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Home/recombinant growth factor & cytokine, human, optimised by Qkine, organoid, R-spondins/recombinant human R-spondin 3 protein (Qk032)


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recombinant human R-spondin 3 protein (Qk032)

Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.

17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.

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1mg may be despatched as 2 x 500µg


  • high purity bioactive domain of human R-spondin 3 protein (Uniprot: Q9BXY4)

  • 17 kDa

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • chemically defined media for organoid culture

frequently used together


Human R-spondin 3 Qk032 protein bioactivity lot #010

Bioactivity using a Wnt reporter assay in HEK293T cells. HEK293T cells transfected with Wnt-responsive firefly luciferase reporter TOPflash are treated with increasing concentrations of R-spondin 3 in the presence of Wnt-conditioned media (1:8 dilution), in triplicate. The following day, luciferase activity is measured by luminescence. EC50 = 0.3 nM. Data from Qk032 #010


Human R-spondin 3 Qk032 protein purity SDS-PAGE lot #010

R-spondin 3 bioactive domain migrates at 17 kDa in non-reducing (-βME) conditions and upon reduction (+βME).

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Data from Qk032 lot #010

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request CoA/animal-free certificate
download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

R-Spondin 3 (RSPO3) protein belongs to the R-spondin family comprizing four structurally-related, secreted ligands (RSPO 1-4)1. All contain furin-like and thrombospondin structural domains. R-spondins are activators of the canonical Wnt signaling pathway. Receptors include the leucine-rich repeat-containing G-protein coupled receptors (LGR) 4-62. In the colon, R-spondin 3 promotes stem cell recovery and epithelial repair via induction of Wnt.

An R-spondin, such as R-spondin 3, is an essential component of Wnt 3a, R-spondin-3 (or 1,2), Noggin (WRN) media used widely in propagation of intestinal organoids2. There is a drive towards improved reproducibility in stem cell and organoid culture. Conditioned media (spent media from cells that secrete growth factors such as Wnt and R-spondin, along with several other factors and waste products) is both challenging to manufacture consistently and conveniently, and a source of variability between labs3.

“We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for R-spondin 3, please contact us

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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