Home/recombinant growth factor & cytokine, FGF family, hepatocyte, iPSC / ESC maintenance, organoid/Recombinant zebrafish FGF-2 (bFGF) protein (Qk002)


GBP: £ ^

Recombinant zebrafish FGF-2 (bFGF) protein (Qk002)

Zebrafish FGF-2 protein (bFGF/basic FGF) has been used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC); used in the original Ludwig et al feeder-free culture of embryonic stem cells protocols 1-3.

High purity 17 kDa FGF2 / bFGF protein, animal-derived component free (ACDF) and carrier-protein free (CF).  This version of recombinant zebrafish FGF2 is used by the core facility at the Cambridge Stem Cell Institute, UK and historically by many of the stem cell groups based at University of Cambridge.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg may be despatched as 2 x 500µg

Buy online with secure credit card or purchase order or email orders@qkine.com
Bulk and stock reservation available


  • High purity zebrafish Danio rerio FGF2/bFGF (residues 2-154, Uniprot: B3DGE3) as described in Ludwig et al.2.

  • 17 kDa

  • Expressed in E. coli

  • Animal-free (AOF) and carrier protein-free.

  • Manufactured in our Cambridge, UK laboratories

  • Frozen in PBS

  • Prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC


Zebrafish FGF2 / bFGF Qk002 protein bioactivity lot #011

Zebrafish FGF2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells.

Cells are treated in triplicate with a serial dilution of FGF2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 0.42 ng/ml (24.7 pM). Data are from Qk002 batch #011

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340


Zebrafish FGF2 / bFGF Qk002 protein purity SDS-PAGE lot #011

FGF2 migrates as major band at 17 kDa in non-reducing (-βME) conditions and upon reduction (+βME). The higher molecular mass band at 35 kDa is a dimer that we always see in our highly purified zebrafish FGF2 protein, the presence of this does not affect biological activity.  Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.

Data from Qk002 lot #011

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Fibroblast growth factor 2 (FGF2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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  1. Stem cell researcher, University of Oxford, UK

    FGF2 for pancreatic cancer sphere systems

    Stem cell researcher, University of Oxford, UK

    We have also used the FGF2 for pancreatic cancer sphere systems and it seems to support the growth of the cells as we would expect. University of Oxford, UK.

    (0) (0)

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