Recombinant bovine/porcine FGF-2 (154 aa) protein (Qk056) Price range: £150.00 through £735.00View product
Recombinant bovine/porcine FGF-2 (154 aa) protein (Qk056)
Price range: £150.00 through £735.00
Recombinant bovine/porcine FGF-2 protein 154 aa (bFGF/basic FGF), this is the long form of FGF-2 used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pig) cultivated meat and veterinary research applications. Used in comparative media optimization studies with bovine/porcine FGF-2 145 aa (Qk040).
FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary cell culture.
High purity 16 kDa FGF-2 / bFGF 154 aa protein, animal origin-free (AOF) and carrier-protein free (CF).
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £150.00 through £735.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity recombinant bovine/porcine FGF-2 protein (154 aa) / bFGF (Uniprot: P03969)
- 17.1 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Bovine and porcine primary cell culture
Bovine and porcine stem cell expansion and maintenance
Cellular agriculture process development
Serum-free media development
Related discovery kits
FGF-2 activity was determined using the Promega serum response element luciferase reporter assay in transfected HEK293T cells. EC50 = 85 pg/ml (5 pM). Cells were treated in triplicate with a serial dilution of FGF-2 for 3 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk056 lot #104386.
Recombinant bovine/porcine FGF-2 protein (154 aa) migrates as a single major band at 16 kDa in non-reducing (NR) conditions and some dimeric protein migrating at 32 kDa. Upon reduction (R), only the 16 kDa band is visible. No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk056 batch #104386.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine bovine/porcine FGF-2 was found to be more bioactive than that from an alternative major supplier. Bioactivity was determined using the Promega serum response element luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of Qkine FGF-2 (Qk056, green) or alternative supplier FGF-2 (Supplier B, black) for 3 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. Data from Qk056 lot #104386.
Product background
Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of porcine (pig) and bovine (cow) embryonic stem cells and proliferation, and differentiation of induced pluripotent and mesenchymal stem cells [1-3]. FGF-2 is an essential growth factor in cultivated meat stem cell, or primary cell proliferation culture media. The two forms of recombinant bovine/porcine FGF-2 protein 154 aa (Qk056) and 145 aa (Qk040) available from Qkine facilitate the development of fully optimized serum-free culture media for cellular agriculture applications.
Additional resources
- Technote | bovine/porcine FGF-2 (Qk056) bioactivity vs alternative supplier
- Differentiation of induced pluripotent stem cells (iPSCs) into neuroectoderm (PDF)
- Differentiation of induced pluripotent stem cells (iPSCs) into mesoderm (PDF)
- (PDF)
- Species-specific and optimized growth factors for the production of bovine and porcine cultivated meat and fat (PDF)
- CytoBoost™-enhanced growth factor activity for cell culture and bioprocessing (PDF)
FAQ
What is FGF-2?
Fibroblast growth factor 2 (FGF-2), also known as basic fibroblast growth factor (bFGF) is a growth factor and signaling protein.
Where is FGF-2 found?
FGF-2 is expressed in a developmental and tissue specific manner. It’s expression is tightly controlled in normal tissues and it can be detected in all major tissues.
What does FGF-2 do?
FGF-2 is essential for normal embryonic development. It has roles in cell survival and proliferation, angiogenesis, tumorigenesis, wound healing and tissue repair.
What does FGF-2 bind to?
FGF-2 binds to and signals though all four of the FGF receptors FGFR1-4.
What is the function of the FGF receptor?
FGFRs phosphorylate specific tyrosine residues and activate the RAS-MAPK, PI3K-AKT, PLCγ, and STAT intracellular signaling pathways.
How is FGF-2 used in stem cell culture?
FGF-2 is used to maintain the pluripotency of stem cells in culture.
What is the difference between FGF-2 145 aa and 154 aa?
Fibroblast Growth Factor 2 (FGF-2) exists in two main isoforms, distinguished by length: 145 amino acids (aa) and 154 aa. The 154 aa variant contains an additional nine amino acids at the N-terminus. While this extension is not essential for biological activity, it may influence the protein’s localization within cells.
Key differences between the isoforms:
- FGF-2 (145 aa):
- The most widely used form, supporting proliferation and survival of stem and progenitor cells.
- Commonly preferred for organoid culture due to its robust activity and well-established performance.
- FGF-2 (154 aa):
- Contains an N-terminal extension that may affect intracellular localization or signaling.
- Typically, not required for standard organoid or stem cell maintenance, unless specific signaling or localization effects are being investigated.
What is FGF2-G3 (FGF2-STAB®)?
Recombinant FGF2-G3 (FGF2-STAB®) is a thermostable, engineered form of FGF-2 (bFGF). This modification significantly increases the protein’s functional half-life — from <10 hours for wild-type FGF-2 to >7 days for FGF2-G3 — improving stability and consistency in culture systems.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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