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recombinant human/mouse/rat Activin A PLUS protein (Qk005)
Human/mouse/rat activin A PLUS protein is an optimised biologically active truncation of the mature domain of human Activin A protein. The EC50 and activity in stem cell culture of activin A PLUS is equivalent to the full mature domain activin A.
High purity 24 kDa dimer comprising truncated mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF). Recombinant activin A PLUS is designed to be manufactured at scale for cost-effective, large-scale stem cell culture applications.
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com
1mg may be despatched as 2 x 500µg
summary
high purity optimized mature domain of human activin A (Uniprot: P08476) – particularly suitable for bulk and scale-up stem cell culture applications
cross-reactivity: human, mouse (murine), rat
24 kDa (dimer)
expressed in E. coli
animal-free (AOF) and carrier protein-free
manufactured in our Cambridge, UK laboratories
lyophilized from acetonitrile, TFA
resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC
featured applications
large scale stem cell expansion
1. Harrington, A. E. et al. Structural basis for the inhibition of activin signalling by follistatin. EMBO J. 25, 1035–1045 (2006).
2. Wang, X., Fischer, G. & Hyvönen, M. Structure and activation of pro-activin A. (2016). doi:10.1038/ncomms12052
3. Pauklin, S. & Vallier, L. Activin/Nodal signalling in stem cells. Development 142, 607–19 (2015).
4. D’Amour, K. A. et al. Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. Biotechnol. 23, 1534–1541 (2005).
bioactivity
Activin A PLUS activity is determined using an activin-responsive firefly luciferase reporter in HEK293T cells. in triplicate) with a serial dilution of activin A for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.
EC50 = 5.6 pM . EC50 is within the expected range of 6 ± 2 pM. Data from Qk005 lot #010.
purity
Activin A PLUS migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk005 lot #010.
further quality assays
mass spectrometry: single species with expected mass
analytical reversed-phase: single sharp peak
endotoxin: <0.005 EU/μg protein (below level of detection)
- recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
research areas
protein background
Activin A is a member of the TGFβ superfamily of growth factors used in stem cell differentiation and maintenance. Activin A Plus, an engineered optimized form of Activin A, incorporates an N-terminal truncation to remove a disulfide-linked extension. The resulting protein can be manufactured at extremely consistent high yield with no observed changes in bioactivity compared to the wild-type. This form is particularly suitable for large scale stem cell culture processes.
Activin A is involved in regulation of embryogenesis, development of the reproductive system, wound healing and regulation of immune responses in vivo. The activity of Activin A is regulated by the high-affinity inhibitor, follistatin1, and inhibins. Activins are disulfide-linked homo- and heterodimers of four inhibin β chains. The best characterized are Activin A and Activin B, homodimers of inhibin βA and inhibin βB respectively. Activins, like all other members in the TGF-β superfamily, are synthesized as larger precursors consisting of an N-terminal signal peptide, a pro-domain of 250–350 residues and a highly conserved mature domain. The pro-domain, which is cleaved off in the mature protein, has important roles in the biosynthesis, stabilization, transportation and signalling of the growth factors2.
product documents
We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for human Activin A PLUS, please contact us info@qkine.com”
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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