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Mouse LIF (murine leukemia inhibitory factor) protein maintains the pluripotency and self-renewal of mouse embryonic and induced pluripotent stem cells.
Qkine recombinant mouse LIF protein is animal-free and carrier-protein free for highly reproducible results. Bioactivity was tested by colony formation assay and determination of Nanog expression.1
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High purity mouse LIF protein (Uniprot: P09056)
>97%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Propagation of mouse ES cells
Human – 97%
Recombinant human BMP4
Recombinant human LIF
Recombinant mouse LIF protein (animal-free) supports mouse embryonic stem cell colony formation and has been benchmarked against LIF supplement from Supplier A in chemically-defined feeder-free culture.
Mouse LIF (Qk018) support mouse ES cell propagation in chemically-defined, feeder-free iPSC culture. Cultures are dissociated to a single cell suspension and plated at very low (clonal) density in defined media containing Qk018 recombinant mouse LIF or mouse LIF supplement from another supplier (Supplier A). The number of colonies that formed was determined after 4-5 days.
Our thanks to Leitch lab, MRC London Institute of Medical Sciences, Imperial College for mouse ES cell data detailed above and discussion.
Data from Qk018 lot #104278 and #104279
Mouse LIF protein (Qk018) migrates as a single band at 20 kDa in non-reducing (NR) and upon reduction (R) with β-mercaptoethanol.
Purified recombinant mouse LIF protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk018 lot #104278.
Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
LIF is a pleiotrophic factor that belongs to the IL-6 superfamily of cytokines. Acting through a heterodimeric receptor of LIFR (gp190) and gp130, LIF activates a number of cellular pathways including the JAK/STAT, PI3K and MAPK pathways.2 LIF signalling is essential for maintenance of murine pluripotency.3 Human LIF can be used for the maintenance of mouse embryonic stem cells, however mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive on human cells.4
Confused about the difference between mLIF and ESGRO?
Read our blog
or read more about pluripotency
Flow cytometry was used to compare the percentage of Nanog-reporter positive mouse ES cells cultured in a chemically-defined, feeder-free iPSC culture media containing either Qk018 mouse LIF or ESGRO. Our results show that Nanog expression in mouse ES cells cultured with Qk018 mouse LIF was comparable to, if not potentially better than, ESGRO.
Experiments performed by Harry Leitch (MA MB BChir PhD MRCPCH), MRC London Institute of Medical Sciences, Imperial College.
The LIF we tested from Qkine has shown excellent quality in maintaining mouse embryonic stem cells derived from precious samples and maintaining them in a pluripotent state.
Experiments conducted by Dylan Liabeuf.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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