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recombinant bovine/porcine FGF-2 (bFGF) 145aa protein (Qk040)

Bovine/porcine FGF2 protein (bFGF/basic FGF) for the development of species-specific bovine (cow) and porcine (pig) cellular agriculture protocols and veterinary research applications. Used in comparative cell culture media optimization studies alongside Qk056, the 154aa form of porcine/bovine FGF-2.

FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC)1-3  and for enhancement of proliferation in primary cell culture.

High purity 16 kDa FGF-2 / bFGF protein, animal origin-free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg may be despatched as 2 x 500µg

Buy online with secure credit card or purchase order or email orders@qkine.com
Bulk and stock reservation available

Summary

  • High purity bovine/porcine FGF-2/bFGF (Uniprot: P03969)

  • 16 kDa

  • Expressed in E. coli

  • Animal origin-free (AOF) and carrier protein-free.

  • Manufactured in our Cambridge, UK facility

  • Frozen in PBS

  • Prepare single use aliquots, add carrier protein if desired, store frozen at -20 oC or -80 oC

bioactivity

Porcine / bovine FGF-2 / bFGF Qk040 lot #104301

FGF-2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 160 pg/ml (10 pM).

Cells are treated in triplicate with a serial dilution of FGF-2 for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk040 lot #104301.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

Porcine/bovine FGF-2 / bFGF Qk040 protein purity SDS-PAGE lot #104301

Bovine/porcine FGF-2 migrates as a single major band at 16 kDa in non-reducing (NR) conditions and some dimeric protein migrating at 32 kDa. Upon reduction (R), only the 16 kDa band is visible.  No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk040 batch #104301.

further quality assays

  • mass spectrometry: single species with expected mass

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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