Recombinant mouse FGF-2 (bFGF) protein (Qk042)

Name: Recombinant mouse FGF-2 (bFGF) protein (Qk042)
SKU: Qk042
Availability: InStock

Recombinant mouse FGF2 protein/ bFGF is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.^1,2

High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg may be despatched as 2 x 500µg

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Description

Summary

High purity murine recombinant mouse FGF2 protein/bFGF (Uniprot: P15655)
>97%, by SDS-PAGE quantitative densitometry
16 kDa
Expressed in E. coli
Animal-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Frozen in PBS
Prepare single use aliquots, add carrier protein if desired, store frozen at -20 ^oC or -80 ^oC

handling and storage FAQ

Featured applications

Stem cell expansion and maintenance; primary cell culture

Alternative protein names

Basic fibroblast growth factor, bFGF, FGFβ, FGF2, Fibroblast growth factor-basic, HBGF2

Background references

Mossahebi-Mohammadi, Majid, et al. ‘FGF Signaling Pathway: A Key Regulator of Stem Cell Pluripotency’. Frontiers in Cell and Developmental Biology, vol. 8, 2020. Frontiers, doi:10.3389/fcell.2020.00079.
Greber, Boris, et al. ‘Conserved and Divergent Roles of FGF Signaling in Mouse Epiblast Stem Cells and Human Embryonic Stem Cells’. Cell Stem Cell, vol. 6, no. 3, Mar. 2010, pp. 215–26. www.cell.com, doi:10.1016/j.stem.2010.01.003.

Species reactivity

mouse

Frequently used together

Recombinant human activin A protein
Recombinant human TGFb1 PLUS protein

Alternative

Recombinant human FGF2 145aa protein
Recombinant human FGF2 154aa protein
Recombinant zebrafish FGF2 protein
Recombinant bovine/procine FGF2 protein

Bioactivity

Mouse FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 93 pg/ml (5.7 pM).

Cells are treated in triplicate with a serial dilution of mouse FGF-2 (Qk042) for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk042 lot #104309.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

Recombinant mouse FGF2 protein migrates as predominantly as a single band at 16 kDa in non-reducing (NR) conditions, although a small amount of dimer can be seen. Upon reduction (R), only the 16 kDa band is visible. No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk042 lot #104309.

Further quality assays

Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%

Request CoA/animal-free certificate

Download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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