GECKO™ – adherence solution£165.00
GECKO™ – adherence solution
£165.00
GECKO™ is a chemically defined, animal component free mix of adhesion factors to support adherent cell culture. Fetal bovine serum (FBS) contains a complex mixture of adhesion-promoting factors that enable attachment on standard tissue culture plastic. When culturing cells in serum-free or chemically defined media, these adhesion cues are often no longer present. As a result, many adherent cell types require an explicit surface strategy to support attachment, spreading, and early survival.
GECKO™ has been validated as a defined, animal-free coating which supports a broad range of adherent cell types.
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£165.00
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Summary:
- 1.25 mg GECKO™ adhesion factor cocktail
Animal origin free (AOF)
Store frozen, -5°C to -20°C
- Resuspend GECKO protein in PBS without calcium or magnesium at 12.5 µg/ml. Sterilize by 0.22 µm filtration. Store at −20°C in aliquots to minimize freeze-thaw cycles.
Featured applications:
Primary cell culture
Culture of immortalized cell lines
Chemically defined media
Serum-free media development
Cell attachment
Frequently used together
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Product background
Many cell types require the use of an adherence coating when culturing cells with FRS™ Pioneer—or any serum-free media—on standard TC plasticware. GECKO™ is a chemically defined and animal component free mix of lyophilized adhesion proteins that facilitate attachment of many adherent cell types to standard cell culture plates. GECKO™ supports serum-free adherence of most cell lines traditionally cultured in FBS without a coating. A minority of cell lines will perform optimally with a recombinant vitronectin coating (see below).
GECKO™ was developed to pair with FRS™ Pioneer as part of a fully chemically defined culture workflow.
Coating workflow for culturing adherent cells with FRS™ Pioneer
Using GECKO™ with FRS™ Pioneer allows:
- Separation of growth support (media) from adhesion (surface)
- Greater experimental control
- Reduced background from undefined serum components
GECKO™ can also be used with other serum-free or reduced-serum media systems.
When to use GECKO™
GECKO™ is a good starting point if you:
- Are transitioning from serum-containing media to serum-free or chemically defined media
- Want a single, animal-free coating compatible with many adherent cell lines
- Prefer a reproducible, defined alternative to serum-based pre-coating
- Are culturing adherent cells with FRS™ Pioneer and require attachment support
GECKO™ Mix (with fibronectin) has been validated with:
- Non-cancer epithelial (CHO, HEK)
- Low TGFβ cancer (MCF7)
- Mesenchymal (NIH-3T3)
- Glioblastoma (U87, A172)
- Renal carcinoma (ACHN)
- Muscle (C2C12)
- Skin (B16-F10)
When to use an alternative coating
You may require a different coating strategy if:
- Your cell line falls into a category previously validated with FRS™ Pioneer for vitronectin coating (see below).
- When culturing your cells in FBS-containing media, you typically use a coating (e.g. collagen, laminin).
- You observe poor attachment, spreading, or survival with GECKO™
In these cases, coatings including but not limited to vitronectin, laminin, fibronectin, collagen, Matrigel or gelatin may be more appropriate.
Vitronectin alone with FRS™ Pioneer has been validated with:
- High TGFβ cancer (A549, H460, SW480, DU145)
- Hepatocyte (Hep2G)
- Normal kidney (MDCK)
- Adenocarcinoma (HT-29)
Additional resources
- Animal-free, defined culture system preserving chondrogenic phenotype: FRS™ Pioneer and animal origin-free TGF-β1 (PDF)
- FRS™ Pioneer: A broad-spectrum, chemically defined and animal-free serum replacement for primary cell culture (PDF)
- Animal-free, defined media outperforms FBS for canine AD-MSC expansion: A case for lot-to-lot consistency (PDF)
- FRS™ Pioneer: Immortalized cells (PDF)
- GECKO™ - adherence solution user guide
- Coating Workflow for Culturing Adherent Cells with FRS™ Pioneer
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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