- Recombinant human vascular endothelial growth factor 165 (also known as VEGF165/ VEGF-165/ VEGF-A/ VEGF165) protein is widely used in culturing primary endothelial cells, such as human umbilical vein endothelial cells.VEGF165 is commonly used with human-induced pluripotent stem cells or embryonic stem cells-derived endothelial cells for developing human vascular tissue models. It has many applications including its use in neural research involving oligodendrocyte precursor cells, Schwann cells, astrocytes, and microglia. It plays a role in bone formation, regulates mesenchymal stem cell differentiation, and serves as a survival factor for chondrocytes, hematopoietic stem cells, and tumor cells.Qkine recombinant human VEGF165 protein is a highly pure and potent 38 kDa homodimer, disulfide-linked consisting of two 165 amino acid polypeptide chains. It is an animal origin free (AOF) and carrier protein-free (CF) isoform of VEGF for high efficiency.
- Human TGF-β3 protein is a member of the Transforming Growth Factor family, a family involved in regulating cell survival, proliferation and differentiation.1 TGF-β3 is used in human pluripotent stem cell maintenance medias, such as B8 media.2 High purity and bioactivity for reproducible results. Human TGF-β3 protein dimer, animal-origin free (AOF) and carrier-protein free (CF).
- Sonic hedgehog protein (Shh) is a member of the Hedgehog family with an essential role in embryonic development, tissue regeneration, and tumorigenesis. Shh induces the cell fate and patterning of neural progenitors in ventral domains at various levels in the forebrain, midbrain, hindbrain, and spinal cord. It has many applications in the neural stem cell field where it plays a significant role in differentiating human-induced pluripotent stem cells (iPSC) towards motor neurons and interneurons. Also, it induces the patterning of organoids and embryos in culture.Recombinant Shh protein has a molecular weight of 19.8kDa. This protein is animal-free (AOF), carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Sonic hedgehog protein is suitable for the culture of reproducible and high-quality neurons and organoids.
- Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.
- Recombinant human r-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor. In epithelial tissues LGR5 marks the stem cell population. This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population. R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells. This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).
- R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signaling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.Recombinant human R-spondin 1 protein (RSPO1) protein is the bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1. Those are necessary and sufficient for Wnt signaling potentiation and are the essential domains for activity in stem cell and organoid culture.This protein has a molecular weight of 13 kDa and is animal-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk006 is suitable to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
- Mouse/rat noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.Qkine recombinant mouse noggin protein is a highly pure 46kDa bioactive dimer for reproducible results (animal-free and carrier-protein free).
- Human noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.Qkine human noggin protein is a highly pure 46kDa bioactive dimer for reproducible results in organoid culture.
- Macrophage colony-stimulating factor (M-CSF) is a cytokine that regulates the survival, proliferation, differentiation, and functional activation of monocytes, such as macrophages and dendritic cells. Various cells produce and release M-CSF, such as endothelial cells, fibroblasts, osteoblasts, smooth muscle, and macrophages. M-CSF binds to the CSF-1 receptor (CSF-1R) leading to the activation of the PI3K-AKT and AMPK signaling.M-CSF has been used in vitro for the differentiation of human induced pluripotent stem cell (iPSC)-derived macrophages as well as the maintenance and development of monocytes in hematopoietic stem cell culture. M-CSF has also been used to influence macrophage polarization towards an anti-inflammatory or M2 macrophage phenotype.A highly bioactive, animal-origin-free (AOF), carrier protein-free 36.8 kDa recombinant protein with exceptional lot-to-lot consistency.
- Human KGF protein (FGF-7) is a member of the FGF family secreted by mesenchymal cells and has a paracrine effect on neighbouring epithelial cells.1 KGF protein is used in the culture of lung, breast, liver, and bladder organoids.2 In vivo, KGF is involved in wound healing and development of kidney, thymus, and hippocampus.1High purity and bioactivity 19 kDa human FGF-7 / KGF protein, animal-origin free (AOF) and carrier-protein free (CF).
- Recombinant porcine HGF protein is a potent, high-purity NK1 isoform of porcine hepatocyte growth factor (HGF). Porcine Recombinant Protein HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding. Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk060 Bovine HGF (NK1). The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
- Neurotrophin 3 protein is part of the neurotrophin family and plays a crucial role in embryonic development and the maintenance and neuroprotection of the adult nervous system.NT-3 protein is used in cell culture to promote the differentiation and survival of specific neural subpopulations in both the central nervous system and peripheral nervous system such as sensory neurons, cortical neurons, and oligodendrocytes. It is also involved in the maintenance of endothelial cells and myocardial cells.Recombinant Neurotrophin 3 protein is a non-covalently linked homodimer with a molecular weight of 27.3 kDa.Qkine NT3 protein is animal-free (AOF), carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency. It is suitable for the culture of reproducible and high-quality cortical neurons and oligodendrocytes.
- Hepatocyte growth factor (HGF) is a multi-functional cytokine that promotes the growth and proliferation of various cell types including mesenchymal, endothelial, and epithelial cells. It is involved in embryonic development, cell motility, survival, angiogenesis, and tissue regeneration. In cell culture, HGF is used to promote the differentiation of mesenchymal cells towards hepatocytes, osteocytes, and endothelial cells. Recombinant human HFG NK1 protein is a potent naturally occurring isoform of HGF. This protein is animal-free, carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency with a molecular weight of 20 kDa. It promotes efficient differentiation of human induced pluripotent stem cells to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α.
- Recombinant bovine HGF protein is a potent, high-purity NK1 isoform of bovine hepatocyte growth factor (HGF). Bovine HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding. Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk061 Porcine HGF (NK1). The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
- Human/bovine/porcine Gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to Noggin for optimisation of intestinal organoid culture and iPSC differentiation.Qk015 has been optimized by our experts for exceptionally high-purity production in E.coli and bioactivity. 18 kDa high purity dimeric protein, animal-free and carrier protein free
- Epidermal growth factor (EGF), a member of the EGF family of proteins, plays a significant role in regulating cellular processes such as cell growth, proliferation, differentiation, development, and tissue homeostasis. It is an essential growth factor in many stem cells and organoid culture media.In cell culture, mouse EGF is crucial for stem cell maintenance, proliferation, differentiation, organoid culture growth and survival. It is also widely used for disease modelling, drug screening, tissue engineering, and regenerative medicine. Overall, EGF is a key player in cellular regulation and functions.Mouse EGF is a high purity protein with a molecular weight of 6.1 kDA. This protein is carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qkine mouse EGF is suitable for the culture of reproducible and high-quality stem cells and various tissue-specific organoids.
- Transforming Growth Factor beta 2 (TGF-β2), part of the TGF-β superfamily, regulates a wide array of cellular processes, including proliferation, differentiation, wound healing, apoptosis, metabolism, embryogenesis, and tissue repair. It is an essential growth factor in many embryonic and induced pluripotent stem cell culture media.In cell culture, TGF-beta 2 is crucial for directing cell fate, especially in stem cells and differentiation and facilitating wound healing processes. As a modulator of immune responses and a factor in tumour development, TGF-β2's involvement extends to immunomodulation and interaction within the tumour microenvironment. Its other applications in cell culture include stimulating protein expression and secretion. Overall, TGF-beta 2 emerges as a key player in cellular regulation with diverse biological functions.TGF-beta 2 is a high purity protein with a molecular weight of 25.4 kDa. This protein is carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qkine TGF-beta 2 is suitable for the culture of reproducible and high-quality stem cells and other relevant cells.
- Tumor necrosis factor-alpha (TNF-α) is a cytokine involved in cell signaling, primarily produced by immune cells like macrophages. It plays a central role in inflammation, immunity, and regulating cell survival and death. TNF-α binds to its receptors, TNF receptor 1 and TNF receptor 2, triggering pro-inflammatory responses, apoptosis, and immune cell activation.Human TNF-α has a molecular weight of 17.3 kDa. This protein is carrier-free and protein tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk083 TNF-alpha is suitable for the culture of reproducible and high-quality hematopoietic stem cells and other relevant cells.
- Interleukin-6 (IL-6) is a multifunctional cytokine that regulates immune responses and inflammation. It is produced by various cells, including immune cells such as T cells and macrophages, as well as non-immune cells like fibroblasts and endothelial cells. IL-6 plays a critical role in infection defense and tissue damage by promoting inflammation and activating immune cells. IL-6 is involved in various physiological processes, including the regulation of metabolism, tissue repair, and hematopoiesis. Human IL-6 has a molecular weight of 20.9 kDa. This protein is carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Interleukin-6 is suitable for the culture of reproducible and high-quality hematopoietic stem cells and other relevant cells.
- Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine that is secreted primarily by mast cells, T-cells, eosinophils, and basophils. IL-4 plays a crucial role in hematopoiesis, the regulation of antibody production, the stimulation of activated B cell and T cell proliferation, and the differentiation of B cells into plasma cellsIL-4 induces the expression of class II MHC molecules on resting B-cells and aids regulation of the low-affinity Fc receptor for IgE (CD23) expression on lymphocytes and monocytes. IL-4 additionally is a key regulator in adaptive immunity.Human IL-4 has a molecular weight of 15.1 kDa. This protein is carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. Qk092 is suitable for the culture of reproducible and high-quality stem cells and other relevant cells.
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