Recombinant human BMP-2 protein (Qk007) Price range: £230.00 through £2,900.00View product
Recombinant human BMP-2 protein (Qk007)
Price range: £230.00 through £2,900.00
Human recombinant BMP-2 protein (bone morphogenetic protein 2) protein is member of the TGFβ family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.
Qkine recombinant BMP-2 is a 26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of human BMP-2 protein, animal origin-free (AOF) and carrier protein-free.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £230.00 through £2,900.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human bone morphogenetic protein 2, BMP-2 (Uniprot: P12643)
- 26 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Differentiation of human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal, neural lineages, and chondrocytes
Frequently used together
- Recombinant human FGF-2 (145 aa) protein (Qk025)
- Recombinant human activin A protein (Qk001)
- Recombinant mouse noggin protein (Qk033)
- Recombinant human noggin protein (Qk034)
- Recombinant porcine EGF protein (Qk064)
- Recombinant mouse EGF protein (Qk066)
- Recombinant human FGF-18 protein (Qk069)
- Recombinant human TGF-β2 protein (Qk072)
Alternative proteins
BMP-2 activity was determined using the BMP-2 responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 0.3 nM (7.8 ng/mL). Data from Qk007 lot #010.
BMP-2 migrates as a single band at 26 kDa in non-reducing (NR) conditions and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk007 lot #010.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine BMP-2 is more biologically active than a comparable alternative supplier protein. Bioactivity was determined using a BMP-2-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of Qkine BMP-2 (Qk007, green) or alternative BMP-2 (supplier B, black). Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk007 lot #204510.
Technote | BMP-2 (Qk007) bioactivityProtein background
Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily. It plays numerous roles in the developing embryo, such as embryonic patterning along the dorso-ventral axis, organogenesis, limb bud formation, and bone and cartilage growth. BMP-2 protein is a potent differentiation factor and directs human pluripotent stem cells towards extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes [1]. Recombinant BMP-2 protein induces bone and cartilage formation in vitro and chondrogenesis in human adult mesenchymal stem cells [2].
Human bone morphogenetic protein 2 (BMP-2) is synthesized as a preproprotein consisting of N-terminal signal peptide, 259 amino acid residue pro-domain and 114 residue mature domain. Proteolytic removal of the propeptide enables mature BMP-2 protein to form active disulfide-linked homodimers, and heterodimers with BMP7.
Mature human BMP-2 protein shares 100% amino acid sequence identity with mouse and rat BMP-2. It also shares 85% amino acid sequence identity with the related protein, BMP-4, but less than 51% identity with other BMPs.
Additional resources
Publications using Recombinant human BMP-2 protein (Qk007)
-
CD1530, selective RARγ agonist, facilitates Achilles tendon healing by modulating the healing environment including less chondrification in a mouse model
Yimiti D, Uchibe K, Toriyama M et al.
DOI: doi: 10.1002/jor.26006 -
High resolution multi-scale profiling of embryonic germ cell-like cells derivation reveals pluripotent state transitions in humans
Stucchi S, Sepulveda-Rincon LP, Dion C et al.
DOI: https://doi.org/10.1101/2025.01.14.632914 -
Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells
Luo L, Foster NC, Man KL et al.
DOI: doi: 10.1002/biot.202100401 -
Sex-specific chromatin remodelling safeguards transcription in germ cells
Huang TC, Wang YF, Vazquez-Ferrer E et al.
DOI: doi: 10.1038/s41586-021-04208-5
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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