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Home/iPSC / ESC maintenance, LIFs, mouse ES cell, neural/recombinant human LIF protein (Qk036)

recombinant human LIF protein (Qk036)

Recombinant human LIF (leukemia inhibitory factor) protein suppresses the differentiation of embryonic stem cells (ESCs) and is widely used in ESC and iPSC culture (1).  Human LIF can be used in the maintenance of both murine and human ESCs in feeder or feeder-free chemically defined culture systems (2)

LIF is a pleiotrophic factor that belongs to the IL-6 superfamily of cytokines. Acting through a heterodimeric receptor of gp130 and gp190, LIF can activate a number of cellular pathways including the JAK/STAT, PI3K and MAPK pathways (3).

Qkine human LIF protein is high purity and animal and carrier-protein free for reproducible results.

Orders are typically shipped same or next day (except Friday). Easy world-wide shipping.

25501005001000
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buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity human LIF protein (Uniprot: P15018)

  • cross-reactivity: human, mouse (murine), rat

  • 20 kDa

  • expressed in E. coli. 

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

leukemia inhibitory factor, Differentiation-stimulating factor, D factor, melanoma-derived LPL inhibitor (MLPLI)
1.
Graf, U., Casanova, E. A. & Cinelli, P. The Role of the Leukemia Inhibitory Factor (LIF) — Pathway in Derivation and Maintenance of Murine Pluripotent Stem Cells. Genes (Basel) 2, 280–297 (2011).
2.
Dahéron, L. et al. LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells. Stem Cells 22, 770–778 (2004).
3.
Nicola, N. A. & Babon, J. J. Leukemia Inhibitory Factor (LIF). Cytokine Growth Factor Rev 26, 533–544 (2015).

bioactivity

Human LIF Qk036 protein bioactivity lot #14293

LIF growth factor bioactivity is determined in a LIF responsive firefly luciferase reporter assay, EC50 = 0.28 ng/ml (14.1 pM).

HEK293T cells are treated (in triplicate) with a serial dilution of LIF overnight. Firefly luciferase activity is measured and normalised to the control Renilla luciferase activity. Data from Qk036 lot #104293.

purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

Human LIF protein (Qk036) migrates as a single band at 20 kDa in non-reducing (NR) and upon reduction (R)

Purified recombinant human LIF protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk036 lot #104293.

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak 

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial >95%

request lot-specific CoA

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

organoid
iPSC/ESC maintenance
neural
hepatocyte
pancreatic
endodermal
mesodermal
epithelial
mesenchymal
hematopoietic

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback, please submit a review or contact our team support@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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