Y-27632 dihydrochloride (Qk1104) Price range: £75.00 through £100.00View product
Y-27632 dihydrochloride (Qk1104)
Price range: £75.00 through £100.00
Y-27632 dihydrochloride (Y-27632.2HCl) is an orally potent, ATP-competitive inhibitor of Rho associated coiled coil protein kinase (ROCK). ROCK pathway plays an important role in cell polarity, proliferation, adhesion and migration. Y-27632 dihydrochloride can be used in embryonic and pluripotent stem cell culture to decrease apoptosis and increase survival and proliferation, particularly during passaging and thawing.
Qkine Y-27632 dihydrochloride (Qk1104) is highly pure and validated in iPSC culture and differentiation.
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Price range: £75.00 through £100.00
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Summary:
- Cas No. 129830-38-2
- Molecular Weight: 320.26
Chemical name: 4-[(1R)-1-aminoethyl]-N-4-pyridinyl-trans-cyclohexanecarboxamide, dihydrochloride
Formula: C14H21N3O·2HCl
Storage: powder -20°C for 1 year, in solvent -80°C for 1 year
Solubility in DMSO 255 mg/ml, water 32 mg/ml
Featured applications:
Induced pluripotent stem cell culture and maintenance
Differentiation of iPSC into endoderm, mesoderm and ectoderm
Differentiation of midbrain dopaminergic neurons
Stem cell differentiation into neural and microglial lineages
Differentiation of induced pluripotent stem cells to astrocytes
Frequently used together
- HiDef® ITS cell culture supplement
- HiDef® N-2 neural medium supplement
- Recombinant human noggin protein (Qk034)
- Recombinant FGF2-G3 (145 aa) protein (Qk052)
- Recombinant human CNTF protein (Qk063)
- Recombinant human EGF protein (Qk011)
- Recombinant human activin A protein (Qk001)
- Recombinant human BDNF protein (Qk050)
- Recombinant human GDNF protein (Qk051)
Related discovery kits
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Further quality assays
>99% purity
HPLC/HNMR: Consistent with structure
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Preparation of Y-27632 dihydrochloride stock solution in DMSO/H2O
| 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.12 ml | 15.61 ml | 31.22 ml |
| 5 mM | 0.62 ml | 3.12 ml | 6.24 ml |
| 10 mM | 0.31 ml | 1.56 ml | 3.12 ml |
| 20 mM | 0.16 ml | 0.78 ml | 1.56 ml |
Product background
Y-27632 is a selective and potent ATP-competitive inhibitor of ROCK1 (Ki = 0.22 μM) and ROCK2 (Ki = 0.03 μM) [1]. ROCK/Rho-kinases are serine/threonine kinases which act as effectors for the small GTPase Rho, they play important roles in actomyosin cytoskeleton organization, regulating cell contraction, motility and polarization [2]. Inhibition of ROCK inhibits membrane blebbing and apoptosis caused by overactivation of myosin and the actin cytoskeleton [3].
Y-27632 dihydrochloride has been shown to increase the proliferation, migration and pluripotency in pluripotent stem cell types including embryonic stem cells [4, 5] and induced pluripotent stem cells [5]. It has also been shown to benefit many multipotent stem cells, for example, human periodontal ligament stem cells [6], murine prostate stem/progenitor cells [7]. In addition to its benefit in stem cell culture Y-27632 can be used to reduce apoptosis and mediate immortalization in primary cells such as keratinocytes [3] and epithelial cells [8].
Additional resources
- Weekend-free human induced pluripotent stem cell culture using thermostable FGF-2 (bFGF) and animal origin-free TGF-β1 and vitronectin for improved colony homogeneity (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into astrocytes (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into microglia (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into dopaminergic neurons (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into neuroectoderm (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into mesoderm (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into endoderm (PDF)
FAQ
How do I prepare stock solutions of Qkine small molecules?
- Briefly centrifuge the vial to recover any compound from the cap.
- Add appropriate volume of recommended solvent to the vial to create the desired concentration stock solution (see product page for recommended solvent and volume).
- Vortex to ensure solvent has contacted the walls of the vial to recover all compound.
- To fully solubilize we recommend warming to 37˚C for 5 minutes and/or sonicating in short bursts (10 sec) for up to 5 minutes.
- Vortex thoroughly and prepare stock aliquots, store -20 or -80.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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