Recombinant human EGF protein (Qk011)

Product: Qk011 Category:

Price range: £60.00 through £190.00

Qk011 EGF vial image
Recombinant human EGF protein (Qk011) - Image 2
Recombinant human EGF protein (Qk011) - Image 3

Human epidermal growth factor (EGF) protein is a potent EGF-family growth factor used in many human and mouse organoid and stem cell culture systems including intestinal and tumor organoid culture. EGF is also used in epithelial cell culture.

Qkine human EGF (Qk011) is a highly pure, animal origin-free, bioactive 6.3 kDa recombinant protein, making it ideal for use in chemically defined induced pluripotent stem cell (iPSC), embryonic stem cell (ESC) and organoid culture media.

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Price range: £60.00 through £190.00

1000µg will be despatched as 2 x 500µg

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Summary:

  • High purity human EGF protein (residues 971-1023, Uniprot: P01133)
    •
  • 6.3 kDa

  • >98%, by SDS-PAGE quantitative densitometry

  • Expressed in E. coli

  • Animal origin-free (AOF) and carrier protein-free

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from acetonitrile, TFA

  • Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Handling and storage FAQ

Featured applications:

  • Tumor organoid culture

Frequently used together
Alternative proteins

EGF activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of EGF for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 11.6 pM (72.8 pg/mL). Data from Qk011 lot #10. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

EGF protein migrates as a single band at ~12 kDa in non-reducing (NR) and 9 kDa in reducing (R) conditions. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.  Data from Qk011 lot #011.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Recovery from stock vial:  >95%

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

MSDS file

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Qkine EGF is as biologically active as a comparable alternative supplier protein. Quantitative luciferase assay with Qkine EGF (Qk011, green) and alternative supplier EGF (Supplier B, black). Cells were treated in triplicate with a serial dilution of EGF for 3 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. EC50s 0.11 ng/ml.

Technote | EGF (Qk011) stability in mediaTechnote | EGF (Qk011) bioactivity 1Technote | EGF (Qk011) bioactivity 2Technote | porcine EGF (Qk064) vs human EGF (Qk011) bioactivity

Protein background

Members of the human epidermal growth factor family are synthesized as type I transmembrane precursor proteins, often containing several EGF domains in the extracellular region. Proteolysis yields mature proteins that are released from the cell surface [1]. Epidermal growth factor binds to high-affinity EGF receptors (EGFRs) and promotes receptor dimerization and clustering leading to activation of downstream signaling pathways, including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling [2].

Human epidermal growth factor (EGF) stimulates cell proliferation and differentiation. It is used extensively in induced-pluripotent stem cell (iPSC) and embryonic stem cell (ESC) culture systems for the successful expansion and differentiation of epithelial, neural, mesoderm and hematopoietic lineages [3]. EGF also stimulates the differentiation of mesenchymal stem cells (MSC) into bone-forming cells [4].

Recombinant EGF protein is a key component of many organoid media, along with other stem cell niche factors, R-Spondin 1, noggin or gremlin, Wnt3a and FGF-10. In addition, recombinant EGF is used for establishing and maintaining intestine, stomach, liver, pancreas, brain and cancer organoids [5].

Additional resources

Publications using Recombinant human EGF protein (Qk011)

FAQ

What is EGF?

Epidermal growth factor (EGF) is a growth factor which stimulates cell proliferation and differentiation.

Where is EGF found?

Originally found in salivary glands and urine, EGF is known to be expressed in many tissues, including platelets.

What does EGF do?

EGF primarily stimulates growth of epidermal and epithelial cells, but is also used for expansion of stem cells in feeder-free culture systems.

What does EGF bind to?

Epidermal growth factor receptor (EGFR) family are receptor tyrosine kinase (RTK) proteins. There are 4 members: EGFR (ErbB1, HER1), ErbB2 (HER2), ErbB3 (HER3) and ErbB4 (HER4).

What is the EGF signaling pathway?

Tyrosine phosphorylation of EGF receptors stimulates various signaling pathways, including Ras/MAPK, PLCγ1/PKC, PI3 kinase/Akt, and STAT pathways.

How is EGF used in cell culture?

EGF can be added to many stem cell cultures to maintain them in their pluripotent state.

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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