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recombinant human/mouse/rat activin A protein (Qk001)

Human/mouse/rat activin A protein is a member of the TGFβ superfamily of growth factors used for maintenance of pluripotency in many induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) lines, and to induce stem cell differentiation into endoderm and other cell fates.  Extensively validated for its reproducibility and as used at the Cambridge Stem Cell Institute.

High purity 26 kDa dimer comprising mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF).  Rigorously benchmarked against other commercial sources.  We’re proud of it …  what do you think?    A commentary by Qkine’s founder Marko Hyvönen.

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enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity mature domain of human activin A protein, residues 311-426, (Uniprot: P08476)

  • 26 kDa (dimer)

  • expressed in E. coli

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • induced pluripotent and embryonic stem cell differentiation and maintenance

  • regulated by the high-affinity inhibitor, follistatin1

bioactivity

Human Activin Qk001 protein bioactivity lot #011

Activin A protein activity is determined using an activin-responsive firefly luciferase reporter in HEK293T cells.  EC50 = 6.1 pM .  EC50 is within the expected range of 6 ± 2 pM.  Data from Qk001 lot #011

purity

Human Activin A Qk001 protein purity SDS-PAGE lot #011

ActA migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R).  No contaminating protein bands are visible.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.  Data from Qk001 lot #011.

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial:  >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

organoid
iPSC/ESC maintenance
neural
hepatocyte
pancreatic
endodermal
mesodermal
epithelial
mesenchymal
hematopoietic

protein background

Human Activin A protein is a member of the TGFβ superfamily of growth factors involved in stem cell differentiation and maintenance, regulation of embryogenesis, development of the reproductive system, wound healing and regulation of immune responses. The activity of Activin A protein is regulated by the high-affinity inhibitor, follistatin1, and inhibins.

Activins are disulfide-linked homo- and heterodimers of four inhibin β chains. The best characterized are Activin A and Activin B, homodimers of inhibin βA and inhibin βB respectively. Activins, like all other members in the TGF-β superfamily, are synthesized as larger precursors consisting of an N-terminal signal peptide, a pro-domain of 250–350 residues and a highly conserved mature domain. The pro-domain, which is cleaved off in the mature protein, has important roles in the biosynthesis, stabilization, transportation and signalling of the growth factors2.

Recombinant Activin A is used for maintenance of pluripotency in many human induced-pluripotent stem cell and human embryonic stem cell lines3, and to induce stem cell differentiation into endoderm4 and other cell fates.

Our recombinant Activin A protein has been extensively validated and benchmarked with other suppliers’ proteins in stem cell culture and other assays. View the results of this analysis and a commentary by Qkine’s founder Marko Hyvönen.

Blackford SJI, Ng SS, Segal JM, King AJF, Austin AL, Kent D, Moore J, Sheldon M, Ilic D, Dhawan A, Mitry RR, Rashid ST: Validation of Current Good Manufacturing Practice Compliant Human Pluripotent Stem Cell-Derived Hepatocytes for Cell-Based Therapy.. In: Stem Cells Transl Med, 2018.

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for human Activin A, please contact us info@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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  1. Stem cell researcher, University of Oxford, UK

    Qkine Activin A works nicely for human pluripotent stem cell differentiation to endoderm

    Stem cell researcher, University of Oxford, UK

    We are using Qkine Activin A and it works nicely for human pluripotent stem cell differentiation to endoderm. University of Oxford, UK.

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