Recombinant human noggin protein (Qk034)

Product: Qk034 Category: Stem cells

Price range: £230.00 through £2,900.00

Recombinant human noggin protein (Qk034) - Image 2

Human noggin is a bone morphogenetic protein (BMP) antagonist which regulates cell differentiation and growth. Noggin is used in the culture of intestinal, pancreatic, lung and tumor-derived organoids and the maintenance of undifferentiated embryonic stem cells (ESC) and for stem cell differentiation into neural and microglial lineages.

Qkine human noggin (Qk034) is a highly pure, animal origin-free, bioactive 46 kDa dimer for reproducible results in organoid culture.

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Price range: £230.00 through £2,900.00

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Summary:

High purity human noggin protein (Uniprot: Q13253)

46 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)

Handling and storage FAQ

Featured applications:

Tumor organoid culture
Epithelial organoid culture
Stem cell differentiation into neural and microglial lineages

Alternative protein names

Species reactivity

Frequently used together

Related discovery kits

Alternative proteins

Human noggin is a BMP inhibitor, and its activity is determined by inhibition of BMP-2 (Qk007) activity in a BMP-2 responsive firefly luciferase reporter assay. HEK293T cells were treated in triplicate with a serial dilution of noggin and a standard concentration of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 1.5 nM (70.1 ng/ml). Data from Qk034 lot #104291.

Noggin protein (Qk034) has an unusual migration in non-reduced (NR) SDS-PAGE due to the non-covalent dimer which is the active protein. Similar migration in SDS-PAGE is seen for gremlin-1, a related BMP antagonist. The identity of the purified dimeric protein was confirmed using mass spectrometry. Upon reduction, the protein monomer migrates at 23 kDa. Purified recombinant human noggin protein (7 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk034 lot #104285.

Further quality assays

Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)

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MSDS file

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Qkine human noggin is as biologically active as a comparable alternative supplier protein. Quantitative luciferase assay with Qkine human noggin (Qk034, green) and alternative supplier noggin (Supplier B, black) inhibition of BMP-2 (Qk007). Cells were treated in triplicate with a serial dilution of noggin and a standard concentration of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.

Technote | Noggin (Qk034) bioactivity

Protein background

Noggin is a 64 kDa homodimeric glycoprotein expressed during embryonic development and in adult cells [1]. Noggin is an extracellular antagonist of BMP cell signaling. BMPs are part of the larger TGFβ family and regulate embryonic, fetal and adult development in all vertebrates [1, 2]. Noggin binds to BMP-2, -4, -6 and -7, preventing interaction of the ligands with their receptor, and consequently blocking downstream activation of signaling cascades [2,3].

Through its control of BMP signaling, noggin plays a crucial role in embryonic patterning, as well as in the development of the neural tube, teeth, hair follicles, and the eye [4]. In cell cultures, noggin is used in the maintenance of pluripotent stem cells, differentiation of iPSCs to neural lineages, and in a large variety of organoid culture systems including intestine, liver and lung organoids [4, 5].

Background references

[1] Krause, C., Guzman, A. and Knaus, P. Noggin, Int. J. Biochem. Cell Biol., 43:4 (2011). doi.org/10.1016/j.biocel.2011.01.007.
[2] La Rosa, I., Camargo, L.S., Pereira, M.M. et al. Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos. Reprod Biol Endocrinol 9:18 (2011). doi.org/10.1186/1477-7827-9-18.
[3] Rifas, L. The Role of Noggin in Human Mesenchymal Stem Cell Differentiation. J. Cell. Biochem. 100:824–834 (2007). doi.org/10.1002/jcb.21132.
[4] Urbischek, M., Rannikmae, H., Foets, T. et al. Organoid culture media formulated with growth factors of defined cellular activity. Sci Rep 9, 6193 (2019). doi.org/10.1038/s41598-019-42604-0
[5] Wang,G., Zhang, H., Zhao, Y., Li, J., Cai, J., Wang, P., Meng, S., Feng, J., Miao, C., Ding, M., Li, D. and Deng, H. Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers. Biochem Biophys Res Commun 330:3 (2005). doi.org/10.1016/j.bbrc.2005.03.058.

Additional resources

Publications using Recombinant human noggin protein (Qk034)

Discovery and optimization of a guanylhydrazone-based small molecule to replace bFGF for cell culture applications

Feofanov M, Daubner GM, Saltalamacchia A et al.
DOI: https://doi.org/10.1101/2024.10.30.620903
Human epidermis organotypic cultures, a reproducible system recapitulating the epidermis in vitro

Agarwal R, Dittmar T, Beer HD et al.
DOI: doi: 10.1111/exd.14823
Identification of molecular and functional subtypes using chronic pancreatitis patient-derived organoid models

Osorio-Vasquez V, Lumibao JC, Peck KL et al.
DOI: https://doi.org/10.1101/2024.10.30.620903
Spatial profiling of early primate gastrulation in utero

Bergmann S, Penfold CA, Slatery E et al.
DOI: doi: 10.1038/s41586-022-04953-1
The effect of extracellular matrix on the precision medicine utility of pancreatic cancer patient-derived organoids

Lumibao JC, Okhovat SR, Peck KL et al.
DOI: doi: 10.1172/jci.insight.172419

FAQ

What is noggin?

Noggin is a bone morphogenic protein (BMP) inhibitor.

Is noggin a cytokine or growth factor?

Neither, noggin is a BMP agonist.

What does noggin do?

Through binding to BMP and stopping BMP signaling, noggin controls the development of many embryonic structures.

What does noggin bind to?

Noggin binds to extracellular BMPs, stopping them binding to their receptor.

How is noggin used in cell cultures?

Noggin can be added to embryonic and induced pluripotent stem cell cultures to preserve pluripotency.

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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Recombinant human noggin protein (Qk034)

Summary:

Featured applications:

Further quality assays

Protein background

Additional resources

Publications using Recombinant human noggin protein (Qk034)

Discovery and optimization of a guanylhydrazone-based small molecule to replace bFGF for cell culture applications

Human epidermis organotypic cultures, a reproducible system recapitulating the epidermis in vitro

Identification of molecular and functional subtypes using chronic pancreatitis patient-derived organoid models

Spatial profiling of early primate gastrulation in utero

The effect of extracellular matrix on the precision medicine utility of pancreatic cancer patient-derived organoids

FAQ

Receive an Amazon gift voucher when you leave us a review.

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