Recombinant human/bovine/porcine IGF-1 protein (Qk047)

Name: Recombinant human/bovine/porcine IGF-1 protein (Qk047)
SKU: Qk047
Availability: InStock

Human IGF-1 protein (IGF-I), insulin-like growth factor 1, is used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin¹. IGF-1 promotes the proliferation of many cell types, including mesenchymal stem cells, in vitro.

Highly pure and bioactive 8 kDa IGF-1 protein, animal-free (AF) and carrier-protein free (CF)

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg may be despatched as 2 x 500µg

Buy online with secure credit card or purchase order or email orders@qkine.com
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Description

Summary

High purity human IGF-1 protein (Uniprot:P05019)
>97%, by SDS-PAGE quantitative densitometry
8 kDa
Expressed in E. coli.
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Handling and storage FAQ

Featured applications

Maintenance of pluripotency in human iPSC
Cell culture media optimisation and replacement of insulin in defined media

Alternative protein names

Insulin-like Growth Factor-I, Somatamedin-C, Mechano Growth Factor (MGF)

Background references

1. Wang, Linlin, et al. ‘Self-Renewal of Human Embryonic Stem Cells Requires Insulin-like Growth Factor-1 Receptor and ERBB2 Receptor Signaling’. Blood, vol. 110, no. 12, Dec. 2007, pp. 4111–19.

2. Laron, Z. ‘Insulin-like Growth Factor 1 (IGF-1): A Growth Hormone’. Molecular Pathology, vol. 54, no. 5, Oct. 2001, pp. 311–16.

Species reactivity

human
porcine (pig)
bovine (cow)

Frequently used together

Recombinant human activin A protein
Recombinant zebrafish FGF-2 protein
Recombinant human FGF-2 145aa protein
Recombinant human TGFβ1 PLUS protein
Recombinant human NRG-1 protein

Alternative

Recombinant human IGF-1 LR3 protein

Bioactivity

IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM).

Cells are treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

IGF-1 protein migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk047 batch #104331.

Further quality assays

Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial >95%

Request CoA/animal-free certificate

Download MSDS

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Insulin-like growth factor 1 (IGF-1 or IGF-I) is a protein with a similar structure to insulin and is used frequently to replace or reduce the requirement for insulin in cell culture media. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic and lung. IGF-1 activates numerous signalling pathways, including PI3K/AKT and ERK1/2 signalling, through binding the IGF-1 receptor.²

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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