UK +44 (0)1223 491486 USA toll free 1-866 877 2185
Register here for our next webinar
Human IGF-1 protein (IGF-I), insulin-like growth factor 1, is used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin1. IGF-1 promotes the proliferation of many cell types, including mesenchymal stem cells, in vitro.
Highly pure and bioactive 8 kDa IGF-1 protein, animal-free (AF) and carrier-protein free (CF)
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1mg may be despatched as 2 x 500µg
Buy online with secure credit card or purchase order or email email@example.com
Bulk and stock reservation available
High purity human IGF-1 protein (Uniprot:P05019)
>97%, by SDS-PAGE quantitative densitometry
Expressed in E. coli.
Animal-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Maintenance of pluripotency in human iPSC
Cell culture media optimisation and replacement of insulin in defined media
1. Wang, Linlin, et al. ‘Self-Renewal of Human Embryonic Stem Cells Requires Insulin-like Growth Factor-1 Receptor and ERBB2 Receptor Signaling’. Blood, vol. 110, no. 12, Dec. 2007, pp. 4111–19.
2. Laron, Z. ‘Insulin-like Growth Factor 1 (IGF-1): A Growth Hormone’. Molecular Pathology, vol. 54, no. 5, Oct. 2001, pp. 311–16.
Recombinant human activin A protein
Recombinant zebrafish FGF-2 protein
Recombinant human FGF-2 145aa protein
Recombinant human TGFβ1 PLUS protein
Recombinant human NRG-1 protein
Recombinant human IGF-1 LR3 protein
IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM).
Cells are treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331.
*Promega pGL4.33[luc2P/SRE/Hygro] #E1340
IGF-1 protein migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk047 batch #104331.
Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial >95%
We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Insulin-like growth factor 1 (IGF-1 or IGF-I) is a protein with a similar structure to insulin and is used frequently to replace or reduce the requirement for insulin in cell culture media. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic and lung. IGF-1 activates numerous signalling pathways, including PI3K/AKT and ERK1/2 signalling, through binding the IGF-1 receptor.2
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
Enter a URL (optional)
Choose a file
You must be logged in to join the discussion.
Show everythingShow all reviewsShow all questionsShow all photosShow helpful positive reviewsShow helpful negative reviewsShow unanswered questions
There are no contributions yet.
Username or email address *
Lost your password?
Email address *
Email address *
Already have an account? Log In
Don't have an account? Sign Up
Continue as a Guest
we respect your time and privacy
© 2023 Qkine Ltd. Company number 10470662 registered in England and Wales. All rights reserved