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Home/cellular agriculture, hematopoietic, human, IL6 family (LIF/OSM), iPSC / ESC maintenance, neural/recombinant human oncostatin M (OSM) protein (Qk049)

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recombinant human oncostatin M (OSM) protein (Qk049)

Human OSM (oncostatin M) protein is used in the differentiation of human pluripotent stem cells into hepatocyte-like cells. OSM also plays roles in osteogenesis and neurogenesis, and is an important regulator of the hematopoietic stem cell niche in the bone marrow.

Qkine human oncostatin M protein is high purity and animal and carrier-protein free for reproducible results.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

25501005001000
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buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com

1mg may be despatched as 2 x 500µg

summary

  • high purity human OSM protein (Uniprot: P13725)

  • 22 kDa

  • expressed in E. coli. 

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

handling and storage FAQ

featured applications

  • maturation of hepatoblasts into hepatocyte like cells

Oncostatin M, OSM

human

species similarity:
bovine – 62%
porcine – 54%
rat – 45%
mouse – 45%

NB: although the sequence identity is low, human OSM has been shown to be active on mouse ES cells

bioactivity

Human LIF Qk036 protein bioactivity lot #14293

OSM activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 0.75 ng/ml (34 pM).

Cells are treated in triplicate with a serial dilution of OSM for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk049 lot #104336.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

OSM migrates as a single band at 20 kDa in non-reducing (NR) conditions and 22 kDa upon reduction (R).  The slight band under the main band in the reduced sample is likely an artefact of the reduction protocol. No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk049 batch #104336.

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak 

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial >95%

request CoA/animal-free certificate
download MSDS

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

Oncostatin M (OSM) is a 30 kDa secreted cytokine. OSM is a member of the Interleukin¬6 family, which includes IL¬11, CNTF and Cardiotrophin¬ 1. Human OSM is produced in cells as a 252 amino acid pre-pro-protein. The signal peptide and the C- terminal domain are proteolytically cleaved to generate the mature 195 aa form.
Cytokines of the IL6 family bind a common receptor subunit, gp130. Unlike other family members, OSM binds gp130 with low affinity and requires recruitment of either LIF R α or OSM R β to form the OSM receptor type I or OSM receptor type II respectively. OSM has many physiological roles and is used in several iPSC and ESC protocols. OSM has essential functions in inflammation, neuroprotection, hematopoiesis, metabolism, early development, tumorigenesis and epithelial cell growth (Richards et al. 2013).
Human OSM shares 45% aa sequence homology with mouse and rat OSM, 54% bovine and 62% porcine. There is evidence that human OSM is active on mouse cells.
In stem cell applications, animal-free oncostatin M is used to promote maturation of hepatoblasts into hepatocyte-like cells for liver regenerative medicine research and drug and toxicology assay development (Hannan et al. 2013, Hay et al. 2008, Tan et al. 2021).
Recent evidence suggests OSM can maintain naïve mouse embryonic stem cells in the absence of LIF, although the significance and general application of this observation is to be established (Ye, 2021).
OSM also has uses in cultured meat applications, hematopoietic stem cell differentiation and basic research.

Hannan, Nicholas R. F., Charis-Patricia Segeritz, Thomas Touboul, and Ludovic Vallier. ‘Production of Hepatocyte-like Cells from Human Pluripotent Stem Cells’. Nature Protocols 8, no. 2 (February 2013): 430–37. https://doi.org/10.1038/nprot.2012.153.
Hay, David C., Debiao Zhao, Judy Fletcher, Zoë A. Hewitt, Doris McLean, Alai Urruticoechea-Uriguen, James R. Black, et al. ‘Efficient Differentiation of Hepatocytes from Human Embryonic Stem Cells Exhibiting Markers Recapitulating Liver Development in Vivo’. Stem Cells (Dayton, Ohio) 26, no. 4 (April 2008): 894–902. https://doi.org/10.1634/stemcells.2007-0718.
Richards, Carl D. ‘The Enigmatic Cytokine Oncostatin M and Roles in Disease’. ISRN Inflammation 2013 (8 December 2013): e512103. https://doi.org/10.1155/2013/512103.
Tan, Lay Shuen, Resilind Su Ern Chew, Natasha Hui Jin Ng, and Adrian Kee Keong Teo. ‘Protocol for the Generation of Pancreatic and Hepatic Progenitors from Human Pluripotent Stem Cells for Gene Regulatory Assays’. STAR Protocols 2, no. 2 (18 June 2021): 100471. https://doi.org/10.1016/j.xpro.2021.100471.
West, Nathaniel R., Benjamin M. J. Owens, and Ahmed N. Hegazy. ‘The Oncostatin M-Stromal Cell Axis in Health and Disease’. Scandinavian Journal of Immunology 88, no. 3 (2018): e12694. https://doi.org/10.1111/sji.12694.
Ye, Xiaoying, Chenglei Tian, Linlin Liu, Guofeng Feng, Kairang Jin, Haiying Wang, Jiyu Chen, and Lin Liu. ‘Oncostatin M Maintains Naïve Pluripotency of MESCs by Tetraploid Embryo Complementation (TEC) Assay’. Frontiers in Cell and Developmental Biology 9 (2021): 1341. https://doi.org/10.3389/fcell.2021.675411.

We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback, please submit a review or contact our team support@qkine.com

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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