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Recombinant human FGF-2 (bFGF) 145 aa protein (Qk025)

Human FGF-2 / bFGF protein (145 aa) is our gold-standard and recommended FGF-2 / bFGF protein for induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) maintenance, and iPSC and mesenchymal stem cell (MSC) proliferation and differentiation.

Highly pure and bioactive 17 kDa FGF-2 / bFGF protein animal origin-free (AOF) and carrier protein-free (CF) comprising the core structured region (145 aa) sufficient for full biological activity.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1000µg will be despatched as 2 x 500µg

Fast and free shipping.
Buy online with secure credit card or purchase order.
For any questions, please email orders@qkine.com

Summary

  • High purity human FGF2 / basic FGF, 145aa form comprising residues 144-288 (Uniprot: P09038)

  • >98%, by SDS-PAGE quantitative densitometry

  • 17 kDa

  • Expressed in E. coli

  • Animal-free (AOF) and carrier protein-free.

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from Tris/NaCl/CyS/mannitol

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired, store frozen at -20°C (short-term) or -80°C (long-term)

Featured applications

  • Expansion of induced pluripotent, embryonic and mesenchymal stem cells

  • Cell expansion

Basic fibroblast growth factor, bFGF, FGF-β, FGF2, Fibroblast growth factor-basic, HBGF-2

Human

Species similarity:
bovine – 99%
porcine – 99%
rat – 97%
mouse – 94%

This protein has recently been reformulated and will now arrive lyophilized. This change has been made to provide enhanced stability to ensure that we offer the best customer experience in terms of shipping and storage and to align with our company sustainability goals. Please visit our reconstitution page or contact customerservice@qkine.com for further information or to request the previous datasheet.

Bioactivity

Human FGF2 / bFGF Qk025 protein bioactivity lot #014

FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells.

Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 103 pg/ml (6.3 pM).  Data are from Qk025 batch #014

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

Human FGF2 / bFGF Qk025 protein purity SDS-PAGE lot #014

FGF2 migrates as major band at 17 kDa in non-reducing (-βME) conditions and upon reduction (+βME). The higher molecular mass band at 35 kDa is a dimer that we always see in our highly purified human FGF2, the presence of this does not affect biological activity.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.  Data from Qk025 lot #014

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Fibroblast growth factors (FGFs) are a family of growth factors which regulate a wide range of essential biological functions including cell proliferation and survival, migration and differentiation [1]. The human FGF family has 18-22 members grouped into 6-7 subfamilies based on sequence homology and phylogeny [2]. The FGF family of growth factors have critical roles during both vertebrate and invertebrate embryonic development, in adult cells they modulate tissue maintenance, wound healing and angiogenesis [1,2].

The FGF ligands bind to 4 receptors (FGFR1-4), transmembrane receptors with intracellular tyrosine kinase activity [2, 3]. Once activated FGFRs recruit Src homology-2 (SH2) or phosphotyrosine binding (PTB) domain-containing signaling proteins leading to activation of intracellular signaling pathways [1]. The main signaling pathways activated by FGF binding are the RAS/MAP kinase pathway, PI3 kinase/Akt pathway, and PLCγ pathways [1-3].

Fibroblast growth factor 2 (FGF-2) also known as basic fibroblast growth factor (bFGF) has a broad range of physiological roles including regulation of cell growth, survival and proliferation. FGF-2 is one of the FGFs which binds to and signals through all four of the FGFRs [4].

FGF-2 is an essential growth factor for the maintenance of pluripotency in human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) [4] and mesenchymal stem cells (MSCs) [5]. FGF2 preserves pluripotency through interaction with PI3 kinase/Akt, ERK1/2, JAK/STAT and PLCγ pathways by activation of Activin A. In feeder culture systems FGF-2 stimulates production of essential growth factors and cytokines from the feeder layer [5].

FGF-2 and TGFβ1 are required for maintenance of iPSC in feeder free culture, Qkine produces high quality, bioactive and animal origin-free FGF-2 (Qk025) and TGFβ1 (Qk010) for stem cell culture. However, FGF-2 has poor thermostability in culture so, to prevent the need for daily media changes, Qkine FGF2-G3 has been engineered to be highly thermostable allowing weekend-free culture of iPSCs.

[1] Y. R. Yun, J. E. Won, E. Jeon, S. Lee, W. Kang, H. Jo, J. H. Jang, U. S. Shin, H. W. Kim, ‘Fibroblast growth factors: biology, function, and application for tissue regeneration’, J. Tissue Eng. vol. 7, pp. 2010:218142, Nov. 2010, doi: 10.4061/2010/218142.

[2] Y. Xie, N. Su, J. Yang et al, ‘FGF/FGFR signaling in health and disease’, Sig. Transduct. Target. Ther. vol. 5, no. 181, 2020, doi.org/10.1038/s41392-020-00222-7.

[3] A. Beenken, M. Mohammadi, ‘The FGF family: biology, pathophysiology and therapy’, Nat. Rev. Drug Discov. vol. 8, no. 3, pp. 235-53. Mar. 2009, doi: 10.1038/nrd2792.

[4] M. Mossahebi-Mohammadi, M. Quan, J. S. Zhang and X. Li, ‘FGF Signaling Pathway: A Key Regulator of Stem Cell Pluripotency’, Front. Cell Dev. Biol. vol. 8, pp. 79, 2020, doi: 10.3389/fcell.2020.00079.

[5] W.T. Lai, V. Krishnappa, D.G. Phinney, ‘Fibroblast growth factor 2 (Fgf2) inhibits differentiation of mesenchymal stem cells by inducing Twist2 and Spry4, blocking extracellular regulated kinase activation, and altering Fgf receptor expression levels’, Stem Cells, vol. 29, no. 7, pp. 1102-11, Jul. 2011, doi: 10.1002/stem.661.

What is FGF-2?

Fibroblast growth factor 2 (FGF-2), also known as basic fibroblast growth factor (bFGF) is a growth factor and signaling protein.

Where is FGF-2 found?

FGF2 is expressed in a developmental and tissue specific manner. It’s expression is tightly controlled in normal tissues and it can be detected in all major tissues.

What does FGF-2 do?

FGF2 is essential for normal embryonic development. It has roles in cell survival and proliferation, angiogenesis, tumorigenesis, wound healing and tissue repair.

What does FGF-2 bind to?

FGF2 binds to and signals though all four of the FGF receptors FGFR1-4.

What is the function of the FGF receptor?

FGFRs phosphorylate specific tyrosine residues and activate the RAS-MAPK, PI3K-AKT, PLCγ, and STAT intracellular signaling pathways.

How is FGF-2 used in stem cell culture?

FGF2 is used to maintain the pluripotency of stem cells in culture.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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What others are saying

  1. Keele University, UK

    Just to let you know, I've tested the GFs and the Activin A and human FGF worked exceptionally well compared to what I usually use

    Keele University, UK

    “Just to let you know, I’ve tested the GFs and the Activin A and human FGF worked exceptionally well compared to what I usually use”. Keele, UK.

    A 14-day directed differentiation protocol was applied to human embryonic stem cells in order to generate chondroprogenitors. The use of Qkine human Activin-A and human FGF resulted in significantly higher levels of ACAN, COL2 and SOX9 gene expression and significantly lower levels of OCT4 gene expression.

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