Qk002 FGF-2 / bFGF (zebrafish) Qkine protein vial

Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

recombinant zebrafish FGF-2 (bFGF) protein (Qk002)

Zebrafish FGF-2 protein (bFGF/basic FGF) has been used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC); used in the original Ludwig et al feeder-free culture of embryonic stem cells protocols ^1-3.

High purity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF). This version of recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge.

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1mg may be despatched as 2 x 500µg

description
customer & collaborator data

summary

high purity zebrafish Danio rerio FGF2/bFGF (residues 2-154, Uniprot: B3DGE3) as described in Ludwig et al.².
17 kDa
expressed in E. coli
animal-free (AOF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
frozen in PBS
prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

stem cell expansion and maintenance; recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge

alternative protein names

Basic fibroblast growth factor, bFGF, FGFβ, FGF2, Fibroblast growth factor-basic, HBGF2

background references

1. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006).
2. Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006).
3. Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012).

bioactivity

zebrafish FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells.

Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 0.42 ng/ml (24.7 pM). Data are from Qk002 batch #011

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

FGF2 migrates as major band at 17 kDa in non-reducing (-βME) conditions and upon reduction (+βME). The higher molecular mass band at 35 kDa is a dimer that we always see in our highly purified zebrafish FGF2 protein, the presence of this does not affect biological activity. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Data from Qk002 lot #011

further quality assays

mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
recovery from stock vial: >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

protein background

Fibroblast growth factor 2 (FGF2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for zFGF2, please contact us info@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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What others are saying

FGF2 for pancreatic cancer sphere systems

Stem cell researcher, University of Oxford, UK – July 20, 2018

We have also used the FGF2 for pancreatic cancer sphere systems and it seems to support the growth of the cells as we would expect. University of Oxford, UK.

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