SB431542 (Qk1103) Price range: £110.00 through £190.00View product
SB431542 (Qk1103)
Price range: £110.00 through £190.00
SB431542 is a potent and selective inhibitor of ALK4, ALK5 and ALK7 receptors which activate the TGF-β/activin/nodal pathway. This pathway is essential for maintaining pluripotency in embryonic (ESC) and induced pluripotent stem cells (iPSC). In cell culture, SB431542 can be used for induced differentiation of ESC and iPSC into mesenchymal lineages, cardiac and neural differentiation.
Qkine SB431542 (Qk1103) is highly pure and validated in iPSC differentiation into ectoderm.
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Price range: £110.00 through £190.00
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Summary:
- Cas No. 301836-41-9
- Molecular Weight: 384.4
Storage: powder -20°C for 1 year, in solvent -80°C for 1 year
Solubility EtOH 3.85 mg/ml, DMSO 48.5 mg/ml
Featured applications:
Differentiation of iPSC into ectoderm
Neural differentiation from pluripotent stem cells
Differentiation of induced pluripotent stem cells to astrocytes
Differentiation of midbrain dopaminergic neurons
Frequently used together
- Recombinant human noggin protein (Qk034)
- Recombinant FGF2-G3 (145 aa) protein (Qk052)
- Recombinant human EGF protein (Qk011)
- Recombinant human CNTF protein (Qk063)
- HiDef® N-2 neural medium supplement
- Recombinant human BDNF protein (Qk050)
- Recombinant human GDNF protein (Qk051)
- Recombinant human TGF-β3 protein (Qk054)
- Recombinant human FGF-8a protein (Qk059)
- Recombinant human activin A protein (Qk001)
- Recombinant human BMP-4 protein (Qk038)
Further quality assays
>99% purity
HPLC/HNMR: Consistent with structure
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Preparation of SB431542 stock solution in DMSO/EtOH
| 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.60 ml | 13.01 ml | 26.02 ml |
| 5 mM | 0.52 ml | 2.60 ml | 5.20 ml |
| 10 mM | 0.26 ml | 1.30 ml | 2.60 ml |
Product background
SB431542 is a potent and selective inhibitor of TGF-β type I Receptors ALK5 (IC50 = 94 nM) and ALK4 (IC50 = 140 nM) and ALK7 [1,2]. SB431542 does not have an effect on BMP, ERK, JNK, or p38 MAP kinase pathways or signaling pathways activated in response to serum [2]. TGF−β superfamily ligands including activins and nodal signal through these receptors, mediating essential cellular processes related to development, would healing and immune regulation [2, 3].
In embryonic stem cells (ESCs) activin/nodal signaling in combination with FGF-2 maintains pluripotency through the ALK4/5/7 receptor pathway, addition of SB431542 reduces expression of pluripotency marker Oct-4 [4]. SB431542 can also be used to induce differentiation of both ESCs and induced pluripotent stem cells (iPSCs) into mesenchymal stem cells (MSCs) [5]. As part of cell culture media, SB431542 enhances differentiation into neurons [6, 7], astrocytes and cardiomyocytes [7].
FAQ
How do I prepare stock solutions of Qkine small molecules?
- Briefly centrifuge the vial to recover any compound from the cap.
- Add appropriate volume of recommended solvent to the vial to create the desired concentration stock solution (see product page for recommended solvent and volume).
- Vortex to ensure solvent has contacted the walls of the vial to recover all compound.
- To fully solubilize we recommend warming to 37˚C for 5 minutes and/or sonicating in short bursts (10 sec) for up to 5 minutes.
- Vortex thoroughly and prepare stock aliquots, store -20 or -80.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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