Find the recipe for Beefy-9 here.
The innovation of adding recombinant albumin to B8 media has yielded a groundbreaking advancement in cell culture for the growth of bovine satellite cells, resulting in the development of Beefy-9 media [1-2]. This novel medium is the first of its kind, meeting fundamental criteria to serve as a foundational medium for cultured meat production. One key feature includes the complete absence of animal-derived components, ensuring animal-component-free culture conditions. Even, vitronectin, crucial for cell adhesion to culture flasks, is produced without animal inputs. This departure from traditional serum-containing media, which often rely on animal-derived additives, distinguishes Beefy-9’s commitment to ethical and sustainable practices in cell culture  [3-4].
Beefy-9 incorporates food-safe components, excluding any chemicals or compounds, such as synthetic steroids, that could potentially raise regulatory concerns regarding food safety. This feature enhances confidence in the safety and suitability of cultured meat products developed using Beefy-9 [1,5].
The transparency of Beefy-9’s recipe distinguishes it from previous serum-free media options. Its fully described and defined composition, devoid of proprietary ingredients, facilitates reproducibility and allows for easy modification and optimization. Laboratories interested in advancing cultured meat research can readily adopt and build upon Beefy-9 without facing hurdles related to complex formulations or proprietary restrictions .