Recombinant mouse FGF-2 (bFGF) protein (Qk042)

Recombinant mouse FGF2 protein/ bFGF is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.1,2

High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1000µg will be despatched as 2 x 500µg

Buy online with secure credit card or purchase order or email
Bulk and stock reservation available


  • High purity murine recombinant mouse FGF2 protein/bFGF (Uniprot: P15655)

  • >98%, by SDS-PAGE quantitative densitometry

  • 16 kDa

  • Expressed in E. coli

  • Animal-free (AOF) and carrier protein-free

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from Tris/NaCl/CyS/mannitol

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC (short-term) or -80 oC (long-term)

Featured applications

  • Stem cell expansion and maintenance; primary cell culture

Basic fibroblast growth factor, bFGF, FGFβ, FGF2, Fibroblast growth factor-basic, HBGF2
  1. Mossahebi-Mohammadi, Majid, et al. ‘FGF Signaling Pathway: A Key Regulator of Stem Cell Pluripotency’. Frontiers in Cell and Developmental Biology, vol. 8, 2020. Frontiers, doi:10.3389/fcell.2020.00079. 
  2. Greber, Boris, et al. ‘Conserved and Divergent Roles of FGF Signaling in Mouse Epiblast Stem Cells and Human Embryonic Stem Cells’. Cell Stem Cell, vol. 6, no. 3, Mar. 2010, pp. 215–26., doi:10.1016/j.stem.2010.01.003.

This protein has recently been reformulated and will now arrive lyophilized. This change has been made to provide enhanced stability to ensure that we offer the best customer experience in terms of shipping and storage and to align with our company sustainability goals. Please visit our reconstitution page or contact for further information or to request the previous datasheet.


Zebrafish FGF2 / bFGF Qk002 protein bioactivity lot #011

Mouse FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 93 pg/ml (5.7 pM).

Cells are treated in triplicate with a serial dilution of mouse FGF-2 (Qk042) for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk042 lot #104309.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340


Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

Recombinant mouse FGF2 protein migrates as predominantly as a single band at 16 kDa in non-reducing (NR) conditions, although a small amount of dimer can be seen. Upon reduction (R), only the 16 kDa band is visible.  No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk042 lot #104309.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

Share your thoughts!

Let us know what you think...

What others are saying

There are no contributions yet.




Your personal data will be used to support your experience throughout this website, to manage access to your account, and for other purposes described in our privacy policy.

Continue as a Guest

Don't have an account? Sign Up

Amazon gift voucher available for growth factor and cytokine reviews

Receive an Amazon gift voucher when you leave us a review.

£25, $30 or €30 for reviews with an image and £10, $15 or €15 for reviews without an image
Terms and conditions apply