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Human FGF2 /bFGF protein (145 aa) is our gold-standard and recommended FGF2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation.
High purity and bioactivity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF) comprising the core structured region (145 amino acids) sufficient for full biological activity.
Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.
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1mg may be despatched as 2 x 500µg
high purity human FGF2 / basic FGF, 145aa form comprising residues 144-288 (Uniprot: P09038)
expressed in E. coli
animal-free (AOF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
frozen in PBS at 1 mg/ml
prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC
expansion of induced pluripotent, embryonic and mesenchymal stem cells
1. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006).
2. Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006).
3. Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012).
FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells.
Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 103 pg/ml (6.3 pM). Data are from Qk025 batch #014
FGF2 migrates as major band at 17 kDa in non-reducing (-βME) conditions and upon reduction (+βME). The higher molecular mass band at 35 kDa is a dimer that we always see in our highly purified human FGF2, the presence of this does not affect biological activity.
Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk025 lot #014
further quality assays
mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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Keele University, UK
– October 30, 2018
“Just to let you know, I’ve tested the GFs and the Activin A and human FGF worked exceptionally well compared to what I usually use”. Keele, UK.
A 14-day directed differentiation protocol was applied to human embryonic stem cells in order to generate chondroprogenitors. The use of Qkine human Activin-A and human FGF resulted in significantly higher levels of ACAN, COL2 and SOX9 gene expression and significantly lower levels of OCT4 gene expression.
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