UK +44 (0)1223 491486      USA toll free 1-866 877 2185

Home/iPSC /ESC differentiation, human, optimised by Qkine, organoid, R-spondins/recombinant human R-spondin 3 protein (Qk032)

Currency

GBP: Pound sterling (£)

recombinant human R-spondin 3 protein (Qk032)

Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.

17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.

Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.

25501005001000
Clear

buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity bioactive domain of human R-spondin 3 protein (Uniprot: Q9BXY4)

  • 17 kDa

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • chemically defined media for organoid culture

bioactivity

Human R-spondin 3 Qk032 protein bioactivity lot #010

Bioactivity using a Wnt reporter assay in HEK293T cells. HEK293T cells transfected with Wnt-responsive firefly luciferase reporter TOPflash are treated with increasing concentrations of R-spondin 3 in the presence of Wnt-conditioned media (1:8 dilution), in triplicate. The following day, luciferase activity is measured by luminescence. EC50 = 0.3 nM. Data from Qk032 #010

purity

Human R-spondin 3 Qk032 protein purity SDS-PAGE lot #010

R-spondin 3 bioactive domain migrates at 17 kDa in non-reducing (-βME) conditions and upon reduction (+βME).

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Data from Qk032 lot #010

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

organoid
iPSC/ESC maintenance
neural
hepatocyte
pancreatic
endodermal
mesodermal
epithelial
mesenchymal
hematopoietic

protein background

R-Spondin 3 (RSPO3) protein belongs to the R-spondin family comprizing four structurally-related, secreted ligands (RSPO 1-4)1. All contain furin-like and thrombospondin structural domains. R-spondins are activators of the canonical Wnt signaling pathway. Receptors include the leucine-rich repeat-containing G-protein coupled receptors (LGR) 4-62. In the colon, R-spondin 3 promotes stem cell recovery and epithelial repair via induction of Wnt.

An R-spondin, such as R-spondin 3, is an essential component of Wnt 3a, R-spondin-3 (or 1,2), Noggin (WRN) media used widely in propagation of intestinal organoids2. There is a drive towards improved reproducibility in stem cell and organoid culture. Conditioned media (spent media from cells that secrete growth factors such as Wnt and R-spondin, along with several other factors and waste products) is both challenging to manufacture consistently and conveniently, and a source of variability between labs3.

product documents

“We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for R-spondin 3, please contact us info@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

you may also find useful

Share your thoughts!

Let us know what you think...

What others are saying

There are no contributions yet.

×

Login

Register

Your personal data will be used to support your experience throughout this website, to manage access to your account, and for other purposes described in our privacy policy.

Continue as a Guest

Don't have an account? Sign Up

Go to Top