UK +44 (0)1223 491486      USA toll free 1-866 877 2185

Home/iPSC /ESC differentiation, HGF, human, optimised by Qkine, organoid/recombinant human HGF (NK1) protein (Qk013)


GBP: Pound sterling (£)

recombinant human HGF (NK1) protein (Qk013)

Human HGF (NK1) protein is a potent, high purity NK1 isoform of human hepatocyte growth factor (HGF) that promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α. The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.

20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.

Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.


buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email

1mg may be despatched as 2 x 500µg


  • high purity human HGF (NK1) protein (Uniprot: P14210)

  • 20 kDa

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml

Hepatopoietin-A, Scatter factor, SF

species similarity:
porcine – 95%
bovine – 93%
rat – 90%
mouse – 89%


Human HGF NK1 Qk13 protein bioactivity lot #010

Comparative bioactivity of Qk013 HGF (NK1) and a mammalian cell-expressed full-length HGF from another commercial supplier was demonstrated using a quantitative Promega serum response element luciferase reporter assay (# E1340) in transfected HEK293T cells.  EC50 of 49.6 pM and 68.4 pM respectively. Cells are treated in duplicate with a serial dilution of HGF for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.  Data from Qk013 lot #010

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340


Human HGF Qk013 protein purity SDS-PAGE lot #010

HGF NK1 migrates as major band at 20 kDa in non-reducing conditions and 18 kDa upon reduction.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+DTT, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. NB reduced samples were not boiled as the protein is sensitive to high temperatures, which causes degradation.

Data from Qk013 lot #010

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

iPSC/ESC maintenance

High purity, animal and carrier protein-free HGF protein for chemically-defined media and more reproducible scale-up

Recombinant human HGF (NK1) protein promotes efficient differentiation of human iPSCs to hepatocyte-like cells

HGF (NK1) supports human iPSC differentiation to hepatocyte-like cells. Defined media was supplemented with Qk013 HGF (NK1) during day 8-18 of the hepatocyte differentiation protocol (adapted from Heck W, et al. 2015). Cells attain characteristic hexagonal epithelial morphology. Expression of hepatic marker, HNF4α, and polarisation marker, E-Cadherin (ECAD), was detected using immunofluorescence.

Highly homogeneous expression of HNF4α in hepatocyte-like cells differentiated from iPSCs using Qk013 HGF (NK1)

Qk013 Recombinant human HGF (NK1) promotes highly homogeneous expression of HNF4α in hepatocyte-like cells differentiated from iPSCs

Growth in media supplemented with Qk013 HGF (NK1) leads to highly homogeneous expression of hepatic marker, HNF4α. iPSCs were treated with hepatocyte differentiation media supplemented with Qk013 HGF (NK1) or full-length, mammalian cell-derived HGF from another commercial source.

All experiments have been conducted by the specialist stem cell biotechnology company, Stemnovate Limited, in Cambridge, UK.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

you may also find useful

Share your thoughts!

Let us know what you think...

What others are saying

There are no contributions yet.




Your personal data will be used to support your experience throughout this website, to manage access to your account, and for other purposes described in our privacy policy.

Continue as a Guest

Don't have an account? Sign Up

Go to Top