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Home/recombinant growth factor & cytokine, FGF family, iPSC / ESC maintenance, mouse, mouse ES cell, organoid/recombinant mouse FGF-2 (bFGF) protein (Qk042)

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recombinant mouse FGF-2 (bFGF) protein (Qk042)

mouse FGF2 / bFGF protein is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.1,2

High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg may be despatched as 2 x 500µg

Buy online with secure credit card or purchase order or email orders@qkine.com
Bulk and stock reservation available

summary

  • high purity murine FGF-2/bFGF (Uniprot: P15655)

  • 16 kDa

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free

  • manufactured in our Cambridge, UK laboratories

  • frozen in PBS

  • prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC

featured applications

  • stem cell expansion and maintenance; primary cell culture

Basic fibroblast growth factor, bFGF, FGFβ, FGF2, Fibroblast growth factor-basic, HBGF2

  1. Mossahebi-Mohammadi, Majid, et al. ‘FGF Signaling Pathway: A Key Regulator of Stem Cell Pluripotency’. Frontiers in Cell and Developmental Biology, vol. 8, 2020. Frontiers, doi:10.3389/fcell.2020.00079. 
  2. Greber, Boris, et al. ‘Conserved and Divergent Roles of FGF Signaling in Mouse Epiblast Stem Cells and Human Embryonic Stem Cells’. Cell Stem Cell, vol. 6, no. 3, Mar. 2010, pp. 215–26. www.cell.com, doi:10.1016/j.stem.2010.01.003.

mouse

frequently used together

Recombinant human activin A protein
Recombinant human TGFb1 PLUS protein

alternative

Recombinant human FGF2 145aa protein
Recombinant human FGF2 154aa protein
Recombinant zebrafish FGF2 protein
Recombinant bovine/procine FGF2 protein

bioactivity

Zebrafish FGF2 / bFGF Qk002 protein bioactivity lot #011

Mouse FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 93 pg/ml (5.7 pM).

Cells are treated in triplicate with a serial dilution of mouse FGF-2 (Qk042) for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk042 lot #104309.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

Mouse FGF-2 migrates as predominantly as a single band at 16 kDa in non-reducing (NR) conditions, although a small amount of dimer can be seen. Upon reduction (R), only the 16 kDa band is visible.  No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk042 lot #104309.

further quality assays

  • mass spectrometry: single species with expected mass

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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Reconstitution selector

Recommended buffer:

10 mM HCl
from June 2021 we will send this buffer to you with your product to make reconstitution easy
H20
this protein is provided frozen, dilution can be performed with your choice of media or buffer

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