Recombinant bovine/porcine FGF-2 (154 aa) protein (Qk056)

Name: Recombinant bovine/porcine FGF-2 (154 aa) protein (Qk056)
SKU: Qk056
Availability: InStock

Recombinant bovine/porcine FGF-2 protein 154 aa (bFGF/basic FGF), this is the long form of FGF-2 used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pig) cultivated meat and veterinary research applications. Used in comparative media optimization studies with bovine/porcine FGF-2 145 aa (Qk040).

FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary cell culture.

High purity 16 kDa FGF-2 / bFGF 154 aa protein, animal origin-free (AOF) and carrier-protein free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1000µg will be despatched as 2 x 500µg

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For any questions, please email orders@qkine.com

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Description

Summary

High purity recombinant bovine/porcine FGF-2 protein (154 aa) / bFGF (Uniprot: P03969)
>98%, by SDS-PAGE quantitative densitometry
17.1 kDa
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK facility
Lyophilized from Tris/NaCl/CyS/mannitol
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired, store frozen at -20 ^oC (short-term) or -80 ^oC (long-term)

Handling and storage FAQ

Featured applications

Bovine and porcine stem cell expansion and maintenance; primary cell culture; cellular agriculture process development
Serum-free media development

Alternative protein names

Basic fibroblast growth factor, bFGF, FGFβ, FGF2, Fibroblast growth factor-basic, HBGF2

Species reactivity

bovine, porcine

species similarity:
human – 99%

Alternative proteins

Recombinant porcine/bovine FGF-2 (145 aa) protein (Qk040)
Recombinant human FGF-2 (145 aa) protein (Qk025)
Recombinant human FGF 2 (154 aa) protein (Qk027)
Recombinant zebrafish FGF-2 protein (Qk002)
Recombinant mouse FGF-2 protein (Qk042)

Related discovery kits

FGF-2 species-specific discovery kit (Qk501)
Beefy9 media discovery kit (Qk504)

This protein has recently been reformulated and will now arrive lyophilized. This change has been made to provide enhanced stability to ensure that we offer the best customer experience in terms of shipping and storage and to align with our company sustainability goals. Please visit our reconstitution page or contact customerservice@qkine.com for further information or to request the previous datasheet.

Bioactivity

FGF-2 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 85 pg/ml (5 pM). Cells are treated in triplicate with a serial dilution of FGF-2 for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk056 lot #104386. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

Recombinant bovine/porcine FGF-2 protein (154 aa) migrates as a single major band at 16 kDa in non-reducing (NR) conditions and some dimeric protein migrating at 32 kDa. Upon reduction (R), only the 16 kDa band is visible. No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk056 batch #104386.

Further quality assays

Mass spectrometry: single species with expected mass
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%

Request CoA/animal-free certificate

Download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of porcine (pig) and bovine (cow) embryonic stem cells and proliferation, and differentiation of induced pluripotent and mesenchymal stem cells [1-3]. FGF-2 is an essential growth factor in cultivated meat stem cell, or primary cell proliferation culture media. The two forms of recombinant bovine/porcine FGF2 protein 154 aa (Qk056) and 145 aa (Qk040) available from Qkine facilitate the development of fully optimized serum-free culture media for cellular agriculture applications.

Background references

[1] Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006). doi.org/10.1038/nbt1177

[2] Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006). doi.org/10.1038/nmeth902

[3] Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012). doi.org/10.1002/stem.1021

Additional resources

See bovine/porcine FGF2 technote

Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.

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