LY294002 (Qk1102)
Price range: £75.00 through £100.00
LY294002 is a broad-spectrum inhibitor of phosphoinositide 3-kinases (PI3Ks). PI3K is a major regulator of cell proliferation, survival, motility and metabolism. LY294002 can be using in stem cell media to reduce proliferation and self-renewal, promoting differentiation.
Qkine LY294002 (Qk1102) is highly pure and validated in iPSC differentiation into endoderm and mesoderm lineages.
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Price range: £75.00 through £100.00
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Summary:
- Cas No. 154447-36-6
- Molecular Weight: 307.3
Formula: C19H17NO3
Storage: powder -20°C for 1 year, in solvent -80°C for 1 year
Solubility EtOH 10 mg/ml, DMSO 61.8 mg/ml
Featured applications:
Differentiation of iPSC into endoderm
iPSC-derived mesoderm differentiation
Neural differentiation from pluripotent stem cells
Induction of cardiomyocytes from iPSCs
Cancer research
Further quality assays
>99% purity
HPLC/HNMR: Consistent with structure
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Preparation of LY294002 stock solution in DMSO/EtOH
| 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.25 ml | 16.27 ml | 32.54 ml |
| 5 mM | 0.65 ml | 3.25 ml | 6.51 ml |
| 10 mM | 0.33 ml | 1.62 ml | 3.25 ml |
| 50 mM | 0.05 ml | 0.33 ml | 0.65 ml |
Product background
LY294002 is a broad-spectrum inhibitor of phosphoinositide 3-kinases (PI3Ks), inhibiting PI3Kα, PI3Kδ, and PI3Kβ (IC50=0.5/0.57/0.97 μM). LY294002 is also an inhibitor of DNA-PK (IC50=1.4 μM) and CK2 (IC50=98 nM) but not PI4K or MAP kinases [1]. PI3K is activated through growth factor stimulation leading to an increase into the secondary messenger phosphatidylinositol (3,4,5)-trisphosphate (PIP3) which recruits pleckstrin homology (PH) domain containing proteins. Through PIP3, PI3Ks activate major cellular signaling pathways such as Akt, GSK3α/β, mTORC1 and PDK1 [2, 3] and cross talk with the WNT/β-catenin, NF-κB and TGF-β signaling pathways [3]. Through activation of multiple downstream signaling pathways PIP3 stimulates cellular proliferation, survival, motility and metabolism and other processes considered oncogenic [2, 3].
PI3K signaling is important in the regulation of cancer stemness and the maintenance of undifferentiated embryonic stem cells (ESCs) and reprogramming of induced pluripotent stem cells (iPSCs) [3, 4]. In mesenchymal stem cells (MSCs), PI3K plays a role in osteogenic and adipogenic differentiation in combination with PDGF and TGF-β signaling pathways [4]. Inhibition of PI3K with LY294002 inhibits MSC proliferation and promotes neural differentiation, it can also be used to promote differentiation of human endometrial stem cells (hEnSCs) into motor neuron-like cells [5]. LY294002 can be used in ESC to inhibit the IGF/CK2 pathway to promote cardiomyocyte differentiation [6].
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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