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Human LIF (leukemia inhibitory factor) protein suppresses the differentiation of embryonic stem cells (ESCs) and is widely used in ESC and iPSC culture (1). Human LIF can be used in the maintenance of both murine and human ESCs in feeder or feeder-free chemically defined culture systems (2).
LIF is a pleiotrophic factor that belongs to the IL-6 superfamily of cytokines. Acting through a heterodimeric receptor of gp130 and gp190, LIF can activate a number of cellular pathways including the JAK/STAT, PI3K and MAPK pathways (3).
Qkine human LIF protein is high purity and animal and carrier-protein free for reproducible results.
Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.
buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email firstname.lastname@example.org
1mg may be despatched as 2 x 500µg
high purity human LIF protein (Uniprot: P15018)
cross-reactivity: human, mouse (murine), rat
expressed in E. coli.
animal-free (AOF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
lyophilized from acetonitrile, TFA
resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC
propagation of ES cells in feeder or feeder-free chemically defined culture systems
human LIF can be used in the maintenance of both murine and human ESCs
LIF growth factor bioactivity is determined in a LIF responsive firefly luciferase reporter assay, EC50 = 0.28 ng/ml (14.1 pM).
HEK293T cells are treated (in triplicate) with a serial dilution of LIF overnight. Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity. Data from Qk036 lot #104293.
Human LIF protein (Qk036) migrates as a single band at 20 kDa in non-reducing (NR) and upon reduction (R)
Purified recombinant human LIF protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk036 lot #104293.
further quality assays
mass spectrometry: single species with expected mass
analytical reversed-phase: single sharp peak
endotoxin: <0.005 EU/μg protein (below level of detection)
recovery from stock vial >95%
We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback, please submit a review or contact our team email@example.com
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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