Human BMP2 Qk007 protein purity SDS-PAGE lot #010

Recombinant human BMP-2 protein (Qk007)

Name: Recombinant human BMP-2 protein (Qk007)
SKU: Qk007
Availability: InStock

Human recombinant BMP-2 protein (bone morphogenetic protein 2) protein is member of the TGFβ family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.

26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of human BMP-2 protein, animal origin-free (AOF) and carrier protein-free.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1000µg will be despatched as 2 x 500µg

Fast and free shipping.
Buy online with secure credit card or purchase order.
For any questions, please email orders@qkine.com

Quote request

Bulk request

Description

Summary

High purity human bone morphogenetic protein 2, BMP-2 (Uniprot: P12643).
>98%, by SDS-PAGE quantitative densitometry
26 kDa (dimer)
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10 mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Handling and storage FAQ

Featured applications

Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes

Alternative protein names

Bone morphogenetic protein 2,BDA2, SSFSC, BMP-2A, BMP-2, Bone morphogenetic protein 2A

Species reactivity

human

species similarity:
mouse – 100%
rat – 100%
porcine – 100%
bovine – 100%

Frequently used together

Recombinant human activin A protein (Qk001)
Recombinant human FGF-2 (145 aa) protein (Qk025)

Bioactivity

BMP-2 activity is determined using the BMP-2 responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of BMP-2 for 6 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 0.3 nM (7.8 ng/mL). Data from Qk007 lot #10.

Purity

BMP-2 migrates as a single band at 26 kDa in non-reducing (NR) conditions and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk007 lot #010

Further quality assays

Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%

Request CoA/animal-free certificate

Download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Qkine BMP-2 is more biologically active than a comparable alternative supplier protein

Qkine BMP-2 has higher bioactivity than an alternative supplier BMP-2

Bioactivity was determined using a BMP-2-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of Qkine BMP-2 (Qk007, green) or alternative BMP-2 (supplier B, black). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk007 lot #204510.

View technote

Protein background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily. It plays numerous roles in the developing embryo, such as embryonic patterning along the dorso-ventral axis, organogenesis, limb bud formation, and bone and cartilage growth. BMP-2 protein is a potent differentiation factor and directs human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes [1] . Recombinant BMP-2 protein induces bone and cartilage formation in vitro and chondrogenesis in human adult mesenchymal stem cells [2].

Human bone morphogenetic protein 2 (BMP-2) is synthesized as a preproprotein consisting of N-terminal signal peptide, 259 amino acid residue pro-domain and 114 residue mature domain. Proteolytic removal of the propeptide enables mature BMP-2 protein to form active disulfide-linked homodimers, and heterodimers with BMP7.

Mature human BMP-2 protein shares 100% amino acid sequence identity with mouse and rat BMP-2. It also shares 85% amino acid sequence identity with the related protein, BMP-4, and less than 51% identity with other BMPs.

Background references

[1] Pera, M. F. et al. Regulation of human embryonic stem cell differentiation by BMP-2 and its antagonist noggin. J. Cell Sci. 117, 1269–80 (2004). doi.org/10.1242/jcs.00970

[2] Schmitt, B. et al. BMP2 initiates chondrogenic lineage development of adult human mesenchymal stem cells in high-density culture. Differentiation. 71, 567–77 (2003). doi.org/10.1111/j.1432-0436.2003.07109003.x

Additional resources

See BMP-2 bioactivity technote

Publications using recombinant human BMP-2 protein (Qk007)

Sex-Specific Chromatin Remodelling Safeguards Transcription in Germ Cells.
In Nature on 8 December 2021 by Huang, T et al.

View publication

Hydrostatic Pressure Promotes Chondrogenic Differentiation and Microvesicle Release from Human Embryonic and Bone Marrow Stem Cells.
In Biotechnology Journal on 18 December 2021 by Luo, L, et al.

View publication

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

Share your thoughts!

Let us know what you think...

Leave a Review Ask a Question Post a Photo

#reviews .contribution-form-wrapper { display: none; }You must be logged in to join the discussion.

What others are saying

There are no contributions yet.

Amazon gift voucher available for growth factor and cytokine reviews

Cart

Cart

Currency