reactivity

human

species similarity:
bovine – 99%
porcine – 99%
rat – 97%
mouse – 94%

Frequently used together

Recombinant human activin A protein
Recombinant huamn EGF protein
Recombinant human TGFb1 protein

Alternative

Recombinant human FGF2 145aa protein
Recombinant zebrafish FGF2 protein
Recombinant mouse FGF2 protein
Recombinant bovine/procine FGF2 protein

This protein has recently been reformulated and will now arrive lyophilized. This change has been made to provide enhanced stability to ensure that we offer the best customer experience in terms of shipping and storage and to align with our company sustainability goals. Please visit our reconstitution page or contact customerservice@qkine.com for further information or to request the previous datasheet.

Bioactivity

FGF-2 154 aa activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.

EC50 = 11.3 pM (0.19 ng/mL).

Data from Qk027 lot #12.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

FGF2 migrates as major band at 17 kDa in non-reducing conditions and upon reduction.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk027 lot #010

Further quality assays

Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%

Request CoA/animal-free certificate

Download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein Background

Fibroblast growth factor 2 (FGF2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF2 is used to support the maintenance of human embryonic stem cells^1-3 and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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