|recombinant human BMP-4 protein (Qk038)||£200.00 – £3,000.00|
Human BMP4 (bone morphogenetic protein 4) protein is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP4 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.
Highly pure 26 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP4 protein (animal-free and carrier protein-free).
|Show details||growth-factor-cytokine bmp-family epithelial hepatocyte human ipsc-esc-maintenance mesenchymal neural organoid|
|recombinant human EGF protein (Qk011)||£50.00 – £175.00|
Human EGF protein is a potent EGF-family growth factor used in many human and mouse organoid and stem cell culture systems including intestinal and tumor (cancer) organoid culture.
A pure 6.3 kDa biologically active mature human epidermal growth factor. Our recombinant EGF protein is manufactured to the highest purity in E. coli and extensively tested, making it ideal for use in chemically defined iPSC, ESC and organoid culture media.
|Show details||growth-factor-cytokine egf-family epithelial human ipsc-esc-maintenance neural organoid|
|recombinant human FGF-2 (bFGF) 145 aa protein (Qk025)||£100.00 – £600.00|
Human FGF2 /bFGF protein (145 aa) is our gold-standard and recommended FGF2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation.
High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF) comprising the core structured region (145 amino acids) sufficient for full biological activity.
|Show details||growth-factor-cytokine fgf-family human ipsc-esc-maintenance organoid|
|recombinant human FGF-2 (bFGF) 154 aa protein (Qk027)||£100.00 – £600.00|
Human FGF-2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein. FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension.
High purity and bioactivity 17 kDa FGF-2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).
|Show details||growth-factor-cytokine fgf-family human ipsc-esc-maintenance organoid|
|recombinant FGF2-G3 145 aa protein (Qk052)||£120.00 – £3,000.00|
FGF2-G3 (145 aa) protein is a thermostable engineered form of FGF-2 (bFGF). Qk052 comprises the 145 aa form of FGF-2 (Qk025) with the nine amino acid substitutions developed by Dvorak et al. 2018. This increases the functional half-life of the protein from <10 h (wild-type) to >7 days (FGF2-G3 145 aa).
FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. This new 145 aa thermostable FGF-2 protein allows direct comparison of efficacy in systems using FGF-2 145 aa (Qk025) for chemically defined stem cell and organoid culture media, and cultured meat media development.
High purity 16 kDa bioactive FGF2-G3 145 aa protein. Animal-free (AOF), carrier protein-free and with no His-tag.
|Show details||growth-factor-cytokine cellular-agriculture fgf-family human mouse neural optimised-by-qkine organoid porcine rat|
|recombinant bovine/porcine FGF-2 (bFGF) protein (Qk040)||£100.00 – £600.00|
Bovine/porcine FGF2 protein (bFGF/basic FGF) for development of species specific bovine (cow) and porcine (pig) cellular agriculture protocols and veterinary research applications.
FGF-2 is used extensively in maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC)1-3 and for enhancement of proliferation in primary cell culture.
High purity 16 kDa FGF-2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).
|Show details||growth-factor-cytokine bovine cellular-agriculture fgf-family ipsc-esc-maintenance organoid porcine|
|recombinant mouse FGF-2 (bFGF) protein (Qk042)||£100.00 – £600.00|
mouse FGF2 / bFGF protein is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.1,2
High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).
|Show details||growth-factor-cytokine fgf-family ipsc-esc-maintenance mouse mouse-es-cell organoid|
|recombinant zebrafish FGF-2 (bFGF) protein (Qk002)||£100.00 – £400.00|
Zebrafish FGF-2 protein (bFGF/basic FGF) has been used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC); used in the original Ludwig et al feeder-free culture of embryonic stem cells protocols 1-3.
High purity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF). This version of recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge.
|Show details||growth-factor-cytokine fgf-family hepatocyte ipsc-esc-maintenance organoid|
|recombinant human KGF (FGF-7) protein (Qk046)||£200.00 – £3,000.00|
human KGF / FGF-7 protein is a member of the FGF family secreted by mesenchymal cells and has a paracrine effect on neighbouring epithelial cells.1 KGF is used in the culture of lung, breast, liver, and bladder organoids.2 In vivo, KGF is involved in wound healing and development of kidney, thymus, and hippocampus.1
High purity and bioactivity 19 kDa human FGF-7 / KGF protein, animal-origin free (AOF) and carrier-protein free (CF).
|Show details||epithelial fgf-family human organoid|
|recombinant human FGF-9 protein (Qk039)||£120.00 – £1,600.00|
Human FGF-9 protein is a member of the fibroblast growth factor (FGF) family, FGF-9 is involved in many developmental processes including neural and skeletal development. Recombinant FGF9 is used frequently in the generation of kidney organoids.
Highly pure and bioactive 23 kDa FGF-9 protein, animal-derived component free (ACDF) and carrier-protein free (CF)
|Show details||fgf-family human mesenchymal neural organoid|
|recombinant human/rat/bovine/porcine FGF-10 protein (Qk003)||£160.00 – £1,200.00|
Human/rat/porcine/bovine FGF10 protein promotes lung organoid formation and induces branching morphology. FGF10 protein is used widely in organoid culture, embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) differentiation, and for the study of epithelial to mesenchymal transition and tumor metastasis.
High purity and bioactivity 17 kDa non-glycosylated, bioactive domain of human fibroblast growth factor 10, animal-derived component free (ACDF) and carrier-protein free (CF).
|Show details||growth-factor-cytokine bovine fgf-family hepatocyte human organoid porcine rat|
|recombinant human/bovine/porcine Gremlin 1 protein (Qk015)||£100.00 – £1,200.00|
Human/bovine/porcine Gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to Noggin for optimisation of intestinal organoid culture and iPSC differentiation.
Qk015 has been optimized by our experts for exceptionally high-purity production in E.coli and bioactivity. 18 kDa high purity dimeric protein, animal-free and carrier protein free
|Show details||growth-factor-cytokine bmp-family bovine human ipsc-esc-maintenance optimised-by-qkine organoid porcine|
|recombinant human HGF (NK1) protein (Qk013)||£160.00 – £1,200.00|
Human HGF (NK1) protein is a potent, high purity NK1 isoform of human hepatocyte growth factor (HGF) that promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α. The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.
20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
|Show details||growth-factor-cytokine hepatocyte human optimised-by-qkine organoid plasminogen-gf-family-hgf|
|recombinant human noggin protein (Qk034)||£160.00 – £2,400.00|
Human noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.
Qkine human noggin protein is a highly pure 46kDa bioactive dimer for reproducible results in organoid culture.
|Show details||growth-factor-cytokine bmp-family bmp-inhibitors-noggin human ipsc-esc-maintenance mouse-es-cell neural organoid|
|recombinant mouse/rat noggin protein (Qk033)||£160.00 – £2,400.00|
Mouse/rat noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.
Qkine recombinant mouse noggin protein is a highly pure 46kDa bioactive dimer for reproducible results (animal-free and carrier-protein free).
|Show details||growth-factor-cytokine bmp-family bmp-inhibitors-noggin epithelial ipsc-esc-maintenance mouse mouse-es-cell neural organoid rat|
|recombinant human BDNF protein (Qk050)||£160.00 – £2,400.00|
Human BDNF (brain-derived neurotrophic factor) protein is an important regulator of neural development, maintenance, and function, and is used in the differentiation of human pluripotent stem cell-derived neural progenitors to neurons and cortical organoid protocols.
Qkine human BDNF protein is high purity, animal-free and carrier protein-free for reproducible results.
|Show details||human neural neurotrophin organoid|
|recombinant human R-spondin 1 protein (Qk006)||£120.00 – £1,600.00|
Human R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signalling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.
13kDa highly pure, bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Qk006 is animal-free and not glycosylated to ensure production of homogeneous protein and enhanced comparability between batches. Used to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
|Show details||growth-factor-cytokine human ipsc-esc-maintenance optimised-by-qkine organoid r-spondins|
|recombinant human R-spondin 1 LR5 protein (Qk031)||£120.00 – £180.00|
Recombinant R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor. In epithelial tissues LGR5 marks the stem cell population. This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population. R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells. This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).
|Show details||growth-factor-cytokine ipsc-esc-maintenance optimised-by-qkine organoid pioneering-proteins r-spondins|
|recombinant human R-spondin 3 protein (Qk032)||£120.00 – £1,600.00|
Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.
17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.
|Show details||growth-factor-cytokine human optimised-by-qkine organoid r-spondins|