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Mouse LIF (murine leukemia inhibitory factor) protein supports the derivation, propagation and expansion of mouse embryonic stem cells (ESC), and maintenance of pluripotency. LIF protein increases the robustness of mouse embryonic stem cell culture in feeder or feeder-free chemically defined culture systems.
Qkine recombinant mouse LIF protein is animal-free and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression (1).
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1mg may be despatched as 2 x 500µg
high purity mouse LIF protein (Uniprot: P09056)
expressed in E. coli
animal-free (AOF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
lyophilized from acetonitrile, TFA
resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC
propagation of mouse ES cells
Recombinant mouse LIF protein (animal-free) supports mouse embryonic stem cell colony formation and has been benchmarked against LIF supplement from Supplier A in chemically-defined feeder-free culture.
Mouse LIF (Qk018) support mouse ES cell propagation in chemically-defined, feeder-free iPSC culture. Cultures are dissociated to a single cell suspension and plated at very low (clonal) density in defined media containing Qk018 recombinant mouse LIF or mouse LIF supplement from another supplier (Supplier A). The number of colonies that formed was determined after 4-5 days.
Our thanks to Leitch lab, MRC London Institute of Medical Sciences, Imperial College for mouse ES cell data detailed above and discussion.
Data from Qk018 lot #104278 and #104279
mouse LIF protein (Qk018) migrates as a single band at 20 kDa in non-reducing (NR) and upon reduction (R) with β-mercaptoethanol.
Purified recombinant mouse LIF protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk018 lot #104278.
further quality assays
mass spectrometry: single species with expected mass
endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Our thanks to Leitch lab, MRC London Institute of Medical Sciences, Imperial College for mouse ES cell data and discussion.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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– October 28, 2020
Flow cytometry was used to compare the percentage of Nanog-reporter positive mouse ES cells cultured in a chemically-defined, feeder-free iPSC culture media containing either Qk018 mouse LIF or ESGRO. Our results show that Nanog expression in mouse ES cells cultured with Qk018 mouse LIF was comparable to, if not potentially better than, ESGRO.
Harry Leitch (MA MB BChir PhD MRCPCH), MRC London Institute of Medical Sciences, Imperial College.
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