As Noggin is a BMP inhibitor, the bioactivity of Qk033 Noggin and Supplier B Noggin is determined by inhibiting BMP-2 (Qk007) activity in a BMP-2 responsive firefly luciferase reporter assay. HEK293T cells are treated (in triplicate) with a serial dilution of Noggin and a standard concentration of BMP-2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.
The bioactivity comparison demonstrates that Qkine Noggin is more bioactive than Noggin from a major alternative supplier in our reporter assay. Qkine Noggin is highly pure, potent, and reliable for reproducible cultures of mouse embryonic stem cells.
Qkine Noggin and all Qkine recombinant proteins come with a Bioactivity Guarantee, guaranteeing our proteins to be reproducibly bioactive in your cultures.