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Home/IL6 family (LIF/OSM), iPSC / ESC maintenance, mouse, mouse ES cell/recombinant mouse LIF protein (Qk018)

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recombinant mouse LIF protein (Qk018)

Mouse LIF (murine leukemia inhibitory factor) protein maintains the pluripotency and self-renewal of mouse  embryonic and induced pluripotent stem cells.

Qkine recombinant mouse LIF protein is animal-free and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression.1

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg may be despatched as 2 x 500µg

Buy online with secure credit card or purchase order or email orders@qkine.com
Bulk and stock reservation available

summary

  • high purity mouse LIF protein (Uniprot: P09056)

  • 20 kDa

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

featured applications

  • propagation of mouse ES cells

mouse/murine leukemia inhibitory factor, Differentiation-stimulating factor, D factor
  1. Mulas, C. et al. Defined conditions for propagation and manipulation of mouse embryonic stem cells. Development (Cambridge, England) 146, (2019).
  2.  Nicola, N. A. & Babon, J. J. Leukemia Inhibitory Factor (LIF). Cytokine Growth Factor Rev 26, 533–544 (2015).
  3. Graf, U., Casanova, E. A. & Cinelli, P. The Role of the Leukemia Inhibitory Factor (LIF) — Pathway in Derivation and Maintenance of Murine Pluripotent Stem Cells. Genes (Basel) 2, 280–297 (2011).
  4. Dahéron, L. et al. LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells. Stem Cells 22, 770–778 (2004).
mouse

species similarity:
human – 97%

bioactivity

Mouse LIF Qk018 protein bioactivity lot #104278 #104279 credit H Leitch, Imperial College, London, UK

Recombinant mouse LIF protein (animal-free) supports mouse embryonic stem cell colony formation and has been benchmarked against LIF supplement from Supplier A in chemically-defined feeder-free culture.

Mouse LIF (Qk018) support mouse ES cell propagation in chemically-defined, feeder-free iPSC culture. Cultures are dissociated to a single cell suspension and plated at very low (clonal) density in defined media containing Qk018 recombinant mouse LIF or mouse LIF supplement from another supplier (Supplier A). The number of colonies that formed was determined after 4-5 days.

Our thanks to Leitch lab, MRC London Institute of Medical Sciences, Imperial College for mouse ES cell data detailed above and discussion.

Data from Qk018 lot #104278 and #104279

purity

Mouse LIF Qk018 protein purity SDS-PAGE lot #104278

Mouse LIF protein (Qk018) migrates as a single band at 20 kDa in non-reducing (NR) and upon reduction (R) with β-mercaptoethanol.

Purified recombinant mouse LIF protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk018 lot #104278.

further quality assays

  • mass spectrometry: single species with expected mass

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

LIF is a pleiotrophic factor that belongs to the IL-6 superfamily of cytokines. Acting through a heterodimeric receptor of LIFR (gp190) and gp130, LIF activates a number of cellular pathways including the JAK/STAT, PI3K and MAPK pathways.2 LIF signalling is essential for maintenance of murine pluripotency.3 Human LIF can be used for the maintenance of mouse embryonic stem cells, however mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive on human cells.4

mouse LIF vs ESGRO

confused about the difference between mLIF and ESGRO? read our blog

or read more about pluripotency

additional resources

Technote: mLIF vs ESGRO

Customer & collaborator data

Flow cytometry analysis of Nanog-reporter positive mouse ES cells cultured using Qk018 mouse LIF vs. ESGRO

Flow cytometry was used to compare the percentage of Nanog-reporter positive mouse ES cells cultured in a chemically-defined, feeder-free iPSC culture media containing either Qk018 mouse LIF or ESGRO. Our results show that Nanog expression in mouse ES cells cultured with Qk018 mouse LIF was comparable to, if not potentially better than, ESGRO.

Experiments performed by Harry Leitch (MA MB BChir PhD MRCPCH), MRC London Institute of Medical Sciences, Imperial College.

MLIF

The LIF we tested from Qkine has shown excellent quality in maintaining mouse embryonic stem cells derived from precious samples and maintaining them in a pluripotent state.

Experiments conducted by Dylan Liabeuf. 

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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