recombinant mouse FGF-2 (bFGF) protein (Qk042)

mouse FGF2 / bFGF protein is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.^1,2

High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.

buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

description
customer & collaborator data

summary

high purity murine FGF-2/bFGF (Uniprot: P15655)
16 kDa
expressed in E. coli
animal-free (AOF) and carrier protein-free
manufactured in our Cambridge, UK laboratories
frozen in PBS
prepare single use aliquots, add carrier protein if desired, store frozen at -20 ^oC or -80 ^oC

handling and storage FAQ

featured applications

stem cell expansion and maintenance; primary cell culture

alternative protein names

Basic fibroblast growth factor, bFGF, FGFβ, FGF2, Fibroblast growth factor-basic, HBGF2

background references

Mossahebi-Mohammadi, Majid, et al. ‘FGF Signaling Pathway: A Key Regulator of Stem Cell Pluripotency’. Frontiers in Cell and Developmental Biology, vol. 8, 2020. Frontiers, doi:10.3389/fcell.2020.00079.
Greber, Boris, et al. ‘Conserved and Divergent Roles of FGF Signaling in Mouse Epiblast Stem Cells and Human Embryonic Stem Cells’. Cell Stem Cell, vol. 6, no. 3, Mar. 2010, pp. 215–26. www.cell.com, doi:10.1016/j.stem.2010.01.003.

species reactivity

mouse

bioactivity

Mouse FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 93 pg/ml (5.7 pM).

Cells are treated in triplicate with a serial dilution of mouse FGF-2 (Qk042) for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk042 lot #104309.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

Mouse FGF-2 migrates as predominantly as a single band at 16 kDa in non-reducing (NR) conditions, although a small amount of dimer can be seen. Upon reduction (R), only the 16 kDa band is visible. No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk042 lot #104309.

further quality assays

mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
recovery from stock vial: >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

protein background

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for mouse (murine) FGF2 / bFGF, please contact us info@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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