Technote | BMP-4 (Qk038)

Qkine BMP-4 has higher bioactivity than microbial, and mammalian-expressed BMP-4 from alternative suppliers.


Human bone morphogenetic protein 4 (BMP-4) is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells.

In addition, BMP-4 plays a role in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.

Bone morphogenetic protein 4 has a complex biochemical structure. Special care and expertise is required to manufacture BMP-4 at high purity and with good lot consistency. Ensuring high bioactivity allows lower concentrations of growth factors to be used in media for cost-effective cell culture.


Bioactivity was determined using a BMP-4-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of BMP-4 for 6 hours in triplicate. Firefly luciferase activity was measured and normalized to the control, Renilla luciferase.


Bioactivity comparison between leading suppliers shows Qk038 animal-free BMP-4 (expressed in E. coli) has a higher bioactivity than microbial and mammalian expressed BMP-4 from alternative suppliers.

Qkine BMP-4 bioactivity graph comparison

Qkine BMP-4 has higher bioactivity than alternative suppliers. Bioactivity of recombinant BMP-4 protein was determined by a luciferase assay. Qkine E. coli expressed BMP-4 (Qk038, green) has a higher bioactivity (EC50=19.4 pM) than alternative mammalian expressed (Supplier A, blue, EC50=133.5 pM) and E. coli expressed (Supplier B, grey, EC50=43.9 pM) BMP-4.

All our recombinant proteins are animal-free
& come with Bioactivity. Guaranteed.

Featured resources

Nine-point Quality Commitment

Qkine quality commitment

Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.

Quality policy

Qkine ISO 9001: 2015 certification

Learn more about our quality management system implemented to comply with the requirements of ISO 9001: 2015.


Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an
ISO 9001: 2015 certified facility.

Setting the standard in bioactive protein manufacturing

Our science team is here to help, please contact us if you have any questions.

Contact our expert team

Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.