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Recombinant human BMP-4 protein (Qk038)
Human BMP-4 (bone morphogenetic protein 4) is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP-4 protein plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.
Qkine BMP-4 is a highly pure, animal origin-free and carrier protein-free 24 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP-4 protein.
Our robust animal origin-free manufacturing platform, along with rigorous quality control procedures, ensures exceptional bioactivity and consistent performance from lot to lot, guaranteeing outstanding performance in your applications.
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
500µg will be despatched as 5 x 100µg
1000µg will be despatched as 10x 100µg
Fast and free shipping.
Buy online with secure credit card or purchase order.
Summary
High purity optimised human BMP-4 protein (Uniprot: P12644)
>98%, by SDS-PAGE quantitative densitometry
24 kDa (dimer)
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10 mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Featured applications
iPSC and ESC maintenance and expansion
Chemically defined media
human
Species similarity:
bovine – 99%
porcine – 99%
mouse – 98%
rat – 98%
Bioactivity
BMP-4 activity is determined using the BMP4-responsive firefly luciferase reporter assay in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 30.2 pM (0.72 ng/mL). Data from Qk038 lot #104294.
Purity
BMP-4 protein (Qk038) dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form). Purified recombinant protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk038 lot #104294.
Further quality assays
Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine BMP-4 has higher bioactivity than bacterial and mammalian-expressed BMP-4 from alternative suppliers
Quantitative luciferase assay with Qkine BMP-4 (Qk038, green) and BMP-4 from alternative suppliers (Supplier A, blue; Supplier B, black). Cells were treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Qkine BMP-4 protein produces consistently high bioactivity lot-to-lot
Bioactivity was determined using a BMP-4-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of four independent lots of BMP-4 for 6 hours in triplicate.
Protein background
Bone morphogenetic protein 4 (BMP-4) is part of the TGF-β superfamily of growth factors. BMP-4 is a key regulator of embryogenesis with roles in gastrulation, establishment of a dorsal-ventral axis, and regulation of limb development [1]. As their name suggest the BMP family is involved in the development and maintenance of bone and cartilage; in adults BMP-4 has roles bone and cartilage metabolism, as well as neurogenesis [2] and hematopoietic stem cell homing [3]. BMP-4 protein acts through two serine-threonine kinase receptors; Bone morphogenetic protein receptor (BMPR) 1 and BMPR2, via these receptors BMP-4 invokes Smad and map kinase pathways and consequently alters target gene transcription [1].
Additional resources featuring recombinant human BMP-4 protein (Qk038)
Publications using recombinant human BMP-4 protein (Qk038)
STAT3 signalling enhances tissue expansion during postimplantation mouse development
bioRxiv preprint (2024) by Azami, T. et al.
Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac Progenitor Specification during Mouse Gastrulation
In International Journal of Molecular Science on 7 September 2022 by Drozd, A. et al.
Limited oxygen availability in standard cell culture alters metabolism and function in terminally-differentiated cells.
In The Embo Journal on 5 April 2024 by Tan, J., Virtue, S., et al.
Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering.
In Open Research Europe 2024 by Truszkowski, L., Bottini, S., Bianchi, S. et al.
Qkine products are intended solely for research purposes and ex vivo cell manufacturing. Not for therapeutic or diagnostic use.
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BMP4
Anonymous –
A BMP-4 that is less than half the price of our previous supplier and has at least equal activity. All good as far as we are concerned.
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