For rapid testing of FGF-2 with thermostable FGF-2 protein, to determine whether the short half-life of FGF-2 is impacting cell fate.
FGF-2 is commonly used in stem cell culture for induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) maintenance and induced pluripotent and mesenchymal stem cell proliferation and differentiation.
FGF-2 has a bioactive half-life of less than 10 hours. Even with daily media changes, the level of FGF2 signaling in media fluctuates substantially. FGF2-G3 is a thermostable form of FGF2 with a bioactive half-life of over 7 days.
FGF-2 is typically available in two lengths – 145aa or 154aa. Researchers’ rationale for using one form over the other is often based on prior use rather than definitive data.
The thermostable FGF-2 Discovery Kit contains 4 different forms of FGF-2 to determine whether there is a biological impact of using one FGF-2 length over the other in both the wild type and thermostable forms, and to determine whether consistent levels of FGF2 signaling allows for more homogeneous culture.