Recombinant human BMP-4 protein (Qk038) Price range: £230.00 through £2,900.00View product
Recombinant human BMP-4 protein (Qk038)
Price range: £230.00 through £2,900.00
Human BMP-4 (bone morphogenetic protein 4) is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP-4 protein plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.
Qkine BMP-4 is a highly pure, animal origin-free and carrier protein-free 24 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP-4 protein.
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Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £230.00 through £2,900.00
500ug shipped as 5 x 100ug | 1000ug shipped as 10 x 100ug
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity optimized human BMP-4 protein (Uniprot: P12644)
- 24 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Induced pluripotent and embryonic stem cell differentiation and maintenance
Chemically defined media optimization
Frequently used together
- Recombinant human activin A protein (Qk001)
- Recombinant mouse LIF protein (Qk018)
- Recombinant human LIF protein (Qk036)
- Recombinant human EGF protein (Qk011)
- Recombinant human FGF-2 (145 aa) protein (Qk025)
- Recombinant human VEGF 165 protein (Qk048)
- Recombinant human FGF-18 protein (Qk069)
- Recombinant human GDF-5 protein (Qk070)
- Recombinant human FGF-4 protein (Qk004)
- Recombinant human TGF-β2 protein (Qk072)
- Recombinant human IL-34 PLUSâ„¢ protein (Qk091)
Alternative proteins
BMP-4 activity was determined using the BMP4-responsive firefly luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 30.2 pM (0.72 ng/ml). Data from Qk038 lot #104294.
BMP-4 protein (Qk038) dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form). Purified recombinant protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk038 lot #104294.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine BMP-4 has higher bioactivity than bacterial and mammalian-expressed BMP-4 from alternative suppliers. Quantitative luciferase assay with Qkine BMP-4 (Qk038, green) and BMP-4 from alternative suppliers (Supplier A, blue; Supplier B, black). Cells were treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Technote | BMP-4 (Qk038) bioactivityTechnote | BMP-4 (Qk038) lot-to-lot bioactivityTechnote | BMP-4 (Qk038) stabilityProtein background
Bone morphogenetic protein 4 (BMP-4) is part of the TGF-β superfamily of growth factors. BMP-4 is a key regulator of embryogenesis with roles in gastrulation, establishment of a dorsal-ventral axis, and regulation of limb development [1]. As their name suggest the BMP family is involved in the development and maintenance of bone and cartilage; in adults BMP-4 has roles bone and cartilage metabolism, as well as neurogenesis [2] and hematopoietic stem cell homing [3]. BMP-4 protein acts through two serine-threonine kinase receptors; Bone morphogenetic protein receptor (BMPR) 1 and BMPR2, via these receptors BMP-4 invokes Smad and map kinase pathways and consequently alters target gene transcription [1].
Additional resources
- Technote | BMP-4 (Qk038) bioactivity
- Technote | BMP-4 (Qk038) lot-to-lot bioactivity
- Technote | BMP-4 (Qk038) stability
- Stability of Qkine recombinant growth factors and cytokines in conditioned media (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into mesoderm (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into endoderm (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into microglia
- Poster: Pluripotent stem-cell derived organoids
- Brochure: Growth factors for neural and glial cell differentiation
- Brochure: Growth factors for enhanced organoid culture protocols
Publications using Recombinant human BMP-4 protein (Qk038)
-
High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humans
Stucchi S, Sepulveda-Rincon LP, Dion C et al.
DOI: https://doi.org/10.1101/2025.01.14.632914 -
Limited oxygen in standard cell culture alters metabolism and function of differentiated cells
Tan J, Virtue S, Norris DM et al.
DOI: doi: 10.1038/s44318-024-00084-7 -
Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac progenitor Specification during Mouse Gastrulation
Drozd AM, Mariani L, Guo X, Goitea V, Menezes NA and Ferretti E.
DOI: doi: 10.3390/ijms231810307 -
Refined and benchmarked homemade media for cost-effective, weekend-free human pluripotent stem cell culture
Truszkowski L, Bottini S, Bianchi SÂ et al.Â
DOI: doi.org/10.12688/openreseurope.18245.2 -
Single Cell Transcriptional Perturbome in Pluripotent Stem Cell Models
Balmas E, Ratto ML, Snijders KE et al.
DOI: http://dx.doi.org/10.2139/ssrn.4854180 -
STAT3 signalling enhances tissue expansion during postimplantation mouse development
Azami T, Theeuwes B, Ton M-LN et al.
DOI: https://doi.org/10.1101/2024.10.11.617785 -
OTX2 controls chromatin accessibility to direct somatic versus germline differentiation
Barbieri E and Chambers I
DOI: doi.org/10.1101/2025.02.18.638827
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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