FRS™ Pioneer – Fetal bovine serum replacement
Price range: £140.00 through £985.00
The first member of our FRS family, FRS™ Pioneer is a chemically defined, animal origin free alternative to fetal bovine serum (FBS) for broad-spectrum cell culture.
FRS™ Pioneer can be added to cell culture media in place of FBS to culture or cryopreserve cells without the use of any animal components or serum (i.e. 100 ml of FRS™ Pioneer is equivalent to 100 ml of FBS). This allows for reproducible cell culture and provides a cleaner background. For primary cells, retain 1% FBS or supplement with additional growth factors to achieve full serum replacement (app notes below).
To date, analyses indicate that intracellular signaling and cellular function are maintained between FBS and FRS™ Pioneer-cultured cells.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £140.00 through £985.00
Buy online with secure credit card or purchase order. For any questions, please email [email protected]
Summary:
Broad spectrum
Animal origin free (AOF)
Human specific product
Cost effective
Store at -20˚C, protect from light
- Product supplied in liquid form
Featured applications:
Primary cell culture
Culture of immortalized cell lines
Cryopreservation
Cell growth in microcarrier systems and on scaffolds
Exosome research and manufacturing
Chemically defined media
Cumulative population doublings over time across four primary cell types under fully defined serum-free conditions. GFs: AD-MSCs: 10 ng/ml PDGF-BB (Qk044) + 10 ng/ml FGF-2 (Qk025); Chondrocytes: 10 ng/ml FGF-2 + 1 ng/ml TGF-β1 (Qk010); Fibroblasts: 10 ng/ml FGF-2 + 10 ng/ml PDGF-BB; MuSCs: 2 ng/ml TGF-β1 + 50 ng/ml FGF-2. Note the muscle stem cells were grown with 20% supplementation (FBS or FRS Pioneer). Dashed vertical lines indicate passage events. Points indicate measured values; lines represent interpolated growth between passage events. Basal media alone failed to support proliferation of any cell type beyond 72 h.
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Product background
FRS™ Pioneer features:
- Drop in – an easy substitute for FBS in your laboratory processes
- Broad spectrum – grows a wide range of immortalized cell lines
- Chemically defined – ensuring batch consistency and research reproducibility, giving scientists greater experimental control and allowing for background-free, clean interpretation of signaling and functional data.
- Contaminant free – defined inputs align with regulatory demands and simplify downstream planning for clinical settings
- Cost-effective – priced on par with Premium FBS
- Animal origin free – an ethical, sustainable solution free of animal-derived components and associated supply volatility.
Read the quick start guide to get going.
FRS Pioneer has successfully been used by external scientists to achieve:
- Routine expansion of a broad range of immortalized cell lines (HEK, CHO, JURKAT, MCF7, A549, and many more)
- Cryopreservation when mixed with 10% DMSO
- Cleaner assays (e.g. reduced cytokine background)
- Cell growth in microcarrier systems and on scaffolds
- Transfection of cell lines for recombinant protein production
- Exosome research and manufacturing – no extracellular vesicle depletion necessary
- Reduction of FBS usage to 1% in nutritionally demanding primary and organoid systems
- Optimized, serum-free media for primary cells, by adding additional cell-specific needs to FRS Pioneer
And many more applications. Please reach out directly if there is a specific application or use case you’re interested in.
Adhesion and cell attachment
For adherent cells, attachment is handled modularly via surface choice or coatings. This allows users to select adhesion strategies appropriate to their system. The following strategies have been effectively used with FRS Pioneer to facilitate attachment:
- Coating cell culture plates with serum-containing media
- Cell-specific coatings such as vitronectin, fibronectin, laminin, collagen, gelatin, or Matrigel
- Coating with GECKO™, a broad-spectrum, animal-free and chemically defined coating developed by Media City Scientific
- Coating-free workflows in which adherence proteins are added directly to the media (please contact us if this is of interest).
Coating choice can influence attachment efficiency and achievable cell density.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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