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mouse FGF2 / bFGF protein is an important factor in the maintenance of mouse epiblast-derived stem cells (EpiSC). Mouse naïve pluripotency is maintained by LIF, BMP4, and Wnt signalling pathways. However, the primed state of pluripotency in mouse EpiSCs is more similar to hESC and hiPSC, being maintained by FGF2 and TGFβ/Activin/Nodal pathways.1,2
High purity and bioactivity 16 kDa murine bFGF / FGF2 (basic FGF) protein, animal-origin free (AOF) and carrier-protein free (CF).
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1mg may be despatched as 2 x 500µg
Buy online with secure credit card or purchase order or email email@example.com
Bulk and stock reservation available
high purity murine FGF-2/bFGF (Uniprot: P15655)
expressed in E. coli
animal-free (AOF) and carrier protein-free
manufactured in our Cambridge, UK laboratories
frozen in PBS
prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC
stem cell expansion and maintenance; primary cell culture
Recombinant human activin A protein
Recombinant human TGFb1 PLUS protein
Recombinant human FGF2 145aa protein
Recombinant human FGF2 154aa protein
Recombinant zebrafish FGF2 protein
Recombinant bovine/procine FGF2 protein
Mouse FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 93 pg/ml (5.7 pM).
Cells are treated in triplicate with a serial dilution of mouse FGF-2 (Qk042) for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk042 lot #104309.
*Promega pGL4.33[luc2P/SRE/Hygro] #E1340
Mouse FGF-2 migrates as predominantly as a single band at 16 kDa in non-reducing (NR) conditions, although a small amount of dimer can be seen. Upon reduction (R), only the 16 kDa band is visible. No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk042 lot #104309.
mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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