Astrocyte differentiation kit (Qk520) Original price was: £815.00.£505.00Current price is: £505.00.
Astrocyte differentiation kit (Qk520)
Original price was: £815.00.£505.00Current price is: £505.00.
For the differentiation of induced pluripotent stem cells into astrocytes.
Astrocytes are the most abundant glial cells in the human central nervous system and are essential for maintaining healthy brain function. They regulate neurotransmitter uptake, ion and metabolic homeostasis, synaptic activity, and blood–brain barrier integrity, while also contributing to neuroimmune signaling, inflammation, injury response, and neuronal survival.
The kit includes carefully optimized growth factors required to efficiently guide iPSCs toward astrocytes.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Original price was: £815.00.£505.00Current price is: £505.00.
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Bioactivity Guaranteed
Manufactured in our Cambridge, UK laboratories
Lyophilized
- See reconstitution instructions for individual kit components
Featured applications:
Differentiation of induced pluripotent stem cells to astrocytes
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Immunocytochemistry of astrocyte markers in differentiated iPSCs. Phase contrast image (A); GFAP & Hoechst 33258 (green expression & blue expression, B); GFAP (green, C); Phase contrast image (D); ALD1L1 & Hoechst 33258 (green expression & blue expression, E); ALD1L1 (Green, F); Phase contrast image (G); EAAT1 & Hoechst 33258 (green expression & blue expression H); EAAT1 (green, I). Phase contrast image (J); EAAT2 & Hoechst 33258 (green expression & blue expression, K); EAAT2 (green, L). Scale bar = 150 µm. iPSC differentiated using the astrocyte differentiation kit (Qk520).
Application note | Differentiation of induced pluripotent stem cells (iPSCs) into astrocytes
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Dopaminergic neurons are specialized nerve cells responsible for the synthesis, storage, and release of the neurotransmitter dopamine. These neurons play a pivotal role in several critical brain functions, including motor control, reward, motivation, and regulation of mood [1]. Dopaminergic neurons are primarily located in the substantia nigra and the ventral tegmental area of the brain. They project to various brain regions, including the striatum, prefrontal cortex, and limbic system, forming complex networks that influence a variety of behaviors and physiological processes [2].
Dopaminergic neurons are crucial in motor control, as evidenced by their degeneration in Parkinson’s disease, a condition characterized by motor symptoms such as tremors, rigidity, and bradykinesia. In psychiatric disorders, dysregulation of dopaminergic signaling is implicated. For instance, hyperactivity of dopaminergic pathways is associated with the positive symptoms of schizophrenia, while diminished dopamine function is linked to anhedonia and lack of motivation seen in depression [2]. Differentiating iPSCs into dopaminergic neurons is an area of research with significant implications for treating neurological disorders, especially Parkinson’s disease.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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