Human BMP4 (bone morphogenetic protein 4) protein is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP4 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.

Highly pure 26 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP4 protein (animal-free and carrier protein-free).

Human FGF-9 protein is a member of the fibroblast growth factor (FGF) family, FGF-9 is involved in many developmental processes including neural and skeletal development. Recombinant FGF9 is used frequently in the generation of kidney organoids.

Highly pure and bioactive 23 kDa FGF-9 protein, animal-derived component free (ACDF) and carrier-protein free (CF)

Human FGF2 /bFGF protein (145 aa) is our gold-standard and recommended FGF2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation.

High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF) comprising the core structured region (145 amino acids) sufficient for full biological activity.

Human FGF2 / bFGF protein (154 aa) is a highly bioactive, long–form of human fibroblast growth factor 2 protein.   Human bFGF / FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF2 comprises the core structured region and N-terminal extension.

High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).

mouse FGF2 / bFGF protein is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.^1,2

High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).

Human HGF (NK1) protein is a potent, high purity NK1 isoform of human hepatocyte growth factor (HGF) that promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α. The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.

20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.

Human noggin supports growth of organoids derived from epithelial tissues and iPSC and ESC differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; epithelial organoid culture (WNR media), maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.

Qkine human noggin protein is a highly pure 46kDa bioactive dimer for reproducible results in organoid culture.

Mouse/rat noggin supports growth of organoids derived from mouse epithelial tissues and stem cell differentiation. Noggin protein is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids; maintenance of embryonic stem cells and differentiation into neural lineages.

Qkine recombinant mouse noggin protein is a highly pure 46kDa bioactive dimer for reproducible results (animal-free and carrier-protein free).

Human R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signalling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.

13kDa highly pure, bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture.  Qk006 is animal-free and not glycosylated to ensure production of homogeneous protein and enhanced comparability between batches. Used to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.

Recombinant R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor.  In epithelial tissues LGR5 marks the stem cell population.  This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population.  R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells.  This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).

Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.

17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.