Recombinant human BMP-4 protein (Qk038)£205.00 – £2,900.00View product
Recombinant human BMP-4 protein (Qk038)
£205.00 – £2,900.00
Human BMP-4 (bone morphogenetic protein 4) is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP-4 protein plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.
Qkine BMP-4 is a highly pure, animal origin-free and carrier protein-free 24 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP-4 protein.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£205.00 – £2,900.00
1000µg will be despatched as 2 x 500µg
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Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity optimized human BMP-4 protein (Uniprot: P12644)
- 24 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Induced pluripotent and embryonic stem cell differentiation and maintenance
Chemically defined media optimization
Frequently used together
- Recombinant human activin A protein (Qk001)
- Recombinant mouse LIF protein (Qk018)
- Recombinant human LIF protein (Qk036)
- Recombinant human EGF protein (Qk011)
- Recombinant human FGF-2 (145 aa) protein (Qk025)
- Recombinant human VEGF 165 protein (Qk048)
- Recombinant human FGF-18 protein (Qk069)
- Recombinant human GDF-5 protein (Qk070)
- Recombinant human FGF-4 protein (Qk004)
- Recombinant human TGF-β2 protein (Qk072)
Alternative proteins
BMP-4 activity was determined using the BMP4-responsive firefly luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 30.2 pM (0.72 ng/ml). Data from Qk038 lot #104294.
BMP-4 protein (Qk038) dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form). Purified recombinant protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk038 lot #104294.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine BMP-4 has higher bioactivity than bacterial and mammalian-expressed BMP-4 from alternative suppliers. Quantitative luciferase assay with Qkine BMP-4 (Qk038, green) and BMP-4 from alternative suppliers (Supplier A, blue; Supplier B, black). Cells were treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Technote | BMP-4 (Qk038) bioactivityTechnote | BMP-4 (Qk038) lot-to-lot bioactivityProtein background
Bone morphogenetic protein 4 (BMP-4) is part of the TGF-β superfamily of growth factors. BMP-4 is a key regulator of embryogenesis with roles in gastrulation, establishment of a dorsal-ventral axis, and regulation of limb development [1]. As their name suggest the BMP family is involved in the development and maintenance of bone and cartilage; in adults BMP-4 has roles bone and cartilage metabolism, as well as neurogenesis [2] and hematopoietic stem cell homing [3]. BMP-4 protein acts through two serine-threonine kinase receptors; Bone morphogenetic protein receptor (BMPR) 1 and BMPR2, via these receptors BMP-4 invokes Smad and map kinase pathways and consequently alters target gene transcription [1].
Publications using Recombinant human BMP-4 protein (Qk038)
-
High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humans
Stucchi S, Sepulveda-Rincon LP, Dion C et al.
DOI: https://doi.org/10.1101/2025.01.14.632914 -
Limited oxygen in standard cell culture alters metabolism and function of differentiated cells
Tan J, Virtue S, Norris DM et al.
DOI: doi: 10.1038/s44318-024-00084-7 -
Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac progenitor Specification during Mouse Gastrulation
Drozd AM, Mariani L, Guo X, Goitea V, Menezes NA and Ferretti E.
DOI: doi: 10.3390/ijms231810307 -
Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering
Truszkowski L, Bottini S, Bianchi SÂ et al.Â
DOI: https://doi.org/10.12688/openreseurope.18245.1 -
Single Cell Transcriptional Perturbome in Pluripotent Stem Cell Models
Balmas E, Ratto ML, Snijders KE et al.
DOI: http://dx.doi.org/10.2139/ssrn.4854180 -
STAT3 signalling enhances tissue expansion during postimplantation mouse development
Azami T, Theeuwes B, Ton M-LN et al.
DOI: https://doi.org/10.1101/2024.10.11.617785 -
OTX2 controls chromatin accessibility to direct somatic versus germline differentiation
Barbieri E and Chambers I
DOI: doi.org/10.1101/2025.02.18.638827
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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