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recombinant human/bovine/porcine IGF-1 protein (Qk047)
Human IGF-1 (IGF-I), insulin-like growth factor 1, protein is used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin1. IGF-1 promotes the proliferation of many cell types, including mesenchymal stem cells, in vitro.
Highly pure and bioactive 8 kDa IGF-1 protein, animal-free (AF) and carrier-protein free (CF)
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
buy online with secure credit card or purchase order
enquiries, bulk and stock reservation, please email orders@qkine.com
1mg may be despatched as 2 x 500µg
summary
high purity human IGF-1 protein (Uniprot:P05019)
8 kDa
expressed in E. coli.
animal-free (AOF) and carrier protein-free.
manufactured in our Cambridge, UK laboratories
lyophilized from acetonitrile, TFA
resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC
featured applications
cell culture media optimisation and replacement of insulin in defined media
1. Wang, Linlin, et al. ‘Self-Renewal of Human Embryonic Stem Cells Requires Insulin-like Growth Factor-1 Receptor and ERBB2 Receptor Signaling’. Blood, vol. 110, no. 12, Dec. 2007, pp. 4111–19.
2. Laron, Z. ‘Insulin-like Growth Factor 1 (IGF-1): A Growth Hormone’. Molecular Pathology, vol. 54, no. 5, Oct. 2001, pp. 311–16.
porcine (pig)
bovine (cow)
alternative
bioactivity
IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM).
Cells are treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331.
purity
IGF-1 migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible.
Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk047 batch #104331.
further quality assays
mass spectrometry: single species with expected mass
analytical reversed-phase: single sharp peak
endotoxin: <0.005 EU/μg protein (below level of detection)
recovery from stock vial >95%
We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
protein background
Insulin-like growth factor 1 (IGF-1 or IGF-I) is a protein with a similar structure to insulin and is used frequently to replace or reduce the requirement for insulin in cell culture media. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic and lung. IGF-1 activates numerous signalling pathways, including PI3K/AKT and ERK1/2 signalling, through binding the IGF-1 receptor.2
We welcome all feedback and specialist collaborator data on all our products. If you would like to provide application-specific feedback, please submit a review or contact our team support@qkine.com
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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