Qk001 Activin A (human) Qkine protein vial

Human Activin A Qk001 protein purity SDS-PAGE lot #011

recombinant human/mouse/rat/bovine/porcine activin A protein (Qk001)

Human/mouse/rat/bovine/porcine activin A protein is a member of the TGFβ superfamily of growth factors used for maintenance of pluripotency in many induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) lines, and to induce stem cell differentiation into endoderm and other cell fates. Extensively validated for its reproducibility and as used at the Cambridge Stem Cell Institute.

High purity 26 kDa dimer comprising mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF). Rigorously benchmarked against other commercial sources. We’re proud of it … what do you think? A commentary by Qkine’s founder Marko Hyvönen.

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1mg may be despatched as 2 x 500µg

summary

high purity mature domain of human activin A protein, residues 311-426, (Uniprot: P08476)
26 kDa (dimer)
expressed in E. coli
animal-free (AOF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
lyophilized from acetonitrile, TFA
resuspend in 10mM HCl at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

induced pluripotent and embryonic stem cell differentiation and maintenance
regulated by the high-affinity inhibitor, follistatin¹

alternative protein names

ActA, Activin beta-A chain, Erythroid differentiation protein (EDP), Follicle-Stimulating Hormone (FSH) releasing protein (FRP), INHBA, Inhibin beta A, Inhibin beta 1

background references

1. Harrington, A. E. et al. Structural basis for the inhibition of activin signalling by follistatin. EMBO J. 25, 1035–1045 (2006).
2. Wang, X., Fischer, G. & Hyvönen, M. Structure and activation of pro-activin A. (2016). doi:10.1038/ncomms12052
3. Pauklin, S. & Vallier, L. Activin/Nodal signalling in stem cells. Development 142, 607–19 (2015).
4. D’Amour, K. A. et al. Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. Biotechnol. 23, 1534–1541 (2005).

species reactivity

human, mouse, rat, bovine, porcine

bioactivity

Activin A protein activity is determined using an activin-responsive firefly luciferase reporter in HEK293T cells. EC50 = 11.9 nM Data from #104276. n=3

purity

ActA migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible.

Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk001 lot #011.

further quality assays

mass spectrometry: single species with expected mass
analytical reversed-phase: single sharp peak
endotoxin: <0.005 EU/μg protein (below level of detection)
recovery from stock vial: >95%

request batch-specific CoA

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

research areas

protein background

Human Activin A protein is a member of the TGFβ superfamily of growth factors involved in stem cell differentiation and maintenance, regulation of embryogenesis, development of the reproductive system, wound healing and regulation of immune responses. The activity of Activin A protein is regulated by the high-affinity inhibitor, follistatin¹, and inhibins.

Activins are disulfide-linked homo- and heterodimers of four inhibin β chains. The best characterized are Activin A and Activin B, homodimers of inhibin βA and inhibin βB respectively. Activins, like all other members in the TGF-β superfamily, are synthesized as larger precursors consisting of an N-terminal signal peptide, a pro-domain of 250–350 residues and a highly conserved mature domain. The pro-domain, which is cleaved off in the mature protein, has important roles in the biosynthesis, stabilization, transportation and signalling of the growth factors².

Recombinant Activin A is used for maintenance of pluripotency in many human induced-pluripotent stem cell and human embryonic stem cell lines³, and to induce stem cell differentiation into endoderm⁴ and other cell fates.

Our recombinant Activin A protein has been extensively validated and benchmarked with other suppliers’ proteins in stem cell culture and other assays. View the results of this analysis and a commentary by Qkine’s founder Marko Hyvönen.

Blackford SJI, Ng SS, Segal JM, King AJF, Austin AL, Kent D, Moore J, Sheldon M, Ilic D, Dhawan A, Mitry RR, Rashid ST: Validation of Current Good Manufacturing Practice Compliant Human Pluripotent Stem Cell-Derived Hepatocytes for Cell-Based Therapy.. In: Stem Cells Transl Med, 2018.

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback for human Activin A, please contact us info@qkine.com”

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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What others are saying

Qkine Activin A works nicely for human pluripotent stem cell differentiation to endoderm

Stem cell researcher, University of Oxford, UK – July 20, 2018

We are using Qkine Activin A and it works nicely for human pluripotent stem cell differentiation to endoderm. University of Oxford, UK.

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