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recombinant mouse/rat noggin protein (Qk033)

Mouse/rat noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.

Qkine recombinant mouse noggin protein is a highly pure 46kDa bioactive dimer for reproducible results (animal-free and carrier-protein free).

Orders are typically shipped same or next day (except Friday).
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25501005001000
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enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity mouse/rat Noggin protein (Uniprot: P97466)

  • 46 kDa (dimer)

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • tumor organoid culture

  • epithelial organoid culture (WNR media)

  • maintenance of undifferentiated embryonic stem cells

  • stem cell differentiation into neural and microglial lineages

synostoses (multiple) syndrome 1, symphalangism 1 (proximal)
  1. Krause, Carola, et al. ‘Noggin’. The International Journal of Biochemistry & Cell Biology, vol. 43, no. 4, Apr. 2011, pp. 478–81. https://doi.org/10.1016/j.biocel.2011.01.007.
  2. Fujii, Masayuki, and Toshiro Sato. ‘Somatic Cell-Derived Organoids as Prototypes of Human Epithelial Tissues and Diseases’. Nature Materials, vol. 20, no. 2, Feb. 2021, pp. 156–69. https://doi.org/10.1038/s41563-020-0754-0.

mouse, rat

bioactivity

mouse Noggin Qk033 protein bioactivity lot #104284

Noggin is a BMP inhibitor and its activity  is determined by inhibition of BMP2 (Qk007) activity in a BMP-2 responsive firefly luciferase reporter assay.

HEK293T cells are treated (in triplicate) with a serial dilution of Noggin and standard concentration of BMP2 for 6 hours.  Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity.  Data from Qk033 lot #104284 (EC50 = 1.3 nM).

purity

mouse Noggin Qk033 protein purity SDS-PAGE lot #104284

Noggin protein (Qk033) has an unusual migration in non-reduced (NR) SDS-PAGE due to the non-covalent dimer which is the active protein. Similar migration in SDS-PAGE is seen for Gremlin-1, a related BMP antagonist. The identity of the purified dimeric protein was confirmed using mass spectrometry. Upon reduction, the protein monomer migrates at 23 kDa

Purified recombinant mouse Noggin protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250

Data from Qk033 lot #104284.

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request CoA/animal-free certificate
download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

Noggin is an extracellular antagonist of BMPs (part of the larger TGFβ family); noggin binds and inhibits BMP-2, -4, -5, -7, -13, and -14 with varying affinities.1 Noggin binds these BMP ligands, preventing interaction of the ligands with their receptor, and consequently blocking downstream activation of signalling cascades.

Through its control of BMP signalling, Noggin plays a crucial role in embryonic patterning, as well as in the development of the neural tube, teeth, hair follicles, and the eye.1 In culture noggin is used in the maintenance of pluripotent stem cells, differentiation of iPSCs to neural lineages, and in a large variety of organoid culture systems including intestine, liver and lung organoids.2

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for mouse Noggin, please contact us info@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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