Recombinant human BMP-2 protein (Qk007)

Product: Qk007 Category:

£205.00 – £2,900.00

Vial image BMP-2

Human recombinant BMP-2 protein (bone morphogenetic protein 2) protein is member of the TGFβ family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.

Qkine recombinant BMP-2 is a 26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of human BMP-2 protein, animal origin-free (AOF) and carrier protein-free.

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£205.00 – £2,900.00

1000µg will be despatched as 2 x 500µg

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Summary:

  • High purity human bone morphogenetic protein 2, BMP-2 (Uniprot: P12643)
    •
  • 26 kDa (dimer)

  • >98%, by SDS-PAGE quantitative densitometry

  • Expressed in E. coli

  • Animal origin-free (AOF) and carrier protein-free

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from acetonitrile, TFA

  • Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Handling and storage FAQ

Featured applications:

  • Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes

Frequently used together
Alternative proteins

BMP-2 activity was determined using the BMP-2 responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 0.3 nM (7.8 ng/mL). Data from Qk007 lot #010.

BMP-2 migrates as a single band at 26 kDa in non-reducing (NR) conditions and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk007 lot #010.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Recovery from stock vial:  >95%

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

MSDS file

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine.  All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Qkine BMP-2 is more biologically active than a comparable alternative supplier protein. Bioactivity was determined using a BMP-2-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of Qkine BMP-2 (Qk007, green) or alternative BMP-2 (supplier B, black). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk007 lot #204510.

Technote | BMP-2 (Qk007) bioactivity

Protein background

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily. It plays numerous roles in the developing embryo, such as embryonic patterning along the dorso-ventral axis, organogenesis, limb bud formation, and bone and cartilage growth. BMP-2 protein is a potent differentiation factor and directs human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes [1]. Recombinant BMP-2 protein induces bone and cartilage formation in vitro and chondrogenesis in human adult mesenchymal stem cells [2].

Human bone morphogenetic protein 2 (BMP-2) is synthesized as a preproprotein consisting of N-terminal signal peptide, 259 amino acid residue pro-domain and 114 residue mature domain. Proteolytic removal of the propeptide enables mature BMP-2 protein to form active disulfide-linked homodimers, and heterodimers with BMP7.

Mature human BMP-2 protein shares 100% amino acid sequence identity with mouse and rat BMP-2. It also shares 85% amino acid sequence identity with the related protein, BMP-4, but less than 51% identity with other BMPs.

Additional resources

Publications using Recombinant human BMP-2 protein (Qk007)

  • CD1530, selective RARγ agonist, facilitates Achilles tendon healing by modulating the healing environment including less chondrification in a mouse model

    Yimiti D, Uchibe K, Toriyama M et al.

    DOI: doi: 10.1002/jor.26006

  • High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humans

    Stucchi S, Sepulveda-Rincon LP, Dion C et al.

    DOI: https://doi.org/10.1101/2025.01.14.632914

  • Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells

    Luo L, Foster NC, Man KL et al.

    DOI: doi: 10.1002/biot.202100401

  • Sex-specific chromatin remodelling safeguards transcription in germ cells

    Huang TC, Wang YF, Vazquez-Ferrer E et al.

    DOI: doi: 10.1038/s41586-021-04208-5

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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Vial image BMP-2Recombinant human BMP-2 protein (Qk007)
£205.00 – £2,900.00View product