Recombinant mouse LIF protein (animal-free) supports mouse embryonic stem cell colony formation and has been benchmarked against LIF supplement from Supplier A in chemically-defined feeder-free culture.
Mouse LIF (Qk018) support mouse ES cell propagation in chemically-defined, feeder-free iPSC culture. Cultures are dissociated to a single cell suspension and plated at very low (clonal) density in defined media containing Qk018 recombinant mouse LIF or mouse LIF supplement from another supplier (Supplier A). The number of colonies that formed was determined after 4-5 days.
Our thanks to Leitch lab, MRC London Institute of Medical Sciences, Imperial College for mouse ES cell data detailed above and discussion.
Data from Qk018 lot #104278 and #104279