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Home/epithelial, hematopoietic, human, IGF family, iPSC / ESC maintenance, neural/recombinant human/bovine/porcine IGF-1 protein (Qk047)

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recombinant human/bovine/porcine IGF-1 protein (Qk047)

Human IGF-1 (IGF-I), insulin-like growth factor 1, protein is used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin1. IGF-1 promotes the proliferation of many cell types, including mesenchymal stem cells, in vitro.

Highly pure and bioactive 8 kDa IGF-1 protein, animal-free (AF) and carrier-protein free (CF)

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg may be despatched as 2 x 500µg

Buy online with secure credit card or purchase order or email orders@qkine.com
Bulk and stock reservation available

summary

  • high purity human IGF-1 protein (Uniprot:P05019)

  • 8 kDa

  • expressed in E. coli. 

  • animal-free (AOF) and carrier protein-free.

  • manufactured in our Cambridge, UK laboratories

  • lyophilized from acetonitrile, TFA

  • resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20 oC or -80 oC

featured applications

Insulin-like Growth Factor-I, Somatamedin-C, Mechano Growth Factor (MGF)

1. Wang, Linlin, et al. ‘Self-Renewal of Human Embryonic Stem Cells Requires Insulin-like Growth Factor-1 Receptor and ERBB2 Receptor Signaling’. Blood, vol. 110, no. 12, Dec. 2007, pp. 4111–19.

2. Laron, Z. ‘Insulin-like Growth Factor 1 (IGF-1): A Growth Hormone’. Molecular Pathology, vol. 54, no. 5, Oct. 2001, pp. 311–16.

human
porcine (pig)
bovine (cow)

frequently used together

recombinant human activin A protein
recombinant zebrafish FGF-2 protein
recombinant human FGF-2 145aa protein
recombinant human TGFβ1 PLUS protein
recombinant human NRG-1 protein

alternative

recombinant human IGF-1 LR3 protein

bioactivity

Human LIF Qk036 protein bioactivity lot #14293

IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM).

Cells are treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331.

*Promega pGL4.33[luc2P/SRE/Hygro] #E1340

purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

IGF-1 migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R).  No contaminating protein bands are visible.

Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.  Data from Qk047 batch #104331.

further quality assays

  • mass spectrometry: single species with expected mass

  • analytical reversed-phase: single sharp peak 

  • endotoxin: <0.005 EU/μg protein (below level of detection)

  • recovery from stock vial >95%

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

protein background

Insulin-like growth factor 1 (IGF-1 or IGF-I) is a protein with a similar structure to insulin and is used frequently to replace or reduce the requirement for insulin in cell culture media.  IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic and lung. IGF-1 activates numerous signalling pathways, including PI3K/AKT and ERK1/2 signalling, through binding the IGF-1 receptor.2

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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Reconstitution selector

Recommended buffer:

10 mM HCl
from June 2021 we will send this buffer to you with your product to make reconstitution easy
H20
this protein is provided frozen, dilution can be performed with your choice of media or buffer

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