FRS™ Pioneer: Cryoprotection
Media City Scientific
Media City Scientific’s Fetal Bovine Serum Replacement Solution (FRS™) Pioneer is a drop-in chemically defined and animal component free FBS replacement. FRS™ Pioneer supports the growth of a broad range of cell lines but can also be substituted for FBS for cell cryopreservation. Mix 10% DMSO with 90% FRS™ Pioneer for broad-spectrum cryopreservation efficacy in the liquid or vapor phase of nitrogen.
FRS™ Pioneer is a drop-in, chemically defined FBS replacement that is:
> Cost effective – priced on par with premium FBS
> Consistent – enabling scientific reproducibility and reliability
> Animal-free – contains no animal derived components
> Effective – supports a broad range of cells at a similar level of performance to FBS
FRS™ Pioneer versus FBS for cell cryopreservation
Cells were frozen using standard methods in freeze media containing 10% DMSO with either 90% FBS or 90% FRS™ Pioneer. Cells were stored in a liquid or vapor phase nitrogen tank for a period of 1 week to 6 months. Cells were thawed using standard methods. Viability, proliferation, and function were analyzed. Data indicate that, similar to FBS, FRS™ Pioneer is an effective cryopreservation agent when mixed with 10% DMSO. Data sets were collected internally and externally.
Immortalized Cell lines
Tested: A549, CHO, MCF7
Growth of A549 cells following cryopreservation in FBS (red) or FRS™ Pioneer (green) -containing freeze media (3 months, LN2)
Primary Cells
Tested: Muscle stem cells, chondrocytes, fibroblasts
Viability of primary fibroblasts following cryopreservation in FBS (red) or FRS™ Pioneer (green) -containing freeze media (6 months, LN2)
External Case Study: PBMCs cryopreserved in vapor-phase N2 for 5-14 days using FRS™ Pioneer-containing freeze media retain viability and effector functions following thaw. Long-term biobanking study is in progress. Reach out for more details.
Further information
Media City Scientific
Media City Scientific manufactures FRS™ Pioneer, a fully chemically defined, animal-origin-free serum replacement engineered for broad primary cell type compatibility and lot-to-lot consistency. FRS™ Pioneer is available directly and through Qkine.
Qkine
Qkine are committed to raising the standards of growth factors, cytokines and related proteins to better support long-term and complex stem cell culture.
Enquiries: [email protected] | [email protected]
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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