publications

2021

Williams, T. L., et al. ‘Human Embryonic Stem Cell-Derived Cardiomyocyte Platform Screens Inhibitors of SARS-CoV-2 Infection’. Communications Biology 4, 926 (2021). doi: 10.1038/s42003-021-02453-y.

From the lab of Anthony Davenport, University of Cambridge

FGF2

Qk002 FGF-2 / bFGF (zebrafish) Qkine protein vial
Kinoshita, M. et al. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency. Cell Stem Cell (2020) doi:10.1016/j.stem.2020.11.005.

From the lab of Austin Smith, University of Cambridge & University of Exeter

FGF2

Qk002 FGF-2 / bFGF (zebrafish) Qkine protein vial

Activin A PLUS

Qk005 Activin A PLUS (human) Qkine protein vial

In the study of embryonic stem cells, stem cells representative of naïve and primed pluripotency have been well established in the forms of embryonic stem cells (ESCs) and epiblast-derived stem cells (EpiSCs). In this study Kinoshita et al. fill the gap between early and late pluripotency in describing an intermediate state; formative stem (FS) cells. FS cells differ from both ESCs and EpiSCs, a difference beautifully exemplified by their relative contribution to chimeras. Compared with ESCs, which readily contribute to chimeras, FS chimera contribution is less frequent, and their contribution is less evenly distributed. EpiSCs on the other hand do not generally contribute to chimeras at all. FS cells were established by culturing E5.5 epiblasts, or ES cells, in N2B27 media supplemented with a low dose of Qkine Activin A alongside a Wnt inhibitor and pan-retinoic acid receptor inverse agonist. We are proud our growth factors could be part of such an exciting finding!

Masaki Kinoshita, first author, MRC Cambridge Stem Cell Institute, University of Cambridge, says:
“Formative” pluripotency exists transiently in early development and naive mouse ES cell differentiation, which cells directly respond to differentiation signals. This paper showed that formative pluripotency is now captured in culture and expands its knowledge including chimaera competency of early embryonic cells.

Andreasson, L., Evenbratt, H., Mobini, R. & Simonsson, S. Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces. J Biotechnol 325, 173–178 (2021). https://doi.org/10.1016/j.jbiotec.2020.10.030

From the lab of Stina Simonsson, University of Gothenburg

Activin A

Qk001 Activin A (human) Qkine protein vial

In embryonic development, growth factors are delivered in a highly controlled and targeted manner, however when differentiating iPSCs the real challenge is to effectively mimic these conditions. Consequently, iPSC differentiation is plagued by issues such as low efficiency and a lack of homogeneity. In their recent paper Andreasson et al. take a step towards improving the differentiation of iPSCs to definitive endoderm. The group employs gold nanoparticles to generate a gradient of immobilised Activin A – a member of the TGF-β superfamily that plays a key role in definitive endoderm development. Using this gradient, the group was able to deliver Activin A in a controlled and localised manor, resulting in more efficient differentiation. By deploying their innovative approach, the group observed a dose dependent response of the cells to Activin A, as defined by expression of differentiation markers SOX17 and GATA4. Their results indicate that it may be possible to define an optimal density of Activin A for definitive endoderm differentiation – a finding that could improve the homogeneity and speed of differentiation. This innovative study is a wonderful example of how reconsidering the way in which growth factors are delivered can lead to advances in our understanding of the precise control of stem cell differentiation and how these cells undertake their fate decisions.

2020

Borkowska, M. & Leitch, H. G. Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines. Methods Mol Biol 2214, 59–73 (2021). doi: 10.1007/978-1-0716-0958-3_5

From the lab of Harry Leitch, Imperial College London

LIF

Qk018 LIF (mouse) Qkine protein vial
Zorzan, I. et al. The transcriptional regulator ZNF398 mediates pluripotency and epithelial character downstream of TGF-beta in human PSCs. Nat Commun 11, 2364 (2020). doi: 10.1038/s41467-020-16205-9

From the lab of Graziano Martello, University of Padua

Activin A

Qk001 Activin A (human) Qkine protein vial

zebrafish FGF2

Qk002 FGF-2 / bFGF (zebrafish) Qkine protein vial
Wamaitha, S. E. et al. IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche. Nat Commun 11, 764 (2020). doi: 10.1038/s41467-020-14629-x

From the lab of Harry Leitch, Imperial College London

Activin A

Qk001 Activin A (human) Qkine protein vial

2019

Stuart, H. T. et al. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency. Cell Stem Cell 25, 388-406.e8 (2019). doi:10.1016/j.stem.2019.07.009

From the lab of José Silva, University of Cambridge

FGF2

Qk002 FGF-2 / bFGF (zebrafish) Qkine protein vial

Activin A

Qk001 Activin A (human) Qkine protein vial

LIF

Qk018 LIF (mouse) Qkine protein vial

reviewers comments available to view: Stadtfeld, M. Evaluation of Stuart et al.: Distinct Molecular Trajectories Converge to Induce Naive Pluripotency. Cell Stem Cell 25, 297–298 (2019). doi: 10.1016/j.stem.2019.08.009

2018

Blackford, S. J. I. et al. Validation of Current Good Manufacturing Practice Compliant Human Pluripotent Stem Cell-Derived Hepatocytes for Cell-Based Therapy. Stem Cells Transl Med 8, 124–137 (2019). doi:10.1002/sctm.18-0084

From the lab of Tamir Rashid, Kings College London

Activin A

Qk001 Activin A (human) Qkine protein vial